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Purification of a novel migration factor for vascular smooth muscle cells, derived from omentum and its application

Purification of a novel migration factor for vascular smooth muscle cells, derived from omentum and its application
一种新型大网膜血管平滑肌细胞迁移因子的纯化及其应用
批准号:
08557059
负责人:
SAITO Yoshihiko
金额:
$8.32万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (A)
财政年份:
1996
资助国家:
日本
项目状态:
已结题
起止时间:
1996 至 1997

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项目成果

SAITO Yoshihiko的其他基金

相关文献

中文摘要
翻译
越来越多的证据表明,脂肪组织具有强大的血管生成活性。在本研究中,我们试图从大网膜中纯化新的血管生成因子,监测血管平滑肌细胞的迁移活性。将3 kg的网膜在15小的甲醇中均质。经过四步提纯程序,包括液体-;液相分配色谱法、制备薄层色谱法和两种高效液相色谱法,得到纯化产物0.3mg。通过快速原子轰击质谱(FAB-MAS)和核磁共振谱分析,发现其分子量为747.9,为1-棕榈酰-2-油基-磷脂酰甘油。测定了合成的1-棕榈酰-2-油酰-磷脂酰甘油的迁移活性。我们还检查了其他磷脂的迁移活性。磷脂酰乙醇胺和磷脂酰丝氨酸具有迁移活性,而磷脂酰氯则没有。在本研究中,1-棕榈酰-2被纯化为一种来自大网膜的迁移因子。
英文摘要
Growing evidence indicates that adipose tissues have potent angiogenic activity. In the present study, we tried to purify the new angionenic factor from omentum monitoring the migration activity of vascular smooth muscle cells. Three kg of omentum was homogenized in the 15 little of methanol. After four steps of purification procedure including liquid- ; liquid partition chromatography, preparative thin-layr chromatography and two kinds of high-performance liquid chromatography, 0.3mg of purified product was obtained. Spectral analysis using fast atom bombardment mass spectrometry (FAB-MAS) ans nuclear magnetic resonance spectrometry revealed its molecular weight is 747.9 and it is 1-Palmitoyl-2-oleoyl-phosphatidylglycerol. Migration activity of synthesized 1-Palmitoyl-2-oleoyl-phosphatidylglycerol was detected. We also examined the migration activity of other phospholipids. Phosphatidylethanolamine and phosphatidylserine have migration activity, though phsophatidylchorine does not.In the present study, 1-Palmitoyl-2-was purified as a migration factor derived from omentum.
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会议论文
M.Yoshibayashi et.al.: "Elevated levels of adrenomedullin in primary and secondary pulmonary hypertension in patients <20 years of age." Am J Cardiol. 79. 1556-1556 (1997)
M.Yoshibayashi 等人:“<20 岁的原发性和继发性肺动脉高压患者的肾上腺髓质素水平升高。”
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T.Nishikimi et.al.: "Relationship between left ventricular geometry and natriuretic peptide levels in essential hypertension." Hypertension. 28(1). 22-30 (1996)
T.Nishikimi 等人:“原发性高血压中左心室几何形状与利尿钠肽水平之间的关系。”
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