Mechanism of Bone Growth in Length-Vascular Invasion and Formation of Chondroclast

长度方向的骨生长机制-血管侵袭和破软骨细胞形成

• 批准号: 08670037
• 负责人: FUKUSHIMA Osamu
• 金额: $ 0.83万
• 依托单位: Jikei university School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670037/
• 关键词: GROWTH PLATH; ENCHONDRAL OSSIFICATION; CHONDROCLAST; HISTOCHEMISTRY; PROTEASE; ELECTRON MICROSCOPE

The present study was planned for investigating mechanisms of bone growth in length. The author employed 3-week old chicken because they showed longer vascular invasion in the growth plate than that in the mammmalia. This is a benefit for observation with electron microscope.Serial Epon simithin sections showed the looped capillary at the tip of vascular invasion. There was no cartilage resorbing cell. The peptidase and lysosomal enzyme histochemical results also supported it. Multinuclear large cells showing small vesicles in the cytoplasm and no ruffled border were seen in the peri-vascular space at vascular invasion lower than the tip. An alkaline pnitrophenylphosphatase activity was positive on the small vesicle membrane, resulted that they were a kind of chondroclasts. At further lower portion, attached chondroclasts were observed on the surface of the cartilage matrix. There, a mono-nuclear prechondroclast fuesd to an attached chondroclast. The author identified there with "chondroclast formation site".From these findings, the author concluded that a chondroclasts was yielded at the chondroclast formation site, and moved in the peri-vascular space toward to the tip as a moving chondroclast when the bone grew in length.

本研究旨在研究骨长度生长的机制。作者选用了3周龄的鸡，因为它们在生长板中的血管侵入比在睾丸中长。Epon连续切片显示血管浸润尖端有毛细血管环。无软骨吸收细胞。肽酶和溶酶体酶组化结果也支持这一观点。在低于尖端的血管浸润处的血管周围可见多核大细胞，胞质内有小泡，无皱褶边缘。小泡膜上碱性磷酸酶呈阳性，表明它们是一种破软骨细胞。在更低的部分，在软骨基质的表面上观察到附着的破软骨细胞。在那里，单核前软骨细胞融合到附着的软骨细胞上。作者将其确定为“破软骨细胞形成部位”。根据这些发现，作者得出结论，在破软骨细胞形成部位产生了破软骨细胞，当骨长度增长时，破软骨细胞在血管周围间隙中向尖端移动，成为移动的破软骨细胞。

项目成果

Fukushima, O.: "Enzyme histochemistry of hard tissues : Proteases activity in chicken bone tissues" Acta Histochemica et Cytochemica. 29-Suppl. 382-383 (1996)

Fukushima, O.：“硬组织的酶组织化学：鸡骨组织中的蛋白酶活性”Acta Histochemica et Cytochemica。

Sakai, T.: "The covering method : An improved negative staining method for ultrathin Cryo-sections of tissues" Journal of Electron Microscopy. 44・6. 479-484 (1995)

Sakai，T.：“覆盖方法：一种改进的组织超薄冷冻切片的负染色方法”《电子显微镜杂志》44・6（1995）。

Tanaka, H.: "Phenotypic characteristics and significance of reticular meshwork surrounding splenic white pulp of mice." Journal of Electron Microscopy. 45-5. 407-416 (1996)

Tanaka, H.：“小鼠脾白髓周围网状网络的表型特征和意义。”

Tanaka, H.: "Phenotypic characteristics and Significance of reticular meshwork Surrounding splenic white pulp of mice" Journal of Electron Microscopy. 45・5. 407-416 (1996)

Tanaka, H.：“小鼠脾白髓周围网状网络的表型特征和意义”，电子显微镜杂志 45・5（1996）。

Fukushima,O.: "Enzyme histochemistry of hard tissues : Proteases activity in chicken bone tissues" Acta Histochemica et Cytochemica. 29 (Suppl). 382-383 (1996)

Fukushima,O.：“硬组织的酶组织化学：鸡骨组织中的蛋白酶活性”Acta Histochemica et Cytochemica。