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EMBRYO PRODUCTION MODEL OF ENDANGERED SPECIES IN BOVIOIDEA BY IN VITRO FERTILIZATION USING SPERM INJECTION

牛科濒危物种精子注射体外受精胚胎生产模型

基本信息

批准号：
06660393
负责人：
HOSOI Yoshihiko
金额：
$ 1.22万
依托单位：
KINKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Conservation of non-domestic endangered species as genetic resource is an important subject of animal science. Recent advances in the micro-manipulation techniques and accumulation of the fundamental research achievement will greatly contribute to this subject. Main purpose of this research is to produce embryos by microinjection of spermatozoa derived from dead animals. Dead animals belonged to bovioidea were offered by Osaka City Zoo. Spermatozoa were recovered from frozen-thawed testis or epididymis. Condition of these materials was not stable. Surviving rate of micro-assisted fertilization oocytes depended on the method of injection of spermatozoa. We got a few samples of normal xenon-fertilized oocytes. This esult proved to use this system for embryo production, however still too low to use it practically. Moreover, supply of matured oocytes for fertilization become critical for production of embryos. According to our data, sperm derived from dead animals will be able to fertilize oocytes by assisted fertilization soon. However, we can recover eligible oocytes for in vitro fertilization in the ovary derived from dead animals. Further research effort is necessary for development of maturing immature oocytes without meiotic competence. In conclusion, the potential for the development of these techniques for use in animal breeding and conservation of endangered species is clearly very great.

作为遗传资源的非家养濒危物种的保护是动物科学的一个重要课题。微操纵技术的最新进展和基础研究成果的积累将为这一学科的发展做出巨大贡献。本研究的主要目的是通过动物死亡精子的显微注射产生胚胎。大阪市动物园提供了属于牛科的动物尸体。精子从冷冻解冻的睾丸或附睾中恢复。这些材料的状态不稳定。微辅助受精卵母细胞的成活率取决于精子注射方式。我们有一些正常的氙受精卵母细胞样本。这一结果证明了该系统可用于胚胎生产，但仍不足以实际应用。此外，为受精提供成熟的卵母细胞对胚胎的产生至关重要。根据我们的数据，从死亡动物身上提取的精子很快就能通过辅助受精使卵母细胞受精。然而，我们可以从死亡动物的卵巢中恢复适合体外受精的卵母细胞。对于没有减数分裂能力的成熟未成熟卵母细胞的发育需要进一步的研究。总之，这些技术在动物繁殖和濒危物种保护方面的发展潜力显然是巨大的。