Purification of a novel migration factor for vascular smooth muscle cells, derived from omentum and its application

越来越多的证据表明，脂肪组织具有强大的血管生成活性。在本研究中，我们试图从大网膜中纯化新的血管生成因子，监测血管平滑肌细胞的迁移活性。将3 kg的网膜在15小的甲醇中均质。经过四步提纯程序，包括液体-；液相分配色谱法、制备薄层色谱法和两种高效液相色谱法，得到纯化产物0.3mg。通过快速原子轰击质谱（FAB-MAS）和核磁共振谱分析，发现其分子量为747.9，为1-棕榈酰-2-油基-磷脂酰甘油。测定了合成的1-棕榈酰-2-油酰-磷脂酰甘油的迁移活性。我们还检查了其他磷脂的迁移活性。磷脂酰乙醇胺和磷脂酰丝氨酸具有迁移活性，而磷脂酰氯则没有。在本研究中，1-棕榈酰-2被纯化为一种来自大网膜的迁移因子。

Growing evidence indicates that adipose tissues have potent angiogenic activity. In the present study, we tried to purify the new angionenic factor from omentum monitoring the migration activity of vascular smooth muscle cells. Three kg of omentum was homogenized in the 15 little of methanol. After four steps of purification procedure including liquid- ; liquid partition chromatography, preparative thin-layr chromatography and two kinds of high-performance liquid chromatography, 0.3mg of purified product was obtained. Spectral analysis using fast atom bombardment mass spectrometry (FAB-MAS) ans nuclear magnetic resonance spectrometry revealed its molecular weight is 747.9 and it is 1-Palmitoyl-2-oleoyl-phosphatidylglycerol. Migration activity of synthesized 1-Palmitoyl-2-oleoyl-phosphatidylglycerol was detected. We also examined the migration activity of other phospholipids. Phosphatidylethanolamine and phosphatidylserine have migration activity, though phsophatidylchorine does not.In the present study, 1-Palmitoyl-2-was purified as a migration factor derived from omentum.