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Hormonal Control of Chloride Cell Differentiation in Fish Gills

鱼鳃氯细胞分化的激素控制

基本信息

批准号：
08459008
负责人：
HIRANO Tetsuya
金额：
$ 5.31万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08459008/
关键词：
Osmoregulation Gills Chloride Cells Ion Transport Migratory Fishes Prolactin Cortisol Thyroid Hormones イオン透過性酸性環境

项目摘要

The aim of this study is to clarify endocrine control of differentiation of chloride cells in fish gills in response to the changes in environmental salinity. The results obtained during the past two years are as follows.1. A cDNA of 453 bp was cloned from liver mRNA of tilapia with primers designed for the hormone-binding domain of cortisol receptor of the rainbow trout. The receptor message by Northern blot analysis was stronger in fish in fresh water than in those in seawater. The receptor mRNA was already present in the yolk just after fertilization, indicating its importance in cell and tissue differentiation during the early development.2. Two types of chloride cells were identified in the gill filament of chum salmon. Lamellar chloride cells were mainly present in the fish in fresh water and disappeared during seawater adaptation, suggesting their role as a site for ion uptake in fresh water. On the other hand, filament chloride cells were developed during seawater adaptation, i … More ndicating their role as a site of salt excretion in seawater.3. Cortisol receptor gene expression, examined by in situ hybridization, were found in the two types of chloride cells in the chum salmon fry, indicating that cortisol is one of the important factors regulating chloride cell functions.Cortisol receptors were also present in undifferentiated cells, also suggesting its involvement in the functional differentiation of chloride cells.4. Prolactin receptor gene was cloned from the liver mRNA of tilapia with primers for the extracellular domain of the receptors of mammalian and stenohaline tilapia. Competitive PCR revealed the presence of the receptor in the embryos 2 days after fertilization, and the gene expression was stronger in the embryos developing in fresh water than those in seawater.5. It has becaome increasingly, clear that various hormones such as cortisol and thyroid hormones are contained in the yolk of various fish species, possibly affecting the cell and tissue differentiation. Further studies are called for to examine whether prolactin and thyroid hormones are involved specifically in the differentiation of the gill chloride cells. Less

本研究的目的是阐明内分泌控制的分化氯细胞在鱼的鳃响应环境盐度的变化。在过去两年中取得的成果如下。根据虹鳟鱼皮质醇受体结合区的序列设计引物，从罗非鱼肝脏mRNA中克隆了一个453 bp的cDNA。北方印迹分析表明，淡水鱼的受体信息比海水鱼的强。受体mRNA在受精后即已存在于卵黄中，表明其在早期发育过程中细胞和组织分化中的重要性.两种类型的氯细胞被确定在鳃丝的鲑鱼。板层氯细胞主要存在于淡水中的鱼和海水适应过程中消失，这表明它们的作用作为一个网站的离子吸收在淡水中。另一方面，丝状氯细胞在海水适应过程中发育， ...更多信息表明它们是海水中盐排泄的场所。原位杂交检测到两种氯细胞中都有皮质醇受体基因的表达，表明皮质醇是氯细胞功能的重要调节因子之一;未分化细胞中也有皮质醇受体的表达，提示皮质醇受体参与氯细胞的功能分化.根据哺乳动物和狭盐罗非鱼催乳素受体胞外区的特异性引物，从罗非鱼肝脏mRNA中克隆了催乳素受体基因。竞争性PCR检测表明，受精后2d的胚胎中存在该受体，且在淡水中发育的胚胎中该基因的表达强于海水中发育的胚胎.越来越多的研究表明，各种鱼类的卵黄中含有皮质醇、甲状腺激素等多种激素，可能影响细胞和组织的分化。催乳素和甲状腺激素是否与鳃氯细胞的分化有关还有待于进一步的研究。少