Endocrine cell tumors of gastrointestinal tract : molecular biological study of their histogenesis progression, and metastasis

胃肠道内分泌细胞肿瘤：其组织发生进展和转移的分子生物学研究

• 批准号: 08670195
• 负责人: IWAFUCHI Mitsuya
• 金额: $ 1.41万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670195/
• 关键词: Carcinoid; Endocrine cell carcinoma; Small cell carcinoma; Paraneuroma; Gut hormone; Cell culture

During the period of past two years of this project, about 400 gastrointestinal endocrine cell tumors (GECT) of Japanese and six cell lines of endocrine cell carcinoma were analyzed.1. CLASSIFICATION : GECT were divided into two main groups : carcinoid tumor (CD), low-grade malignancy and endocrine cell carcinoma (ECC), high-grade malignacy.Histological differential diagnosis and the new classification according to ocurring sites of GECT were established.2. HISTOGENESIS : CD was suspected to be mainly derived from immature endocrine cell. ECC was hypothesized to derive from neoplastic endocrine cell in mucosal tubular adenocarcinoma/adenoma, totipotential stem cell, CD,or immature endocrine cell. The first pathway was suspected to be most frequent, followed by the second. Difference in p53 protein overexpression between CD and ECC confirmed the difference in their histogenetic pathway.3. PROGRESSION AND METASTASIS : Cell proliferation activity of GECT was confirmed to correlate well with tumor progression and metastasis. For clinical hormone analysis to assess tumor recurrence and metastasis, GECT should be subdivided into three groups ; argentaffin (serotonin-producing) CD,argyrophi1 (peptide-producing) CD,and ECC (frequently serotonin-producing).4. CELL LINES : Four cell lines established from human esophageal, gastric and rectal ECC well preserved hormone (serotonin, peptide YY,calcitonin)-production. One esophageal cell line was effected by Vincristin and Mitomycin.

本项目历时两年，对约400例日本胃肠道内分泌细胞瘤（GECT）和6种内分泌细胞癌细胞系进行了分析。 1．分类：GECT主要分为两大类：类癌（CD）、低度恶性肿瘤和内分泌细胞癌（ECC）、高度恶性肿瘤。建立了组织学鉴别诊断和根据GECT发生部位的新分类。 2．组织发生：怀疑CD主要来源于未成熟的内分泌细胞。 ECC被假设源自粘膜管状腺癌/腺瘤中的肿瘤性内分泌细胞、全能干细胞、CD或未成熟内分泌细胞。第一条途径被认为是最常见的，其次是第二条。 CD和ECC之间p53蛋白过度表达的差异证实了它们的组织发生途径的差异。 3．进展和转移：GECT 的细胞增殖活性被证实与肿瘤进展和转移密切相关。对于临床激素分析以评估肿瘤复发和转移，GECT 应分为三组； argentaffin（产生血清素）CD、argyrophi1（产生肽）CD 和 ECC（经常产生血清素）。4．细胞系：从人食管、胃和直肠 ECC 中建立的四种细胞系，保存完好的激素（血清素、肽 YY、降钙素）生产。一种食管细胞系受到长春新碱和丝裂霉素的影响。

项目成果

Nishikura K: "Progress in Gastric Cancer Research 1997" Mondvzzi Editore, 400 (1997)

Nishikura K：“1997 年胃癌研究进展”Mondvzzi Editore，400 (1997)

渡辺 英伸: "食道 dysplasia(異形成)の組織診断" 胃と腸. 31. 705-709 (1996)

Hienobu Watanabe：“食管发育不良的组织学诊断”《胃与肠》31. 705-709 (1996)。

Yagi K: "A minute rectal carcinoma with massive submucasal invasion and lymph-mode metastasis" Endosiopy. 28. 161- (1996)

Yagi K：“一种微小的直肠癌，具有大量粘膜下浸润和淋巴转移”内窥镜检查。

Matsuda K: "Ulcerative colotic with overexpression of p53 preceding overt histological abnormalities of the opithelium" J Gastroenteol. 31. 860-867 (1996)

Matsuda K：“溃疡性结肠炎伴有 p53 过度表达，先于上皮细胞的明显组织学异常”J Gastroenteol。

何汝朝: "多彩な内視鏡像を呈した早期胃腺扁平上皮癌の1例" 胃と腸. 31. 913-918 (1996)

何超：“早期胃腺鳞癌伴多种内镜表现一例”《胃与肠》31. 913-918(1996)。