Interactions between Schwann cell and axon during reinnervation to the motor endplate

运动终板神经再支配过程中雪旺细胞和轴突之间的相互作用

• 批准号: 08670020
• 负责人: KAWABUCHI Masaru
• 金额: $ 1.41万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670020/
• 关键词: Neuromuscular junction; Axonal guidance; Neural regeneration; Aging; Immunohistochemistry; Schwann cell; Extracellular material; Adhesion molecule; 加齡

Age-related changes of the interactions between the axon, Schwann cell and extracellular material on reinnervation to the motor endplate were examined during the period of I-8 weeks after crush injury of the sciatic nerve with immunonuofluorescence histochemistry. Observations were done by confocal laser scanning microscopy. To define the distribution and co-localization of the structural components of the regenerating nerve fibers, some specific antibodies were used to label axons (neurofilament, PGP 9.5), Schwann cells (S 100), adhesion molecules [neural cell adhesion molecule. (N-CAM)] and the extracellular material (tenascin). In addition, the structural associations between regenerating nerves and acetylcholine receptors (AChR) were also examined. In the aged animal, the main abnormalities in the process of reinnervation consisted of occasional preterminal and terminal axons which either took a distorted course or were damaged. The most obvious changes was the consistency with degeneration of the terminal Schwann cells. In the motor endplate, loci of aneural or abandoned AChR were frequently found particularly late in the reinnervation process. The distribution and continuities of N-CAM-like immunoreactivity around the Schwann cells Were deranged, and the tenascin pathway through which regenerating axons pass was incompletely constructed. These results taken together indicate disturbance in the axonal guidance coupled with incomplete reformation of synapses with a decrease in the effective area of synaptic contact. This may be due to an age-related unsynchronous maturation among the Schwann cells, axon, and extracellular material during the nerve regeneration.

应用免疫荧光组织化学方法观察坐骨神经挤压损伤后1 ~ 8周，轴突、雪旺细胞和细胞外物质相互作用对运动终板再神经支配的年龄相关性变化。用激光共聚焦扫描显微镜观察。为了确定再生神经纤维结构成分的分布和共定位，使用一些特异性抗体标记轴突（神经丝，PGP 9.5），雪旺细胞（S 100），粘附分子[神经细胞粘附分子]。（N-CAM）]和细胞外物质（tenascin）。此外，还研究了再生神经与乙酰胆碱受体（AChR）之间的结构关联。老龄动物再神经支配过程中主要异常为偶有前、终轴突的畸变或损伤。最明显的变化是终末雪旺细胞变性的一致性。在运动终板中，经常发现神经或废弃的AChR位点，特别是在神经再支配过程的晚期。n - cam样免疫反应性在雪旺细胞周围的分布和连续性紊乱，再生轴突通过的腱素通路不完全构建。综上所述，这些结果表明轴突引导受到干扰，突触重组不完全，突触有效接触面积减少。这可能是由于在神经再生过程中，雪旺细胞、轴突和细胞外物质之间存在与年龄相关的不同步成熟。

项目成果

Masaru Kawabuchi: "The effect of aging on the morphological nerve changes during nerve regeneration" Restorative Neruorolgy & Neruoscience. (accepted).

Masaru Kawabuchi：“衰老对神经再生过程中神经形态变化的影响”恢复神经学

Masaru Kawabuchi: "Morphological features of collateral innervation and polyneuronal innervation in the skeletal muscle of presenile rats" Annals of Anatomy. 177. 251-265 (1995)

Masaru Kawabuchi：“早老大鼠骨骼肌侧支神经支配和多神经元神经支配的形态特征”解剖学年鉴。

Masaru Kawabuchi: "Expression of NOS,NCAM-H,and S100 protein in the granule cell generation pathway of the adult guinear pig olfactory bulb" Society for Neuroscience Abstracts. 22. 980- (1996)

Masaru Kawabuchi：“成年豚鼠嗅球颗粒细胞生成途径中 NOS、NCAM-H 和 S100 蛋白的表达”神经科学学会文摘。

Masaru,Kawabuchi: "Variations of collateral branching in the motor nerve fibers in the skeletal muscle fibers of adult rats" Acta Histochemica. 94. 173-182 (1992)

Masaru，Kawabuchi：“成年大鼠骨骼肌纤维中运动神经纤维侧支分支的变化”组织化学学报。

Kazuho Hirata: "Postnatal development of Schwann cells at neruomuscular junctions,with special rererence to synapse elimination" Journal of Neruocytology. 26. 799-809 (1997)

Kazuho Hirata：“神经肌肉接头处雪旺细胞的出生后发育，特别涉及突触消除”《神经细胞学杂志》。