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Development of insulin secreting B cell line for Diabetes Mellitus by transgenic pigs

通过转基因猪开发治疗糖尿病的胰岛素分泌 B 细胞系

基本信息

批准号：
08557071
负责人：
INOUE Kazutomo
金额：
$ 10.82万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

Major field of our research is pancreatic islet transplantation. Pancreatic islet transplantation could be alternative of curative therapy to the patient with type I diabetes mellitus. To date, clinical application of islet allo-transplantation has not carried out in Japan. We are going to establish the system prepared for clinical islet allo-transplantation. The system involves two specialized techniques that have been considered essential for successful transplantation. One is islet isolation technique and other is cryopreservation technique of isolated islet for islet bank.One of the crucial problems on clinical islet allo-transplantation is the control of quantity and quality of isolated islets during isolation processes that may affect the function of grafted islets We have previously reported the method for islet isolation with excellent results on large-scale animal model. Other technique we have explored for clinical application of islet allo-transplantation regards cryopreserv … More ation of isolated islet. Recently, we have developed the method for cryopreservation of isolated islet that is important for the establishment of islet banking system. Establishment of islet banking system would contribute to solve the serious problems of shortage of donor islet. For the complete evaluation of our method, further studies using human pancreas are needed at our institution. We have applied the research protocol using human pancreas to the institutional committee board of Kyoto University. After the approval of the research protocol using human pancreas, we will immediately carry the study on for clinical application. We have various themes on experimental islet transplantation such as isolation of xeno-porcine islet, bioartificial pancreas, pancreatic beta cell line, mechanisms regarding immune-responses on xeno-transplantation and site for islet transplantation. We have supposed the strategy to establish the method of pancreatic islet transplantation employing bioartificial pancreas encapsulating xeno-porcine islets and or pancreatic beta cells as a curative therapy for the patient with type I diabetes mellitus. We would like to conduct our research on pancreatic islet transplantation with multiple view points in future. Less

我们的主要研究领域是胰岛移植。胰岛移植可作为I型糖尿病治疗的一种选择。迄今为止，胰岛同种异体移植的临床应用尚未在日本进行。本研究旨在建立适合临床应用的同种异体胰岛移植系统。该系统涉及两种被认为是成功移植必不可少的专业技术。一是胰岛分离技术，二是胰岛库分离胰岛的冷冻保存技术。临床同种异体胰岛移植的关键问题之一是分离过程中分离胰岛的数量和质量的控制，这可能会影响移植胰岛的功能。我们之前已经报道了胰岛分离的方法，在大规模动物模型上取得了良好的效果。本研究还探索了胰岛移植临床应用的其他技术，如冷冻保存、 ...更多信息分离的胰岛。近年来，我们成功地建立了胰岛的冷冻保存方法，这对胰岛库的建立具有重要意义。建立胰岛银行系统将有助于解决胰岛供体短缺的严重问题。为了对我们的方法进行全面评价，我们需要在我们的机构中使用人类胰腺进行进一步研究。我们已经将使用人类胰腺的研究协议应用于京都大学的机构委员会。在使用人胰腺的研究方案获得批准后，我们将立即进行临床应用研究。我们有各种主题的实验胰岛移植，如异种猪胰岛的分离，生物人工胰腺，胰岛β细胞系，异种移植的免疫反应机制和胰岛移植部位。我们提出了建立一种以异种猪胰岛和/或胰岛β细胞为载体的生物人工胰腺胰岛移植治疗I型糖尿病的策略。我们希望今后能从多个角度对胰岛移植进行研究。少