Functional analysis of KN1-type homeodomain protein

KN1型同源域蛋白的功能分析

• 批准号: 12440227
• 负责人: MATSUOKA Makoto
• 金额: $ 7.87万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12440227/
• 关键词: homeobox gene; rice; tobacco; gibberellin (GA); transgenic plant; 形質転換植物; ホメオドメイン; ジベレリン; 転写制御

KNOX horaeodomain proteins are encoded by knottedl-like homeobox (knox) genes that constitute a gene family in plants. Similar to the animal homeodomain proteins, KNOX proteins are consider ed to be key transcriptional regulators that control the expression of genes involved in plant organogenesis at the shoot apical meristemi Therefore, in order to understand the developmental processes in plants, it is important to elucidate the molecular mechanisms underlying KNOX pro tein regulation of gene expression. In thip study, we investigated the structural features and the mechanisms for expression of KNOX proteins. Through the experiments with transgenic plants, a KNOX protein, OSH15, directly interacts with the specific sequence of intron 1 in the gene e needing GA biosynthetic enzyme, GAC20 oxidase and suppresses its expression.

KNOX horaeodomain蛋白由植物中构成基因家族的knottedl-like homeobox（knox）基因编码。与动物同源结构域蛋白类似，KNOX蛋白被认为是植物茎尖分生组织器官发生相关基因表达的关键转录调控因子。因此，为了了解植物的发育过程，阐明KNOX蛋白调控基因表达的分子机制是非常重要的。在本研究中，我们研究了KNOX蛋白的结构特征和表达机制。通过对转基因植物的实验，发现KNOX蛋白OSH 15直接与GA生物合成酶GAC 20氧化酶基因中内含子1的特异序列相互作用，抑制其表达。

项目成果

Nishimura, A: "Over-expression of tobacco knottedl-type classl homeobox genes alters various leaf morphology"Plant Cell Physiol. 41. 583-590 (2000)

Nishimura，A：“烟草结型1类同源盒基因的过度表达改变了各种叶子形态”植物细胞生理学。

Kikuchi, K: "Molecular analysis of the NAG gene family in rice"Mol. Gen. Genet. 262. 1047-1051 (2000)

Kikuchi, K：“水稻 NAG 基因家族的分子分析”Mol。

Ito, M.: "Involvement of homeobox genes in early body plan of monocot."International review of cytology(in press).

Ito, M.：“单子叶植物早期身体规划中同源框基因的参与。”国际细胞学评论（出版中）。

Itoh,J.-I.: "SHOOT ORGANIZATION genes regulate shoot apical meristem organization and the pattern of leaf primordium initiation in rice."Plant Cell. 12. 2161-2174 (2000)

Itoh, J.-I.：“芽组织基因调节水稻芽顶端分生组织和叶原基起始的模式。”《植物细胞》。

Ito, M: "Involvement of homeobox genes in early body plan of monocot"International review of cytology. in press. (2001)

Ito, M：“单子叶植物早期身体规划中同源盒基因的参与”国际细胞学评论。