Role of the Novel Gene, HIC-5, that encodes a Zn-finger Protein in Cellular Differentiation.

编码锌指蛋白的新基因 HIC-5 在细胞分化中的作用。

• 批准号: 08457616
• 负责人: SHIBANUMA Motoko
• 金额: $ 4.54万
• 依托单位: SHOWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457616/
• 关键词: differentiation; Zn finger protein; HIC-5; expression vectors; focal adhesion; Alu配列

Roles of the hic-5 gene that encodes a novel Zn-finger protein in cellular differetiation was examined using sense-and antisense-expression vectors for hic-5. Forced expression of sense-strand of hic-5 did not affect the differentiation processes of myogenic C2C12 or osteoblastic RCT-1 cells, but introduction of antisence expression vectors inhibited osteogenic differentiation as revealed by decrease in expression of extracellular matric proteins and alkaline phosphatase. These sffects seemed to be a result of AP-1 activation through constitutive expression of c-fos gene that was caused by the HIC-5.Immunocytochemical studies using specific antiboby against HIC-5 showed that the protein localize in focal adhesion plaques in normal fibroblasts, and the HIC-5 associates with vinculin, talin and FAK.It was speculated that the HIC-5 protein is a gate keeper for signal transduction pathways from cellular surface to nuclei.

使用hic-5的正义和反义表达载体检测了编码新型锌指蛋白的hic-5基因在细胞凋亡中的作用。hic-5有义链的强制表达不影响肌源性C2 C12或成骨细胞RCT-1细胞的分化过程，但反义表达载体的引入抑制成骨分化，表现为细胞外基质蛋白和碱性磷酸酶的表达减少。这些作用可能是由HIC-5引起的c-fos基因的组成性表达激活AP-1的结果。免疫细胞化学研究表明，HIC-5蛋白定位于正常成纤维细胞的粘着斑，并且与黏着斑蛋白结合，推测HIC-5蛋白是细胞表面至细胞核的信号转导通路的守门人。

项目成果

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M.Shibanuma et al.: "Induction of Senescance-like phenotypes in immortahzed human fibroblasts" Mol.Cell.Biol.印刷中 (1997)

M. Shibanuma 等人：“永生化人类成纤维细胞中衰老样表型的诱导”Mol.Cell.Biol 出版（1997 年）

Arata,S.et al.: "Inhibition of colony formation of NIH3T3 cells by HSP27" J.Cell.Physiol.170. 19-26 (1997)

Arata,S.et al.：“HSP27 对 NIH3T3 细胞集落形成的抑制”J.Cell.Physiol.170。

Arata, S., et al: "Inhibition of colony formation of N11+3T3 cells by HSP27" J. Cell. Physiol.170. 19-26 (1997)

Arata, S. 等人：“HSP27 对 N11 3T3 细胞集落形成的抑制”J. Cell。