Resesrch of prognostic factor on biopsy specimen for indication of partial nephrectomy

本研究的目的是评价选择性肾肿瘤活检在肾癌患者中的疗效。本文对1985 ~ 1994年41例肾细胞癌患者在肾部分或根治性切除术前进行了选择性肾肿瘤活检，并对活检组织DNA含量与手术标本DNA含量的关系进行了分析。应用静态细胞荧光法（SCM）和流式细胞术（FCM）对41例肾癌活检标本和手术标本进行石蜡块DNA含量检测，结果表明：SCM和FCM分别有39例和37例能分析活检标本的DNA含量。肾细胞癌选择性肾肿瘤活检结果与手术病理结果一致者27/39（准确度69%，kappa 0.398），29/39结构类型26/39例，准确率74%，Kappa值0.432 DNA直方图在分级中的准确率为67%（kappa 0.341），在倍体类型中的准确率为69%（kappa 0.532）。

The object of this study is to evaluate the efficacy of selectiverenal tumor biopsy in patients with renal cell carcinoma. The accuacy of histological diagnosis and DNA content of biopsies in predicting those of surgical specimens was assessed.From 1985 to 1994,41 patients with renal cell carcinoma under went selectiverenal tumor biopsy before partial or radical nephrectomy. DNA content of renal cell carcinoma in 41 patients in biopsy specimens and surgical specimens were examined in paraffin blocks with static cytofluorometry (SCM) and Flow cytometry (FCM).In 39 out of 41 cases, by SCM,as well as in 37 out of 41 cases by FCM,DNA content in biopsy could be analyzed. Findings of selective renal tumor biopsy for RCC coincided with those of surgical specimen in 27/39 (accuracy 69%, kappa 0.398) in cell types, 29/39 (accuracy 74%, kappa 0.432) in structural type, 26/39 (accuracy 67%, kappa 0.341) in gradings and 27/39 (accuracy 69%, kappa 0.532) in ploidy patterns.It was assumed that DNA histograms might help the histological diagnosis by biopsy.