Relationship between Helicobacter pylori and gastric carcinoma. -experimental study in H.pylori infected Mongolian gerbil model-

幽门螺杆菌与胃癌的关系。

• 批准号: 08457170
• 负责人: FUJIOKA Toshio
• 金额: $ 2.94万
• 依托单位: Oita Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457170/
• 关键词: animal model; gastric ulcer; atrophic gastritis; intestinal metaplasia; gastric carcinoma; Mongolian gerbil; 胃癌; Helicobacter pylori

Helicobacter pylon is classified by IARC/WHO as a definite human gastric carcinogen, despite "inadequate experimental evidence". To obtain direct evidence concerning this relationship, we investigated the histopathological findings of gastric mucosa using a model of H.pylori infection in Mongolian gerbils. The animals were challenged p. o. with H.pylori ATCC-43504 and sacrificed at 1, 2, 3, 6, 12, and 18 months after inoculation for histological examination. The spiral bacteria were observed in the mucus and gastric pits of all infected animals.A severe infiltration of the lamina propria by polymorphonuclear and mononuclear cells was seen 1 month after H.pylori inoculation. The submucosa was infiltrated by mainly mononullear cells with formation of lymphoid follicles 2 and 3 months after inoculation. Gastric ulcer at the pyloric area in proximity to the fluidus, atrophic gastritis and focal intestinal metaplasia in the lesser curvature of the antrum appeared at 6 months after inoculation.Intestinal metaplasia became severe, with dysplasia, after that. At 18 mnoths after H.pylori inoculation, two of five infected animals showed three well-differentiated gastric adenocarcinomas. The uninfected control animals showed no abnormal findings throughout the entire observation period.This study shows gastric carcinogenesis in a H.pylori-infected Mongolian gerbil model and confirms the IARC/WHO report that H.pylori is a definite human gastric carcinogen.

幽门螺杆菌被国际癌症研究机构/世界卫生组织列为明确的人类胃癌致癌物，尽管“实验证据不足”。为了获得这种关系的直接证据，我们使用蒙古沙鼠幽门螺杆菌感染模型研究了胃粘膜的组织病理学结果。分别于接种后1、2、3、6、12、18个月处死，用ATCC-43504幽门螺杆菌攻毒p.o.，进行组织学检查。所有感染动物的粘液和胃窝中均可见螺旋菌。接种幽门螺杆菌1个月后，可见多形核细胞和单核细胞严重浸润固有层。接种后2、3个月粘膜下层以单核细胞为主浸润，形成淋巴滤泡。在接种后6个月，胃液附近幽门区出现胃溃疡、萎缩性胃炎和胃窦小弯灶性肠化生。此后肠化生严重，发育不良。在接种幽门螺杆菌18个月后，5只感染动物中有2只显示出3种分化良好的胃腺癌。未感染的对照动物在整个观察期间未出现异常。本研究显示幽门螺杆菌感染的蒙古沙鼠模型发生胃癌，证实了IARC/WHO关于幽门螺杆菌是一种明确的人类胃癌致癌物的报告。

项目成果

藤岡利生(分担): "新興再興感染症" 日本医事新報社, 186 (1997)

Toshio Fujioka（撰稿人）：“新出现和重新出现的传染病” Nihon Iji Shinposha，186（1997）

Satoh K, Kimura K, Taniguchi Y, et al: "Biopsy sites suitable for the diagnosis of Helicobacter pylori infection and the assessment of the extent of atrophic gastritis." Am J Gastroenterol. 93. 569-573 (1998)

Satoh K、Kimura K、Taniguchi Y 等人：“适合诊断幽门螺杆菌感染和评估萎缩性胃炎程度的活检部位。”

Kodama M,et al.: "p53 expression in gastric micosa with Helicobacter pylori in fectgon" Journal of Gasfroenterology and Hepatology. 13. 215-219 (1998)

Kodama M 等人：“fectgon 中幽门螺杆菌在胃粘膜中的 p53 表达”《胃肠病学和肝病学杂志》。

Toshio Fujioka et al: "Long-term sequelae of experimental gastritis with Helicobacter pylori:A 5-year follow-up study." J.Clin Gasfroenterol.25(suppl1). 8-12 (1997)

Toshio Fujioka 等人：“幽门螺杆菌实验性胃炎的长期后遗症：一项 5 年随访研究。”

藤岡利生: "注目される感染症-ヘリコバクター・ピロリ-" 臨床科学. 33(9). 1125-1130 (1997)

Toshio Fujioka：“引起关注的传染病 - 幽门螺杆菌”临床科学 33(9) (1997)。