Age changes in cellular interactions among the endplate components (axon, Schwann cells, acetylcholine receptor, synaptic matrix) during reinnervation to the motor endplate

运动终板重新神经支配过程中终板成分(轴突、雪旺细胞、乙酰胆碱受体、突触基质)之间细胞相互作用的年龄变化

基本信息

  • 批准号:
    12670018
  • 负责人:
  • 金额:
    $ 2.05万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2002
  • 项目状态:
    已结题

项目摘要

We have already shown some age-related changes in regenerating NMJs, such as persistent damage in the terminal Schwann cell and the acetylcholine receptor (acetylcholine receptors, AChRs) region after sciatic nerve crushing injury. This research aimed at solving the relation of these changes and the signal mechanism. Cryostat sections of the soleus muscle were made, and first, after the composition element (axon, Schwann cell, AChR region) on NMJ was labeled, the expression of signal-related-substances (neuregulin (NRG), NRG receptors (erB2 and erB3) and glial cell line-derived neurotrophic factor (GDNF)) was double labeled by immunohistochemistry. Next, since NRG/erbB signaling is known to be concerned with the aggregation of AChRs, we examined the expression of dystrophin (DYS, postsynaptic cytoskeleton protein) in the AChR region as labeled by alpha-bungarotoxin. Observation was performed at the time when regeneration of NMJ was in progress (4-12 postoperative weeks).Results. In the … More young animals NRG was distributed over the full length of regenerating axons, up to a growth cone or a terminal. ErB2 and erB3 of a NRG receptor were distributed over the synaptic folds and the terminal Schwann cells. Expression of NRG/erB2 (or erB3) became transiently increased at four postoperative week. GDNF was localized in the Schwann cell strands, and synaptic folds and terminal Schwann cells in the NMJ. Expression of GDNF was reinforced as regeneration progressed, and numerous NMJs showed the strong positivity at eight postoperative week. In the aged muscles any signal-related-substance (NRG, erB2, erB3, and GDNF) showed some abnormalities (delayed expression, weak staining, malformation). 2. Derangement in the distribution of a receptor region in aged animals frequently accompanied abnormalities in the expression of DYS (weak staining and partial lack). Conclusion. 1. The interaction between the presynaptic and postsynaptic region is important for regeneration or structural specialization of the NMJ, i.e., for recovery of a physiological function. It is thought that age-related abnormalities in the distribution of signal molecules reported here relate to the insufficiency of regeneration at the NMJs. 2. The old-age-model is useful in order to study the disturbance of the signal mechanism at NMJs. Less
我们已经显示了再生NMJ中的一些年龄相关变化,例如坐骨神经挤压损伤后末端雪旺细胞和乙酰胆碱受体(乙酰胆碱受体,AChRs)区域的持续损伤。本研究旨在解决这些变化与信号机制的关系。制作比目鱼肌冰冻切片,首先标记NMJ上的组成成分(轴突、雪旺细胞、AChR区),然后免疫组化双标记神经调节蛋白(NRG)、神经调节蛋白受体(erB 2和erB 3)和胶质细胞源性神经营养因子(GDNF)的表达。接下来,由于已知NRG/erbB信号传导与AChR的聚集有关,我们检查了由α-银环蛇毒素标记的AChR区域中的抗肌萎缩蛋白(DYS,突触后细胞骨架蛋白)的表达。术后4-12周,观察NMJ再生情况。在 ...更多信息 幼年动物NRG分布在再生轴突的全长上,直至生长锥或终末。NRG受体的ErB_2和ErB_3分布于突触褶和终末雪旺细胞。术后4周,NRG/erB 2(或erB 3)表达短暂升高。GDNF定位于NMJ的许旺细胞链、突触褶和终末许旺细胞。GDNF的表达随再生的进行而增强,术后8周大量NMJ呈强阳性表达。在老年肌肉中,任何信号相关物质(NRG、erB 2、erB 3和GDNF)都显示出一些异常(延迟表达、弱染色、畸形)。2.在老年动物的受体区域的分布紊乱经常伴随着异常的DYS的表达(弱染色和部分缺乏)。结论1.突触前和突触后区域之间的相互作用对于NMJ的再生或结构特化是重要的,即,恢复生理功能据认为,与年龄相关的异常分布的信号分子在这里报告有关的再生不足的NMJ。2.老年模型是有用的,以研究在NMJ的信号机制的干扰。少

项目成果

期刊论文数量(40)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kanemaru H, Nakamura H, Isayama H, Kawabuchi M, Tashiro N: "Efferent connections of the anterior hypothalamic nucleus: a biocytin study in the cat"Brain Research Bulletin. 51(3). 219-232 (2000)
Kanemaru H、Nakamura H、Isayama H、Kawabuchi M、Tashiro N:“下丘脑前核的传出连接:猫的生物胞素研究”脑研究通报。
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    0
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J-W.He et al.: "An improved method for avulsion of lumbar nerve roots as an experimental model of nitric oxide-mediated neuronal degeneration"Brain Research Protocols. 5. 223-230 (2000)
J-W.He 等人:“一种改进的腰神经根撕脱方法,作为一氧化氮介导的神经元变性的实验模型”脑研究方案。
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    0
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Zhou C, Kawabuchi M, Songyan W, Liu W, Hirata K: "Age differences in morphological patterns of axonal sprouting and multipleinnervation of neuromuscular junctions during muscle reinnervation following nerve crush injury."Annals of Anatomy. 184. 461-472 (2
Zhou C、Kawabuchi M、Songyan W、Liu W、Hirata K:“神经挤压伤后肌肉再神经支配期间轴突萌芽和神经肌肉接头多重神经支配形态模式的年龄差异。”解剖学年鉴。
  • DOI:
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    0
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M.Kawabuchi et al.: "Morphological features of nerve terminal degeneration as part of the remodeling process in the motor endplate in adult muscles"Ultrastructural Pathology. 24. 279-289 (2000)
M.Kawabuchi 等人:“作为成人肌肉运动终板重塑过程一部分的神经末梢变性的形态特征”超微结构病理学。
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    0
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He J-W, Hirata K, Kuraoka A, Kawabuchi M: "An improved method for avulsion of lumbar nerve roots as an experimental model of nitric oxide-mediated neuronal degeneration"Brain Research Protocols. 5. 223-230 (2000)
He J-W、Hirata K、Kuraoka A、Kawabuchi M:“一种改进的腰神经根撕脱方法,作为一氧化氮介导的神经元变性的实验模型”脑研究方案。
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    0
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KAWABUCHI Masaru其他文献

KAWABUCHI Masaru的其他文献

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{{ truncateString('KAWABUCHI Masaru', 18)}}的其他基金

Interactions between Schwann cell and axon during reinnervation to the motor endplate
运动终板神经再支配过程中雪旺细胞和轴突之间的相互作用
  • 批准号:
    08670020
  • 财政年份:
    1996
  • 资助金额:
    $ 2.05万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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视神经再生过程中促进视网膜神经节细胞轴突引导的细胞和分子机制
  • 批准号:
    10433938
  • 财政年份:
    2021
  • 资助金额:
    $ 2.05万
  • 项目类别:
Cellular and molecular mechanisms promoting retinal ganglion cell axonal guidance during optic nerve regeneration
视神经再生过程中促进视网膜神经节细胞轴突引导的细胞和分子机制
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The role of axonal guidance genes in the regulation of dopamine-mediated behaviors and synaptic connectivity
轴突引导基因在多巴胺介导的行为和突触连接调节中的作用
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    10381947
  • 财政年份:
    2021
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    $ 2.05万
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Elucidation of the signal integration mechanism on R-Ras family small GTPase in axonal guidance
阐明 R-Ras 家族小 GTPase 轴突引导中的信号整合机制
  • 批准号:
    17K07340
  • 财政年份:
    2017
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    $ 2.05万
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Mechanisms for Axonal Guidance Using Living Tissue Engineered Scaffolds
使用活组织工程支架进行轴突引导的机制
  • 批准号:
    9335678
  • 财政年份:
    2015
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Mechanisms for Axonal Guidance Using Living Tissue Engineered Scaffolds
使用活组织工程支架进行轴突引导的机制
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    8983595
  • 财政年份:
    2015
  • 资助金额:
    $ 2.05万
  • 项目类别:
High-content study of axonal guidance using optical protein patterning
使用光学蛋白质图案进行轴突引导的高内涵研究
  • 批准号:
    355603-2011
  • 财政年份:
    2015
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    $ 2.05万
  • 项目类别:
    Discovery Grants Program - Individual
High-content study of axonal guidance using optical protein patterning
使用光学蛋白质图案进行轴突引导的高内涵研究
  • 批准号:
    355603-2011
  • 财政年份:
    2014
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    $ 2.05万
  • 项目类别:
    Discovery Grants Program - Individual
Biologic Roles of Novel Axonal Guidance Genes in Isolated GnRH Deficiency
新型轴突引导基因在孤立性 GnRH 缺乏症中的生物学作用
  • 批准号:
    8700856
  • 财政年份:
    2014
  • 资助金额:
    $ 2.05万
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High-content study of axonal guidance using optical protein patterning
使用光学蛋白质图案进行轴突引导的高内涵研究
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