Molecular biological analysis of Siah1 in EBV related tumor including nasopharyngeal cancer

Siah1在鼻咽癌等EBV相关肿瘤中的分子生物学分析

• 批准号: 19390433
• 负责人: FURUKAWA Mitsuru
• 金额: $ 11.56万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390433/
• 关键词: 上咽頭癌; バーキットリンパ腫; 浸潤; 転移; Siah1

We have shown that EBV-LMP1 that induced nasopharyngeal cancer (NPC), stabilizes HTF1α via Siah1in nasopharyngeal epithelial cells. However LMP1 downregulates Siah1 in Burkitt lymphoma cell lines. In this study, we examined Siah1 function in various EBV-related tumors. We investigated expression of LMP1, HIF1α, and Siah1 by immunohistochemistry. Finally we found that the expression of Siah1 was correlated with LMP1 strongly in NPC. However there was no correlation in other EBV-related tumors. Since we hypothesize not only LMP1 inducible stimulation but hypoxia condition itself also affects HIF1-α expressions in NPC tissues, we investigated with LMP1 expressed nasopharyngeal cell lines with or without hypoxia stimulation with hypoxia chamber. Synergistic effect of hypoxic conditions and LMP1 expression increased their HIF1α expression in vitro and in vivo using immunodeficinet mice. The crosstalk of hypoxia and LMP1 expression may be a key of understanding EBV-related tumors.

我们已经证明，诱导鼻咽癌（NPC）的EBV-LMP 1通过Siah 1稳定鼻咽上皮细胞中的HTF 1 α。然而，LMP 1下调伯基特淋巴瘤细胞系中的Siah 1。在这项研究中，我们研究了Siah 1在各种EBV相关肿瘤中的功能。我们通过免疫组织化学研究了LMP 1、HIF 1 α和Siah 1的表达。结果发现Siah 1与LMP 1在鼻咽癌中的表达呈强相关。然而，在其他EBV相关肿瘤中没有相关性。由于我们假设HIF 1-α的表达不仅受LMP 1诱导的刺激影响，而且缺氧条件本身也会影响HIF 1-α的表达，因此我们用低氧箱研究了LMP 1表达的鼻咽癌细胞系在缺氧刺激和无缺氧刺激下的表达。缺氧条件和LMP 1表达的协同作用增加了它们在体外和体内使用免疫缺陷小鼠的HIF 1 α表达。缺氧与LMP 1表达的相互影响可能是理解EBV相关肿瘤的关键。

项目成果

T3喉頭癌に対する超選択的動注化学療法後の声帯可動性

T3 喉癌超选择性动脉内化疗后声带活动度

• 发表时间: 2008
• 作者: 室野重之;近藤悟;脇坂尚宏;吉崎智一;古川仭
• 通讯作者: 古川仭

A solitary fibrous tumor arising in the parapharyngeal space, with MRI and FDG-PET finding.

MRI 和 FDG-PET 发现咽旁间隙出现孤立性纤维瘤。

• 发表时间: 2008
• 期刊: Auris Nasus Larynx (In Press)
• 作者: Wakisaka N;Kondo S;他3名
• 通讯作者: 他3名

Impact of MDM2 single nucleotide polymorphism on tumor onset in head and necsquamous cell carcinoma.

MDM2 单核苷酸多态性对头部和鳞状细胞癌肿瘤发生的影响。

• 发表时间: 2008
• 期刊: Acta Otolaryngologica 128
• 作者: Nakashima M;Kondo S.Furukawa M.
• 通讯作者: Kondo S.Furukawa M.

Epstein-Barr Virus latent membrane protein 1 induces Snail and epithelial-mesenchymal transition in metastatic nasopharyngeal carcinoma.

• DOI: 10.1038/bjc.2011.38
• 发表时间: 2011-03-29
• 期刊: BRITISH JOURNAL OF CANCER
• 影响因子: 8.8
• 作者: Horikawa, T.;Yoshizaki, T.;Kondo, S.;Furukawa, M.;Kaizaki, Y.;Pagano, J. S.
• 通讯作者: Pagano, J. S.

A single nucleotide polymorphism in the matrix metalloproteinase-1 and interleukin-8 gene promoter predicts poor prognosis in tongue cancer.

• DOI: 10.1016/j.anl.2007.12.002
• 发表时间: 2008-09
• 期刊: Auris, nasus, larynx
• 作者: Y. Shimizu;S. Kondo;Akiko Shirai;M. Furukawa;T. Yoshizaki