Study on gibberellin perception

赤霉素感知研究

• 批准号: 18107001
• 负责人: MATSUOKA Makoto
• 金额: $ 71.55万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18107001/
• 关键词: 成長生理; 植物ホルモン; 遣伝子; シグナル伝達; 発現制御; 遺伝子

We analyzed the 3D structure of rice GA receptor (OsGID1) bound with GA. The overall structure shows an α/β-hydrolase fold similar to that of HSLs. The GA-binding pocket corresponds to the substrate-binding site of HSLs. On the basis of the OsGID1 structure, we mutagenized important residues for GA binding and examined their binding activities. Almost all of them showed very little or no activity, confirming that the residues revealed by structural analysis are important for GA binding. The observations indicate that GID1 originated from HSL and was further modified to have higher affinity and more strict selectivity for bioactive GAs by adapting the amino acids involved in GA binding in the course of plant evolution.

我们分析了与 GA 结合的水稻 GA 受体 (OsGID1) 的 3D 结构。整体结构显示出与 HSL 相似的 α/β-水解酶折叠。 GA 结合口袋对应于 HSL 的底物结合位点。在OsGID1结构的基础上，我们对GA结合的重要残基进行诱变并检查其结合活性。几乎所有这些都显示出很少或没有活性，证实结构分析揭示的残基对于 GA 结合很重要。观察结果表明，GID1 起源于 HSL，并通过在植物进化过程中调整参与 GA 结合的氨基酸，进一步修饰为对生物活性 GA 具有更高的亲和力和更严格的选择性。

项目成果

Identification and characterization of Arabidopsis gibberellin rexeptor.

拟南芥赤霉素受体的鉴定和表征。

• 发表时间: 2006
• 期刊: Plant J. 46
• 作者: Nakajima;M.
• 通讯作者: M.

Gibberellin porception and signal transduction in rice.

水稻中赤霉素的感知和信号转导。

• 发表时间: 2007
• 作者: Ueguchi-Tanaka;M.
• 通讯作者: M.

The rice SPINDLY gene functions as a negative regulator of gibberellin signaling by controlling the suppressive function of the DELLA protein, SLR1, and modulating brassinosteroid synthesis

• DOI: 10.1111/j.1365-313x.2006.02875.x
• 发表时间: 2006-11-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Shimada, Asako;Ueguchi-Tanaka, Miyako;Matsuoka, Makoto
• 通讯作者: Matsuoka, Makoto

A soluble gibberellin receptor, GID1, and early gibberellin signal transduction

可溶性赤霉素受体、GID1 和早期赤霉素信号转导

• 发表时间: 2007
• 作者: Makoto;M.;Hirano;K. and Ueguchi-Tanaka;M
• 通讯作者: M

Highly Sensitive and High-Throughput Analysis of Plant Hormones Using MS-Probe Modification and Liquid ChromatographyTandem Mass Spectrometry: An Application for Hormone Profiling in Oryza sativa

• DOI: 10.1093/pcp/pcp057
• 发表时间: 2009-07-01
• 期刊: PLANT AND CELL PHYSIOLOGY
• 影响因子: 4.9
• 作者: Kojima, Mikiko;Kamada-Nobusada, Tomoe;Sakakibara, Hitoshi
• 通讯作者: Sakakibara, Hitoshi