Purification of a novel migration factor for vascular smooth muscle cells, derived from omentum and its application

一种新型大网膜血管平滑肌细胞迁移因子的纯化及其应用

• 批准号: 08557059
• 负责人: SAITO Yoshihiko
• 金额: $ 8.32万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08557059/
• 关键词: omentum; migration factor of USMC; phosphadilglycerol; 大網; 血管内皮細胞; 血管新生; 内皮細胞遊走活性

Growing evidence indicates that adipose tissues have potent angiogenic activity. In the present study, we tried to purify the new angionenic factor from omentum monitoring the migration activity of vascular smooth muscle cells. Three kg of omentum was homogenized in the 15 little of methanol. After four steps of purification procedure including liquid- ; liquid partition chromatography, preparative thin-layr chromatography and two kinds of high-performance liquid chromatography, 0.3mg of purified product was obtained. Spectral analysis using fast atom bombardment mass spectrometry (FAB-MAS) ans nuclear magnetic resonance spectrometry revealed its molecular weight is 747.9 and it is 1-Palmitoyl-2-oleoyl-phosphatidylglycerol. Migration activity of synthesized 1-Palmitoyl-2-oleoyl-phosphatidylglycerol was detected. We also examined the migration activity of other phospholipids. Phosphatidylethanolamine and phosphatidylserine have migration activity, though phsophatidylchorine does not.In the present study, 1-Palmitoyl-2-was purified as a migration factor derived from omentum.

越来越多的证据表明，脂肪组织具有强大的血管生成活性。在本研究中，我们试图从网膜中纯化新的血管生成因子，以监测血管平滑肌细胞的迁移活性。将3 kg网膜在15 μ l甲醇中均质化。经液-液分配层析、制备薄层层析和两种高效液相层析四步纯化，得到0.3mg纯化产物。采用快原子轰击质谱法（FAB-MAS）和核磁共振光谱法的光谱分析表明其分子量为747.9，为1-棕榈酰-2-油酰-磷脂酰甘油。检测了合成的1-棕榈酰-2-油酰磷脂酰甘油的迁移活性。我们还检查了其他磷脂的迁移活性。磷脂酰乙醇胺和磷脂酰丝氨酸具有迁移活性，而磷脂酰胆碱不具有迁移活性。

项目成果

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