DEVELOPMENT OF MMONITERING SYSTEM OF MUTAGENS IN ENVIRONMENTAL SAMPLE

各种生物测定法已被应用于环境水和处理水的安全性评价。umu试验是一种利用SOS功能检测鼠伤寒沙门菌遗传毒性的方法。umu试验方案分为5个阶段：(1)试验菌株的预孵育，(2)样品的加入，(3)umuC基因的诱导，(4)β -半乳糖苷酶反应，(5)分光光度法测定吸收并计算活性。在通常的程序中，所有的相都是在批式反应器中使用试管进行的。在本研究中，我们尝试开发流动注射式的装置来自动进行umu测试。培养液流过管子，用旋转泵加入样品。对各种检测AF-2基因毒性的条件进行了研究和改进。即使在控制下孵育24小时后，试验菌株仍显示出活性。在1.5h内，试验菌株在36℃时活性最强，在2.0h以上时活性最强，在37℃时活性最强。在每个阶段都进行了一些条件的研究，我们认为新的流动注射式设计可以有效地筛选或监测环境水的遗传毒性。

Various bioassays have been applied to evaluate safety of environmental water and treated water. The umu-test is a method for the detection of genotoxicity by using SOS function of Salmonella typhimurium.The protocol of the umu-test in devided into 5 phases, (1) pre-incubation of test strain, (2) addition of samples, (3) induction of umuC gene, (4) Beta-galactosidase enzymati reaction and (5) measurement of absorption in a spectrophotometry and calculation of the activity.In usual procedure, all phases are performed by batch type reactors, using test tubes.In this study, we tried to developed the device of flow-injection style to perform the umu test automatically. The culture was flowed through a tube and added samples by using a rotary pump. The various conditions were investigated and modified for detection of genotoxocity of AF-2.Even after 24hr of incubation under control, test strain showed activity. Within 1.5hrs, the test strain was most active at 36 deg.C and longer than 2.0hrs, it was so at 37 deg.C.At each phases some more conditions have been investigated, and we suggest that new devise of flow-injection style could be effective to screen or monitor the genotoxicity of environmental water.