IDBR: Nanoengineered Enhancements to the Patch-Clamp Technique

IDBR：膜片钳技术的纳米工程增强

• 批准号: 0551852
• 负责人: John Troy
• 金额: --
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0551852&HistoricalAwards=false
• 关键词: IDBR; Nanoengineered; Enhancements; Patch; Clamp

This award supports a project that will improve instrumentation used for patch-clamp measurements for recording the electrical potential of individual cells. The patch-clamp method is the most widely used method for this purpose; 26,000 research publications have cited the method since 1975, half of these appearing in the last five years. Despite this widespread use, the method has a number of well known limitations that arise from the equipment used. In particular, it is difficult to obtain and to sustain more than one recording at a time, making studies of the electrical activity of individual members of cellular networks difficult. Moreover, the quality of recordings deteriorates with time, and the recording bandwidth is limited. Through the development of new instrumentation undertaken with the support of this award, long-duration, multiple-site recordings will be easier to obtain, and high bandwidth signals will be more accessible. Traditional patch clamp systems employ a glass pipette to contact the cell with an Ag/AgCl electrode located at a fixed position far from the pipette tip. Introduction of any cellular or other debris near the tip interferes with reliable measurement. In the device to be developed, a movable nanoelectrode can be advanced forward and through the tip to clear such debris. This modification alone is expected to alleviate most of the current patch clamp limitations. While the proposed device will be more complex than a standard patch clamp electrode, the project's goal is development of a device that will integrate easily into existing patch clamp systems. This approach of adapting the design to systems currently in use should encourage rapid acceptance of the new tool among electrophysiologists, significantly increasing the likely impact it will have on the progress of biological research over the next decade. The PI has been active in development of new curricula and other activities that serve both neuroscience and bioengineering. Because of the extensive use of the patch-clamp in a variety of areas of neuroscience and cell biology, successful development of the proposed device can be expected to have a broad impact on biological research.

该奖项支持一个项目，该项目将改进用于记录单个细胞电位的膜片钳测量仪器。膜片钳方法是最广泛使用的方法;自1975年以来，有26，000份研究出版物引用了该方法，其中一半出现在过去五年中。 尽管这种广泛的使用，该方法有许多众所周知的限制，所使用的设备。 特别是，难以一次获得和维持多于一个的记录，使得难以研究蜂窝网络的个体成员的电活动。此外，记录的质量随时间劣化，并且记录带宽有限。通过在该奖项的支持下开发新的仪器，将更容易获得长时间、多地点的记录，更容易获得高带宽信号。 传统的膜片钳系统采用玻璃移液管将细胞与位于远离移液管尖端的固定位置处的Ag/AgCl电极接触。 在尖端附近引入任何细胞或其他碎片会干扰可靠的测量。 在待开发的装置中，可移动纳米电极可以向前推进并穿过尖端以清除此类碎片。这种修改预计将减轻大多数目前膜片钳的限制。虽然拟议的设备将比标准膜片钳电极更复杂，但该项目的目标是开发一种易于集成到现有膜片钳系统中的设备。这种使设计适应当前使用的系统的方法应该鼓励电生理学家迅速接受新工具，显着增加它对未来十年生物学研究进展的可能影响。 PI一直积极开发新的课程和其他活动，为神经科学和生物工程服务。 由于膜片钳在神经科学和细胞生物学的各种领域中的广泛应用，所提出的设备的成功开发可以预期对生物学研究产生广泛的影响。