DEVELOPMENT OF MMONITERING SYSTEM OF MUTAGENS IN ENVIRONMENTAL SAMPLE

各种生物检测方法已被应用于评价环境水和经处理的水的安全性。Umu试验是利用鼠伤寒沙门氏菌的SOS功能进行遗传毒性检测的一种方法。umu试验分为5个阶段：(1)试验菌株的预孵育，(2)样品的加入，(3)UMUC基因的诱导，(4)β-半乳糖苷酶反应和(5)吸光度的测定和活性的计算。在通常的步骤中，所有阶段都由间歇式反应器进行，使用测试管。本研究试图开发流动注射式的装置来自动进行UMU试验。培养物通过试管流动，并使用旋转泵添加样本。对检测AF-2基因毒力的各种条件进行了考察和改进，即使在对照条件下培养24小时后，试验菌株仍显示出活性。在1.5小时内，受试菌在36℃下达到最高活性，并持续超过2.0小时，在37℃下达到最高活性。在每个阶段都进行了更多的条件考察，我们认为新的流动注射方式可以有效地筛查或监测环境水的遗传毒性。

Various bioassays have been applied to evaluate safety of environmental water and treated water. The umu-test is a method for the detection of genotoxicity by using SOS function of Salmonella typhimurium.The protocol of the umu-test in devided into 5 phases, (1) pre-incubation of test strain, (2) addition of samples, (3) induction of umuC gene, (4) Beta-galactosidase enzymati reaction and (5) measurement of absorption in a spectrophotometry and calculation of the activity.In usual procedure, all phases are performed by batch type reactors, using test tubes.In this study, we tried to developed the device of flow-injection style to perform the umu test automatically. The culture was flowed through a tube and added samples by using a rotary pump. The various conditions were investigated and modified for detection of genotoxocity of AF-2.Even after 24hr of incubation under control, test strain showed activity. Within 1.5hrs, the test strain was most active at 36 deg.C and longer than 2.0hrs, it was so at 37 deg.C.At each phases some more conditions have been investigated, and we suggest that new devise of flow-injection style could be effective to screen or monitor the genotoxicity of environmental water.