Identification of troponin subunits which regulate the length dependent change in the Ca sensitivity in cardiac muscles
调节心肌 Ca 敏感性长度依赖性变化的肌钙蛋白亚基的鉴定
基本信息
- 批准号:08670063
- 负责人:
- 金额:$ 1.54万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Cardiac muscle contraction is significantly altered by a change in initial muscle length before contraction. This is considered to be due to the crossbridge-dependent change in the affinity of troponin-C (Tn-C) for Ca ion. The hypothesis was examined to measure the relation between Ca ion concentration and tension (pCa-tension relation) at different muscle lengths in intact and skinned preparations of mammalian cardiac muscles. In intact preparations, the papillary muscles treated with ryanodine were repetitively stimulated and tetanus was induced for measuring pCa-tension relation at different muscle lengths in the presence or absence of isoproterenol (ISO). ISO increased the length-dependent shift of pCa-tension relation. Thin trabeculae were treated with Triton X and stored in 50% glycerol containing relaxing solution. pCa-tension relations at different muscle lengths were measured before and after phosphorylation by A-kinase. After phosphorylation, length-dependent shift of pCa-tension relation was lessened. These results suggest that Tn-I and/or C-protein which can be phosphorylated by A-kinase might be involved in the signal transduction from crossbridges to Tn-C.The different effects of A-kinase dependent phosphorylation in intact and skinned preparation should be further investigated.
收缩前初始肌肉长度的变化会显著改变心肌收缩。这被认为是由于肌钙蛋白-C(Tn-C)对Ca离子的亲和力的跨桥依赖性变化。该假设进行了检查,以测量钙离子浓度和张力(pCa-张力关系)之间的关系,在不同的肌肉长度在完整的和皮肤的准备哺乳动物心肌。在完整的准备,用兰尼定处理的乳头肌重复刺激和破伤风诱导测量pCa-张力关系在不同的肌肉长度在存在或不存在异丙肾上腺素(ISO)。ISO增加了pCa-张力关系的长度依赖性位移。用Triton X处理薄骨小梁,并储存在含有50%甘油的松弛溶液中。在不同的肌肉长度的pCa-张力的关系进行了测量之前和之后的磷酸化的A-激酶。磷酸化后,pCa-张力关系的长度依赖性移位减少。这些结果表明,可被A-激酶磷酸化的Tn-I和/或C-蛋白可能参与了从横桥到Tn-C的信号转导。A-激酶依赖的磷酸化在完整和去皮制剂中的不同作用有待进一步研究。
项目成果
期刊论文数量(23)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kurihara, S.: "Regulation of cardiac muscle contraction by intracellular Ca^<2+> concentration change" Tokyo Jikei Medical Journal. 111. 265-278 (1996)
Kurihara, S.:“细胞内Ca^2浓度变化对心肌收缩的调节”东京医疗医学杂志。
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- 影响因子:0
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栗原 敏: "ストレプトゾトシン誘発性糖尿病ラット心筋における細胞内Ca動態と収縮特性"
Satoshi Kurihara:“链脲佐菌素诱导的糖尿病大鼠心肌细胞内 Ca 动力学和收缩特性”
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- 影响因子:0
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栗原敏: "細胞内Ca^<2+>濃度変化による心筋の収縮制御" 東京慈恵会医科大学雑誌. 111・3. 265-278 (1996)
Satoshi Kurihara:“通过改变细胞内Ca^<2+>浓度来控制心肌收缩”,东京慈惠大学医学部杂志111・3(1996)。
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- 影响因子:0
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Kurihara,S.: "Physiological significance of the change in the Ca^<2+> sensitivity of the contractile elements in cardiac muscle" Molecular and Cellular Mechanisms of Cardiovascular Regulation. 281-290 (1996)
Kurihara,S.:“心肌收缩元件的 Ca^2 敏感性变化的生理意义”心血管调节的分子和细胞机制。
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- 影响因子:0
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- 通讯作者:
Kurihara, S.: "Length dependence of Ca^<2+>-tension relationship in aequorin-injected ferret papillary muscles" American Journal of Physiology. 273. H1068-H1074 (1997)
Kurihara,S.:“注射水母蛋白的雪貂乳头肌中Ca^2-张力关系的长度依赖性”美国生理学杂志。
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KURIHARA Satoshi其他文献
KURIHARA Satoshi的其他文献
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{{ truncateString('KURIHARA Satoshi', 18)}}的其他基金
Proposal of adaptive coordination formation control mechanism for multiagent planning
多智能体规划自适应协调编队控制机制的提出
- 批准号:
23300058 - 财政年份:2011
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Proposal of hierarchy structure emergence mechanism for large scale complex systems
大规模复杂系统层次结构涌现机制的提出
- 批准号:
23650071 - 财政年份:2011
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Molecular mechanisms of an improvement in prognosis in the mouse model of dilated cardiomyopathy treated by inhibition of the RAA system
抑制 RAA 系统治疗扩张型心肌病小鼠模型预后改善的分子机制
- 批准号:
22300130 - 财政年份:2010
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$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Proposal of top-down controllable multiagent coordination mechanism
自上而下的可控多主体协调机制的提出
- 批准号:
20500133 - 财政年份:2008
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular mechanism of contractile dysfunction and the alteration of intracellular Ca^<2+> handling caused by mutation of cardiac troponin T
心肌肌钙蛋白T突变引起收缩功能障碍及细胞内Ca^2处理改变的分子机制
- 批准号:
19500357 - 财政年份:2007
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular mechanisms of Ca^<2+> uptake by sarcolipin in cardiac sarcoplasmic reticulum.
心脏肌浆网肌磷脂摄取Ca ^ 2 的分子机制。
- 批准号:
16500269 - 财政年份:2004
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Research on assessment system of medical dental students' clinical competence before entering clinical clerkship
医学牙科学生进入临床实习前临床能力评价体系研究
- 批准号:
13800002 - 财政年份:2001
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Special Purposes
A Basis Study of Ethnographies in Southwest of China -Mainly Yunnan Area-
西南地区——主要是云南地区——民族志基础研究
- 批准号:
13610368 - 财政年份:2001
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Production of new recombinant aequorin and its physiological application
新型重组水母发光蛋白的制备及其生理应用
- 批准号:
13557004 - 财政年份:2001
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Intracellular Ca^<2+> regulation in the myocardium with ryanodine receptor type 2(+/-) heterozygous mouse
兰尼定受体2型(/-)杂合小鼠心肌细胞内Ca^<2>调节
- 批准号:
13670048 - 财政年份:2001
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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