Establishment of hypertansive congenic Strains

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We have been developing congenic rat strains that possess the hypertension genes of stroke-prone spontaneously hypertensive rats (SHRSP) on the background of a normotensive control, Wistar-Kyoto rats (WKY).1) Preparation of genetic markersa) We selected several genes probably located in the hypertension-related region on rat chromosome 1by comparing rat genome with mouse and human genomes. We isolated 11 genetic markers from the genomic clones of the corresponding rat genes.b) We applied the randomly amplified microsatellite polymorphisms (RAMP) method (Wu et al., 1994) to get more than 100 codominant markers that are polymorphic between SHRSP and WKY.2) Construction of the congenic strainsSHRSP were sequencially backcrossed to WKY for 5 generations to get the semi-congenic strains (more than 95% of genome were from WKY). Genotypes of the backcross rats of each generation were screened by genotyping of microsatellite markers. We have so far obtained 6 semi-congenic strains that have different regions on rat chomosome 1.We now check blood pressure of these strains as well as backcross them further to make complete congenic strains.