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Endocrine cell tumors of gastrointestinal tract : histogenesis, growth, metastasis and racial differences

胃肠道内分泌细胞肿瘤：组织发生、生长、转移和种族差异

基本信息

批准号：
10670155
负责人：
IWAFUCHI Mitsuya
金额：
$ 2.05万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670155/
关键词：
Carcinoid Endocrine cell carcinoma Small call carcinoma Paraneuroma Gut hormone Cell culture ペプチド消化管ホルモン p53

项目摘要

During the period of past two years of this particular project, endocrine cell tumors of the gastrointestinal tract were collected and analyzed.1. Endocrine cell tumors of the gastrointestinal tract were divided into two groups : carcinoid tumor(CD), low-grade malignancy and endocrine cell carcinoma(ECC), high-grade malignancy. Histological differential diagnosis between these two entities was established.2. CD was thought to be mainly derived from immature endocrine cells. ECC was hypothesized to derive from neoplastic endocrine cell in mucosal tubular adenocarcinoma orade noma, totipotential stem cell, CD, or immature endocrine cell. The first pathway was suspected to be most frequent, followed by the second. These hypothesis was confirmed by molecular level on p53 and LOH.3. New classification on CD and ECC according to different ocurring sites was established.4. Difference of p58 protein overexpression and cell proliferation activity between CD and ECC were established. p53 protein overexpression and cell proliferation activity were decreased or lost even in well differentiated ECC cells.5. Four cell lines established from human esophageal, gastric and rectal ECC revealed a well preservation of hormone(srotonin, peptide YY, calcitonin)-production. Cell line from esophageal ECC was effected by Vincristin and Mitomycin.

在过去的两年里，这个特殊的项目，胃肠道内分泌细胞肿瘤的收集和分析。胃肠道内分泌细胞肿瘤分为低度恶性的类癌（CD）和高度恶性的内分泌细胞癌（ECC）。建立了这两种实体之间的组织学鉴别诊断。CD被认为主要来源于未成熟的内分泌细胞。ECC可能来源于黏膜管状腺癌或坏疽性口炎的肿瘤性内分泌细胞、全能干细胞、CD或未成熟的内分泌细胞。第一种途径被怀疑是最常见的，其次是第二种。这些假说在p53和LOH的分子水平得到证实。根据不同发生部位建立了CD和ECC的新分类。4. CD和ECC的p58蛋白表达和细胞增殖活性存在差异。即使在分化良好的ECC细胞中，p53蛋白过表达和细胞增殖活性也降低或丧失.从人食管、胃和直肠ECC建立的四种细胞系显示激素（srotonin、肽YY、降钙素）生产的良好保存。食管癌细胞株能被丝裂霉素和曲马多菌素所作用。