Molecular mechanisms of ion channels and second messengers in gustatory transduction of taste cells.
味觉细胞味觉转导中离子通道和第二信使的分子机制。
基本信息
- 批准号:08457491
- 负责人:
- 金额:$ 4.48万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. When inward currents in the fungiform papilla taste cells induced by NaCi stimuli were analysed, it was found that the taste cells in the mouse were divided into two types according to the presence and absence or almost absence of amiloride-blockable epithelial Na^+ channels (ENaC) in the apical receptive membrane. When large inward currents elicited by NaCi stimuli were blocked by amiloride, the subunits of ENaC may be composed of alpha, beta and gamma. However,when the inward currents were not blocked completely or almost completely by amiloride, it is likely that the apical membrane of a taste cell lacks the whole subunits or beta and gamma of ENaC.2. The inward current in the mouse induced by an acid stimulus was not blocked at all by amiloride. Therefore, the acid response of mouse taste cells is not evoked via the amiloride-blockable ENaC.Our recent finding suggests that there are two pathways for generation of acid responses. One is a cascade of binding of proton to ionotoropic receptor in the apical membrane and inflow of cations through the cation channels. The other pathway is a cascade of activation of G-protein and effector molecule, production of a second messenger and closure of C1^- channels in the basolateral membrane via a protein kinase.3. Two pathways are concerned with generation of sweet responses in gerbil taste cells for two types of sweeteners. One pathway for sugar stimuli is a cascade of activation of G-protein and adenylate cyclase, production of cAMP and closure of K^+ channels via a protein kinase. The other pathway for non-sugar sweeteners is a cascade of activation of G-protein and PLC, production of 1P3 and release of Ca^2^+ from the intracellular Ca^2^+ store.
1.当分析NaCl刺激引起的菌状乳头味细胞的内向电流时,发现小鼠的味细胞根据顶端感受膜中存在和不存在或几乎不存在阿米洛利可阻断的上皮Na ^+通道(ENaC)而分为两种类型。当阿米洛利阻断NaCl刺激引起的大内向电流时,ENaC的亚基可能由α、β和γ组成。然而,当内向电流没有完全或几乎完全被阿米洛利阻断时,很可能味觉细胞的顶端膜缺乏ENaC的整个亚基或β和γ。阿米洛利完全不能阻断小鼠由酸刺激诱导的内向电流。因此,小鼠味觉细胞的酸反应不是通过阿米洛利可阻断的ENaC诱发的。我们最近的发现表明,有两种途径产生酸反应。一个是质子与顶膜中的离子受体结合和阳离子通过阳离子通道流入的级联反应。另一个途径是通过蛋白激酶激活G蛋白和效应分子、产生第二信使和关闭基底外侧膜中的C1 ^-通道的级联反应。沙鼠味觉细胞对两种甜味剂产生甜味反应的途径有两种。糖刺激的一个途径是通过蛋白激酶激活G蛋白和腺苷酸环化酶、产生cAMP和关闭K ^+通道的级联反应。非糖甜味剂的另一个途径是G蛋白和PLC的激活、1P3的产生和Ca^2 ^+从细胞内Ca^2 ^+库中的释放的级联反应。
项目成果
期刊论文数量(44)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Miyamoto,T.,et al.: "Sour transduction involves activation of NPPB-sensitive conductance in mouse taste cells." J.Neurophysiol.80. 1852-1859 (1998)
Miyamoto,T.,et al.:“酸转导涉及小鼠味觉细胞中 NPPB 敏感电导的激活。”
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- 发表时间:
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- 影响因子:0
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- 通讯作者:
Okada, Y, , et al.: "Vasopressin modulates membrane prooerties of taste cells isolated from bullfrogs." Chem.Senses. 21. 739-745 (1996)
Okada, Y, 等人:“加压素调节从牛蛙中分离出的味觉细胞的膜特性。”
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- 影响因子:0
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Hoshi, T., et al.: Review of Medical Physiology (18 ed.). Maruzen, 851 (1998)
Hoshi, T. 等人:医学生理学评论(第 18 版)。
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- 影响因子:0
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Miyamoto,T.,et al.: "Whole-cell recording from non-dissociated taste cells in mouse taste bud." J.Neurosci.Methods. 64・2. 79-85 (1996)
Miyamoto, T., et al.:“小鼠味蕾中非分离味觉细胞的全细胞记录。”J.Neurosci.Methods 64・2(1996)。
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- 期刊:
- 影响因子:0
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- 通讯作者:
Miyamoto,T.,et al.: "Properties of Na^+-dependent K^+ conductance in the apical membrane of frog taste cells." Brain Res.715. 79-85 (1996)
Miyamoto,T.,et al.:“青蛙味觉细胞顶膜中 Na^ 依赖性 K^ 电导的特性。”
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- 影响因子:0
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{{ truncateString('SATO Toshihide', 18)}}的其他基金
Development of system for analyzing membrane proteins of taste cells ender physiological condition.
开发用于分析味觉细胞膜蛋白的系统以实现生理条件。
- 批准号:
07557117 - 财政年份:1995
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular mechanisms of transduction of gustatory stimuli in human and mammalian tatse cells.
人类和哺乳动物 tatse 细胞味觉刺激转导的分子机制。
- 批准号:
05404063 - 财政年份:1993
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
Distribution, isolation and reconstitution of functional molecules related to the taste-signal transduction.
与味觉信号转导相关的功能分子的分布、分离和重建。
- 批准号:
04557080 - 财政年份:1992
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Comprehensive study on food intake
食物摄入量综合研究
- 批准号:
03304042 - 财政年份:1991
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Co-operative Research (A)
Molecular mechanism of gustatory transduction in taste cells
味觉细胞味觉转导的分子机制
- 批准号:
01480433 - 财政年份:1989
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Transduction mechanism of four basic taste stimuli into electric signals of taste cell
四种基本味觉刺激转变成味觉细胞电信号的转导机制
- 批准号:
60480402 - 财政年份:1985
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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