Evaluation of oocytes activation by oocyte-ativating factors derived from sperm extract.

精子提取物中的卵母细胞激活因子对卵母细胞激活的评估。

• 批准号: 08660352
• 负责人: HOSOI Yoshihiko
• 金额: $ 1.41万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660352/
• 关键词: microfertilization; pronucleous formation; oocyte activating factor; 顕微受精

In mammalian oocytes, fertilization-associated calcium [Ca2+]i oscillations are responsible for the activation of development. It has been proposed that the sperm may interact with receptors in the oocyte's plasma membrane and engage intracellular signaling pathways that result in Ca2+ release. A different line of investigation suggests that upon sperm-oocyte fusion, a sperm cytosolic factor is released into the oocyte which interacts with unknown cytosolic targets, and generates [Ca2+]i oscillations. In this study, we demonstrated that injections of xeno-sperm crude extracts (SF) into other mammalian oocytes couses activation and in vitro parthenogenetic development. A hypothesis we supported in the mechanism of signal transduction at fertilization proposes that a factor from the sperm (SF) is released into the egg and, by interacting with unknown cytosolic targets, generates activation of oocytes. Injection of various sperm into hamster, mouse, rabbit, pig and bovine oocytes (this me … More ans that gametes' membrane fusion was by-passed) activated oocytes similar to those observed during fertilization, in our study. To further test the physiological relevance of SF,SF is obtained from porcine sperm were injected into hamster oocytes. It was caused pronuclear formation but formation is delayd compared to that of normal hamster fertilization. Probably it was due to fertilized hamster oocytes oscillate with higher frequency than porcine oocytes. Thus, if SF contains a physiological initiator of [Ca2+]i oscillations, injection of SF from one species into oocytes of a different species should be able to induce Ca2+ responses in these oocytes and it should respect the pattern characteristic of each species. Long lasting oscillations are characteristic of mammalian fertilization and they are critical for the activation of embryonic development. It may triggered by sperm cytosolic factors. For example, stimulation of this pathway may be used for parthenogenetic activation in embryo cloning and its inhibition may offer insights in contraception. Less

在哺乳动物卵母细胞中，受精相关的钙[Ca 2 +]i振荡负责激活发育。有人提出精子可能与卵母细胞质膜上的受体相互作用，并参与导致Ca 2+释放的细胞内信号传导途径。另一种研究表明，精子-卵母细胞融合后，精子胞质因子被释放到卵母细胞中，与未知的胞质靶点相互作用，并产生[Ca 2 +]i振荡。本研究证明，将异种精子粗提物（SF）注射到其他哺乳动物卵母细胞中可引起激活和体外孤雌发育。我们在受精信号转导机制中支持的一个假设提出，精子中的一种因子（SF）被释放到卵子中，通过与未知的胞质靶点相互作用，产生卵母细胞的激活。将各种精子注射到仓鼠、小鼠、兔、猪和牛的卵母细胞中（本实验用的是精子注射器）， ...更多信息 在我们的研究中，配子的膜融合被绕过）激活的卵母细胞与受精过程中观察到的相似。为了进一步测试SF的生理相关性，从将猪精子注射到仓鼠卵母细胞中获得SF。它引起原核形成，但与正常仓鼠受精相比，形成延迟。这可能是由于受精后仓鼠卵母细胞的振荡频率高于猪卵母细胞。因此，如果SF含有[Ca 2 +]i振荡的生理引发剂，则将SF从一个物种注射到不同物种的卵母细胞中应该能够诱导这些卵母细胞中的Ca 2+反应，并且应该尊重每个物种的模式特征。长时间的振荡是哺乳动物受精的特征，它们对激活胚胎发育至关重要。它可能由精子胞浆因子触发。例如，刺激该途径可用于胚胎克隆中的孤雌激活，而抑制该途径可为避孕提供见解。少

项目成果

Y.Hosoi: "Microinjection of domestic and zoo animalspermatozoa into oocytes and their development." Zygote. (in press). (1998)

Y.Hosoi：“将家畜和动物园动物的精子显微注射到卵母细胞中及其发育。”

Y.Hosoi: "Evaluation of oocytes activating activity by porcine sperm extract." Jpn.J.Embryo Trans.(in print). (1998)

Y.Hosoi：“猪精子提取物对卵母细胞激活活性的评估。”

Y.Hosoi: "Fertilization in vitro by microinjection of sperm head in rabbit and cattle." Mem.School B.O.S.T.Kinki Univ.1. 59-65 (1997)

Y.Hosoi：“通过显微注射兔子和牛的精子头进行体外受精。”

Y.Hosoi: "Microinjection of domestic and zoo animal spermato zoa into oocytes and their development" Zygote. (in print). (1998)

Y.Hosoi：“将家畜和动物园动物精子显微注射到卵母细胞中及其发育”受精卵。

Y.Hosoi: "Reproductive Biology Update" H.Miyamoto & N.Manabe, 498 (1998)

Y.Hosoi：“生殖生物学更新” H.Miyamoto