Study on the Regulation and Function of Phosphoinositide 3-Kinases
磷酸肌醇3-激酶的调控及功能研究
基本信息
- 批准号:08457605
- 负责人:
- 金额:$ 4.74万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Two types of phosphoinositide 3-kinase (PI 3-kinase), in terms of the mechanism of activation, had been recognized. One is stimulated by a tyrosine-phosphorylated peptide (PY). The other is activated by the betagamma subunits of GTP-binding proteins (GBgamma). In the present study, we found the activity which is stimulated by both PY and GBgamma in a synergistic manner. The activity was identified to be the beta-subtype of PI 3-kinase. In rat adipocyted, cell production of phsphatidylinositol-3,4,5-trisphosphate, a product of PI 3-kinase, increased synergistically by insulin (tyrosine kinase-activating) and adenosine (G-protein-activating). Similar results were observed in the CHO cells artificially transfected with the insulin and fMLP receptors. Insulin actions on these cells (glucose uptake, memrane ruffling) were effectively potentiated by simultaneous stimulation of the GTP-binding proteins. This effect of GTP-binding proteins was accompanied by the potentiation of insulin-induced activation of protein kinase B,a putative downstream target of PI 3-kinases. The results indicated a novel role of GTP-binding proteins, a permissive regulation of the insulin receptor-derived signal.
磷脂酰肌醇3-激酶(PI 3-kinase)的激活机制有两种。一种是由酪氨酸磷酸化肽(PY)刺激。另一种是由GTP结合蛋白(GB γ)的β γ亚基激活。在本研究中,我们发现了PY和GB γ以协同方式刺激的活性。该活性被鉴定为PI 3-激酶的β亚型。在大鼠脂肪细胞,磷脂酰肌醇-3,4,5-三磷酸,PI 3-激酶的产物,协同增加胰岛素(酪氨酸激酶激活)和腺苷(G蛋白激活)的细胞生产。在用胰岛素和fMLP受体人工转染的CHO细胞中观察到类似的结果。胰岛素对这些细胞的作用(葡萄糖摄取,memrane ruffling)有效地增强了GTP结合蛋白的同时刺激。GTP结合蛋白的这种作用伴随着胰岛素诱导的蛋白激酶B(PI 3-激酶的推定下游靶点)活化的增强。结果表明,一种新的作用的GTP结合蛋白,胰岛素受体衍生的信号的一种允许的调节。
项目成果
期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hazeki, O., et al.: "Inhibitory effect of wortmannin on phosphatidylinositol 3-kinase-mediated cellular events. J." Lipid Mediators Cell signalling. 14. 259-261 (1996)
Hazeki, O. 等人:“渥曼青霉素对磷脂酰肌醇 3-激酶介导的细胞事件的抑制作用。J.”
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
Matsuo, T., et al.: "Association of Phosphatidylinositol 3-kinase with the protooncogene product Cbl upon CD38 ligation by a specific monoclonal antibody in THP-1 cells." FEBS Lett.397. 113-116 (1996)
Matsuo, T. 等人:“在 THP-1 细胞中通过特定单克隆抗体连接 CD38 后,磷脂酰肌醇 3-激酶与原癌基因产物 Cbl 的关联”。
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- 发表时间:
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- 影响因子:0
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- 通讯作者:
N. Tsujimoto, et al.: "Potentiation of chemotactic peptide-induced superoxide generation by CD38 ligation in human myeloid cell lines." J. Biochem.121. 949-956 (1997)
N. Tsujimoto 等人:“在人骨髓细胞系中通过 CD38 连接增强趋化肽诱导的超氧化物生成。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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Matsuo, T., et al.: "Activation of phosphatidylinositol 3-kinase by concanavalin A through dual signaling pathways, G protein-coupled and phosphotyrosine-related, and an essential role of the G protein-coupled signals for the lectin-induced respiratory bu
Matsuo, T. 等人:“伴刀豆球蛋白 A 通过 G 蛋白偶联和磷酸酪氨酸相关的双重信号通路激活磷脂酰肌醇 3-激酶,以及 G 蛋白偶联信号对于凝集素诱导的呼吸系统的重要作用
- DOI:
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- 期刊:
- 影响因子:0
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- 通讯作者:
Hazeki, O., et al.: "Activation of PI 3-kinase by G protein betagamma subunits." Life.Sci.62. 1555-1559 (1998)
Hazeki, O., et al.:“G 蛋白 betaamma 亚基激活 PI 3-激酶。”
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- 影响因子:0
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HAZEKI Osamu其他文献
HAZEKI Osamu的其他文献
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{{ truncateString('HAZEKI Osamu', 18)}}的其他基金
Vesicle trafficking in phosphoinositide phosphatase-knockdown cells
磷酸肌醇磷酸酶敲低细胞中的囊泡运输
- 批准号:
23590078 - 财政年份:2011
- 资助金额:
$ 4.74万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Construction of a Bank of the Cells Lacking Phosphoinositide-Metabolizing Enzymes
缺乏磷酸肌醇代谢酶的细胞库的构建
- 批准号:
20590060 - 财政年份:2008
- 资助金额:
$ 4.74万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
SYNERGISTIC REGULATION OF PHOSPHOINOSITIDE 3-KINASE
磷酸肌醇3-激酶的协同调节
- 批准号:
12470502 - 财政年份:2000
- 资助金额:
$ 4.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A Study on an Intracellular Signaling Molecule, Phosphinositide 3-Kinase
细胞内信号分子磷酸肌醇 3-激酶的研究
- 批准号:
06672169 - 财政年份:1994
- 资助金额:
$ 4.74万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
A search with Pertussis Toxin for Cellular Factors Affecting the Properties of GTP-Binding Protein
用百日咳毒素寻找影响 GTP 结合蛋白特性的细胞因素
- 批准号:
04680184 - 财政年份:1992
- 资助金额:
$ 4.74万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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