Resesrch of prognostic factor on biopsy specimen for indication of partial nephrectomy

本研究的目的是评价选择性肾肿瘤活检术对肾癌患者的疗效。对1985~1994年间41例肾细胞癌患者行肾部分或根治性肾切除术前行选择性肾肿瘤活检术，评价组织学诊断和活检DNA含量预测手术标本的准确性。应用静态细胞荧光法(SCM)和流式细胞术(FCM)对41例肾细胞癌活检标本和手术标本的DNA含量进行了检测，其中SCM法检测39例，FCM法检测37例。肾细胞癌选择性肾肿瘤活检与手术标本符合27/39(准确率69%，kappa 0.398)，29/39(准确率74%，kappa 0.432)，26/39(准确率67%，kappa 0.341)，27/39(准确率69%，kappa 0.532)。

The object of this study is to evaluate the efficacy of selectiverenal tumor biopsy in patients with renal cell carcinoma. The accuacy of histological diagnosis and DNA content of biopsies in predicting those of surgical specimens was assessed.From 1985 to 1994,41 patients with renal cell carcinoma under went selectiverenal tumor biopsy before partial or radical nephrectomy. DNA content of renal cell carcinoma in 41 patients in biopsy specimens and surgical specimens were examined in paraffin blocks with static cytofluorometry (SCM) and Flow cytometry (FCM).In 39 out of 41 cases, by SCM,as well as in 37 out of 41 cases by FCM,DNA content in biopsy could be analyzed. Findings of selective renal tumor biopsy for RCC coincided with those of surgical specimen in 27/39 (accuracy 69%, kappa 0.398) in cell types, 29/39 (accuracy 74%, kappa 0.432) in structural type, 26/39 (accuracy 67%, kappa 0.341) in gradings and 27/39 (accuracy 69%, kappa 0.532) in ploidy patterns.It was assumed that DNA histograms might help the histological diagnosis by biopsy.