Study on expression system of C4 photosynthetic genes.

C4光合基因表达系统的研究。

• 批准号: 08640824
• 负责人: MATSUOKA Makoto
• 金额: $ 1.6万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640824/
• 关键词: C4 photosynthesis; Pyruvate orthosphate dikinase; Phosphoenolpyruvate carboxylase; Gene evolution; Gene expression; C_4光合成遺伝子; ホスホエノールピルビン酸Piジキナーゼ; C_4光合成; ピルビン酸Piジキナーゼ; プロモーター解析

C3 plants including many agronomically important crops exhibit a lower photosynthetic efficiency due to inhibition of photosynthesis by O_2 and the associated photorespiration. C4 plants had evolved the C4 pathway to overcome low CO_2 and photorespiration. This review first focuses on the generation of a system for high level expression of the C4-specific gene for pyruvate, orthophosphate dikinase (Pdk), one of the key enzyme in C4 photosynthesis. Based on the results with transgenic rice plants, we have demonstrated that the regulatory system controlling the Pdk expression in maize is not unique to C4 plants but rice (C3 plant) posses a similar system. Second, we discuss the possibility of the high level expression of maize C4-specific genes in transgenic rice plants Introduction of the maize intact phosphoenolpyruvate carboxylase gene (Ppc) caused 30-100 fold higher PEPC activities than non-transgenic rice. These results demonstrated that intact C4-type genes are available for high level expression of C4 enzymes in rice plants.

C3植物包括许多重要的农艺作物，由于O_2对光合作用和相关光呼吸的抑制而表现出较低的光合效率。C4植物已经进化出C4途径来克服低CO_2和光呼吸。本文首先综述了C4光合作用的关键酶之一——丙酮酸正磷酸二激酶（Pdk）的高水平表达体系的建立。基于转基因水稻的结果，我们已经证明控制Pdk在玉米中的表达的调控系统不是C4植物所独有的，水稻（C3植物）也具有类似的系统。其次，我们讨论了玉米c4特异性基因在转基因水稻中高表达的可能性，引入玉米完整磷酸烯醇丙酮酸羧化酶基因（Ppc）后，PEPC活性比非转基因水稻高30-100倍。这些结果表明，完整的C4型基因可用于水稻C4酶的高水平表达。

项目成果

Uchino, A., Nemoto, K., Ishii, R., Samejima, M.and Matsuoka, M.: "C4-type gene expression is not directly dependent on Kranz anatomy in an amphibious sedge, Eleocharis vivipara Link." Plant J. (in press). (1997)

Uchino, A.、Nemoto, K.、Ishii, R.、Samejima, M. 和 Matsuoka, M.：“C4 型基因表达并不直接依赖于两栖莎草 Eleocharis vivipara Link 中的 Kranz 解剖结构。”

Imaizumi,Nら: "Characterization of gene for pyruvate,orthophosphate dikinase from the C_3 plant rice and comparison of structure and expression between C_3 and C_4 ppdk genes." Plaut Mol.Biol.(印刷中). (1997)

Imaizumi, N 等人：“C_3 植物水稻丙酮酸、正磷酸二激酶基因的表征以及 C_3 和 C_4 ppdk 基因的结构和表达比较”（Plaut Mol.Biol）。

野村 美加 et.al.: "化学と生物" 学会出版センター, 5 (1998)

Mika Nomura 等人：《化学与生物学》学会出版中心，5 (1998)

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Gallusci，P. 等人：“发育中的玉米胚乳中胞质丙酮酸、正磷酸二激酶表达的调节”植物分子生物学 31. 45-55 (1996)

Sanmiya,Kら: "Cloning of a cDNA that encodes farresyl diphosphate sythetase and the blue lightinduced expression of the corresponding gene in the leaves of rice plants." Biochim.Biophys.Acta. (印刷中). (1997)

Sanmiya, K 等人：“编码法雷基二磷酸合成酶的 cDNA 的克隆以及蓝光诱导水稻叶片中相应基因的表达”（1997 年出版）。