DEVELOPMENT OF MMONITERING SYSTEM OF MUTAGENS IN ENVIRONMENTAL SAMPLE

各种生物测定法已被应用于评价环境水和处理水的安全性。umu试验是一种利用鼠伤寒沙门氏菌SOS功能检测遗传毒性的方法。umu试验的方案分为5个阶段：（1）试验菌株的预孵育，（2）样品的加入，（3）umuC基因的诱导，（4）β-半乳糖苷酶酶反应，（5）分光光度法测定吸收值并计算活性。在通常的程序中，在本研究中，我们尝试发展流动注射式的装置，以自动化的方式进行umu测试。使培养物流过管并通过使用旋转泵添加样品。对AF-2的遗传毒性检测条件进行了研究和改进。在1.5小时内，试验菌株在36 ℃时活性最高，在2.0小时以上，在37 ℃时活性最高。在每个阶段，我们还研究了一些条件，我们建议新的流动注射方式的设计可以有效地筛选或监测环境水的遗传毒性。

Various bioassays have been applied to evaluate safety of environmental water and treated water. The umu-test is a method for the detection of genotoxicity by using SOS function of Salmonella typhimurium.The protocol of the umu-test in devided into 5 phases, (1) pre-incubation of test strain, (2) addition of samples, (3) induction of umuC gene, (4) Beta-galactosidase enzymati reaction and (5) measurement of absorption in a spectrophotometry and calculation of the activity.In usual procedure, all phases are performed by batch type reactors, using test tubes.In this study, we tried to developed the device of flow-injection style to perform the umu test automatically. The culture was flowed through a tube and added samples by using a rotary pump. The various conditions were investigated and modified for detection of genotoxocity of AF-2.Even after 24hr of incubation under control, test strain showed activity. Within 1.5hrs, the test strain was most active at 36 deg.C and longer than 2.0hrs, it was so at 37 deg.C.At each phases some more conditions have been investigated, and we suggest that new devise of flow-injection style could be effective to screen or monitor the genotoxicity of environmental water.