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犬バベシア感染にともなう赤血球膜の酸化傷害と貧血

与犬巴贝虫感染相关的红细胞膜氧化损伤和贫血

基本信息

批准号：
08660385
负责人：
MAKIMURA Susumu
金额：
$ 1.47万
依托单位：
MIYAZAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660385/
关键词：
Babesia gibsoni infection dog anemia RBC-bound IgG commpetitive ELISA Band 3 oxidative damage Babesia gibsoni injection RBC-bound IgG oxidative damage Babesia gibsoni anepua RBC-binding IgG Competitute ELISA

项目摘要

Anemia is the primary clinical finding manifested by Babesia gibsoni-infected dogs. Significant elevated anti-erythrocyte ELISA levels were demonstrated in sera of infected dogs. To clarify the involvement of anti-erythrocyte IgG antibody in anemia during B.gibsoni infection, a simple method to quantify erythrocyte surface-bound IgG was established and applied to quantification of bound IgG in infected and healthy dogs. Erythrocyte surface-bound IgG value was shown to elevate significantly in clinical infected dogs compared to that of subclinically infected and healthy dogs, moreover the increased level was consistent with Coombs'test results. Serum anti-RBCm antibodies of the dogs before and after the experimental inoculation with parasitized erythrocytes reacted with cytoskeletal and transmembrane structural (band 3) proteins of either self or non-self RBCm. Anti-RBCm antibodies eluted from erythrocytes of B.gibsoni-infected dogs reacted with band 3 and 4.2 of self and non-self RBCm. Anti-RBCm and the surface of RBC treated with phenylhydrazine or neuraminidase, but not to that of non-treated treated RBC.Based from this results, anti-cytoskeletal protein antibody in sera of B.gibsoni-infected dogs is considered to attach to native RBCm, and furthermore free radical-induced oxidative stress imposed on RBC and sialic acid removal from grycoproteins of RBC are considered necessary for anti-transmembrane protein antibody in sera of B.gibsoni-infected dogs to be bound to RBCs. These are important to elucidating the mechanism of the marked in crease in RBCs-bound IgG value and anemia in B.gibsoni infection.

贫血是吉氏巴贝虫感染犬的主要临床表现。受感染狗的血清中抗红细胞 ELISA 水平显着升高。为了阐明抗红细胞 IgG 抗体在吉布氏博虫感染期间贫血中的作用，建立了一种简单的定量红细胞表面结合 IgG 的方法，并将其应用于感染和健康狗中结合 IgG 的定量。与亚临床感染犬和健康犬相比，临床感染犬的红细胞表面结合IgG值显着升高，而且升高的水平与Coombs试验结果一致。在实验性接种寄生红细胞之前和之后，狗的血清抗RBCm抗体与自体或非自体RBCm的细胞骨架和跨膜结构（带3）蛋白反应。从感染吉布氏杆菌的狗的红细胞中洗脱的抗RBCm抗体与自身和非自身RBCm的条带3和4.2反应。抗RBCm和用苯肼或神经氨酸酶处理的红细胞表面，但不与未处理的处理的红细胞相比。基于该结果，B.gibsoni感染的狗血清中的抗细胞骨架蛋白抗体被认为附着于天然RBCm，此外自由基诱导的氧化应激对红细胞施加和唾液酸去除来自红细胞的糖蛋白被认为是吉布氏伯克氏菌感染狗血清中的抗跨膜蛋白抗体与红细胞结合所必需的。这些对于阐明吉布氏博氏菌感染中红细胞结合IgG值显着升高和贫血的机制具有重要意义。