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Quantitative analysis of Epstein-Barr virus immediate-early proteins and evaluation of its clinical role especially in nasopharyngeal

EB病毒立即早期蛋白的定量分析及其临床作用（尤其是在鼻咽部）的评估

基本信息

批准号：
08457450
负责人：
FURUKAWA Mitsuru
金额：
$ 4.93万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457450/
关键词：
NPC EBV BZLF1 BRFL1 Western blotting ELISA

项目摘要

Nasopharyngeal carcinoma (NPC), an epithelial tumor which is characterized by marked geographic and population differences in incidence, is found to be associated with Epstein-Barr virus (EBV) by serologic evidence, and the relationship was confirmed by the detection of EBVDNA and EB-encoded RNAs in NPC cells. Elevation of lgG and lgA antibodies against EBV viral capsid antigen and early antigen are often found in NPC patients. Serologic tests of these antibodies have been recognized astumor markers for NPC.EBV persists in a latent form in both epithelial cells and B lymphocytes. A variety of stimulation may activate a virus. The Z protein and the R protein, which are immediate early proteins of the EBV lytic cycle, control the switch of the virus from a latent to a lytic cycle by transactivating several early promoters. But a lytic cycle is sometimes incomplete in tumor. Z protein and R protein are expressed earlier than EA or VCA,so anti-Z,-R antibody titers will be more sensitive markers for NPC.Plasmid presenting BZLF1 or BRLF1 gene were produced, and Z protein and R protein were harvested. Sera from patients with NPC,infectious monomucleosis (IM), non-Hodgkin's lyumphoma (NHL), head and neck carcinoma and healthy controls are examined for its reactivity against Z and R protein by Western blotting and ELISA.NPC patients before treatment all indicated high anti-Z,-R antibody titers, whereas in three NPC patients without recurrence, only one (33%) was positive. IM patients all indicated no anti-Z,-R antibodies. Several patients of other diseases have anti-Z,-R antibodies, but their titers were low. In comparison with anti-VCA antibodies, ant-Z,-R antibodies were specific to NPC.A NPC patients whose anti-VCA antibodies were negative indicated high anti -Z,-R antibody titers, and a NHL patient who shows elevation of anti-VCA antibody titer was negative in anti-Z,-R antibodies. These findings suggest that the anti-Z,-R antibody titers are useful markers for NPC.

鼻咽癌（NPC）是一种发病率具有明显地域和人群差异的上皮性肿瘤，血清学证据表明鼻咽癌与eb病毒（EBV）有关，并通过鼻咽癌细胞中EBVDNA和eb编码rna的检测证实了这种关系。针对EBV病毒衣壳抗原和早期抗原的lgG和lgA抗体升高常见于鼻咽癌患者。这些抗体的血清学试验已被认为是鼻咽癌的肿瘤标志物。eb病毒在上皮细胞和B淋巴细胞中以潜伏形式存在。各种刺激都可能激活病毒。Z蛋白和R蛋白是eb病毒裂解周期的直接早期蛋白，它们通过反激活几个早期启动子来控制病毒从潜伏到裂解周期的转换。但肿瘤的溶解周期有时是不完整的。Z蛋白和R蛋白的表达早于EA或VCA，因此抗Z、-R抗体滴度将是鼻咽癌更敏感的标志物。制备含BZLF1或BRLF1基因的质粒，收获Z蛋白和R蛋白。采用免疫印迹法（Western blotting）和ELISA检测鼻咽癌、传染性单核细胞增多症（IM）、非霍奇金淋巴瘤（NHL）、头颈部癌和健康对照患者血清中Z和R蛋白的反应性。治疗前鼻咽癌患者抗z、-R抗体滴度均较高，而3例未复发的鼻咽癌患者中仅有1例（33%）呈阳性。IM患者均无抗z、-R抗体。少数其他疾病患者有抗z、-R抗体，但滴度较低。与抗vca抗体相比，抗z、-R抗体对鼻咽癌具有特异性。抗vca抗体阴性的NPC患者抗z、-R抗体滴度高，抗vca抗体滴度升高的NHL患者抗z、-R抗体滴度阴性。这些结果提示抗z，-R抗体滴度是鼻咽癌的有用标记。