权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Detection of Boma disease virus genome in autopsy brains among people with psychiatric disoders

精神疾病患者尸检大脑中博马病病毒基因组的检测

基本信息

批准号：
08457254
负责人：
IKEDA Kazuhiko
金额：
$ 4.86万
依托单位：
Tokyo Institute of Psychiatry
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457254/
关键词：
Boma disease virus schizophrenia autopsy brain tissue RT-PCR viral infection central nervous system p24

项目摘要

Boma disease virus (BDV), a highly neurotropic virus naturally infects horses and sheep, has been implicated as one of the causative agents for human psyhciatric disorders by the seropeidemiology. To address this possibility, we examined autopsy brain samples from people with psychiatric disorders by means of a highly sensitive nested RT-PCR method for BDV p24, p40 and p10.BDV p24 and p40 PCR products were detected in three cases of 17 brains (frontal and temporal cortices, hippocampus, and olfactory bulb) of people with schizophrenia and one case of 19 control brains. However, BDV p10 PCR produtcs were not found in all the cases. Next, we examined 60 autopsy brains (frontal and temporal cortices) from 45 people with psychiatric disorders and 15 controls, that were provided by the Stanley Foundation Brain Bank Among 120 samples, no PCR products were found for p24 and p40 mRNAs. Given the apparent discordance between our two stuideis, we reexamined brain tissues from 44 people with schizophrenia and 50 controls in a mulitcenter, blinded manner using a collection of total RNA samples prepared in our institute. Three research institutes that participated in the examination gave a similar results with a few positive cases both for p24 and p40. However, p10 PCR products were not obatined in these positive samples, and the sequencing of the PCR products revealed that the products were almost identical with those obtained from the plasmids containing p24 or p40 cDNA that were used in our laboratory. Thus, it is most likely that our initial resutls were pseudopositive, the situation being presumably brought about by the laboratory contamination. Nevertheless, this kind of study should be continued further, with special cautions for contamination.

博马病病毒（BDV）是一种天然感染马和羊的高度嗜神经病毒，已被血清流行病学认为是人类精神疾病的病原体之一。为了解决这一可能性，我们通过一种高度敏感的巢式RT-PCR方法检测了精神疾病患者的尸检脑样本BDV p24， p40和p10。在3例精神分裂症患者的17个大脑（额叶和颞叶皮质、海马和嗅球）和1例对照患者的19个大脑中检测到BDV p24和p40 PCR产物。但并没有在所有病例中发现BDV p10 PCR产物。接下来，我们检查了来自斯坦利基金会脑库提供的45名精神疾病患者和15名对照者的60个尸检大脑（额叶和颞叶皮质）。在120个样本中，没有发现p24和p40 mrna的PCR产物。鉴于我们两个研究之间的明显不一致，我们采用多中心盲法重新检查了44名精神分裂症患者和50名对照组的脑组织，使用了我们研究所准备的总RNA样本。参与检查的3个研究所也得出了类似的结果，p24和p40都出现了少数阳性病例。然而，在这些阳性样本中没有获得p10 PCR产物，并且PCR产物的测序结果显示，PCR产物与我们实验室使用的含有p24或p40 cDNA的质粒获得的产物几乎相同。因此，很可能我们最初的结果是假阳性的，这种情况可能是由实验室污染造成的。然而，这种研究应该继续下去，并特别注意污染。