High-content study of axonal guidance using optical protein patterning

使用光学蛋白质图案进行轴突引导的高内涵研究

• 批准号: 355603-2011
• 负责人: Costantino, Santiago
• 金额: $ 2.19万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=549779
• 关键词: content; study; axonal; guidance; using

In the embryonic environment, developing axons encounter chemical indicators that, upon processing by the growth cone, result in directed outgrowth allowing connections with specific synaptic targets. Long- and short-range guidance cues provide either attractive or inhibitory information to growth cones, leading to cytoskeletal reorganization and steering events. These cues, in the form of either diffusible factors secreted by other cells, or of proteins positioned on cell surfaces or in the extracellular matrix (ECM), take on spatial distributions that are interpreted by axons in a manner that remains to be fully deciphered.

在胚胎环境中，发育中的轴突遇到化学指示剂，这些化学指示剂在被生长锥加工后导致定向生长，从而允许与特定突触靶点连接。长距离和短距离的指导线索提供有吸引力的或抑制性的信息，生长锥，导致细胞骨架重组和转向事件。这些线索以其他细胞分泌的可扩散因子或位于细胞表面或细胞外基质（ECM）中的蛋白质的形式呈现空间分布，这些空间分布由轴突以一种仍有待完全破译的方式解释。