Purification of a novel migration factor for vascular smooth muscle cells, derived from omentum and its application

越来越多的证据表明，脂肪组织具有强大的血管生成活性。在本研究中，我们试图从监测血管平滑肌细胞迁移活性的大网膜中提纯新的血管生成因子。在15小杯甲醇中匀浆3公斤的大网膜。经过液-液分配层析、制备薄层层析和两种高效液相色谱四步纯化，得到了0.3 mg的纯化产物。经快原子轰击质谱仪和核磁共振波谱分析，其相对分子质量为747.9，为1-棕榈酰基-2-油酰磷脂酰甘油。测定了合成的1-棕榈酰基-2-油酰基磷脂酰甘油的迁移活性。我们还考察了其他磷脂的迁移活性。磷脂酰乙醇胺和磷脂酰丝氨酸具有迁移活性，而磷脂酰丝氨酸则不具有迁移活性。在本研究中，1-棕榈酰基-2-从大网膜中分离纯化得到一个迁移因子。

Growing evidence indicates that adipose tissues have potent angiogenic activity. In the present study, we tried to purify the new angionenic factor from omentum monitoring the migration activity of vascular smooth muscle cells. Three kg of omentum was homogenized in the 15 little of methanol. After four steps of purification procedure including liquid- ; liquid partition chromatography, preparative thin-layr chromatography and two kinds of high-performance liquid chromatography, 0.3mg of purified product was obtained. Spectral analysis using fast atom bombardment mass spectrometry (FAB-MAS) ans nuclear magnetic resonance spectrometry revealed its molecular weight is 747.9 and it is 1-Palmitoyl-2-oleoyl-phosphatidylglycerol. Migration activity of synthesized 1-Palmitoyl-2-oleoyl-phosphatidylglycerol was detected. We also examined the migration activity of other phospholipids. Phosphatidylethanolamine and phosphatidylserine have migration activity, though phsophatidylchorine does not.In the present study, 1-Palmitoyl-2-was purified as a migration factor derived from omentum.