Identification of QTLs for various lipid phenotypes In the stroke-prone spontaneously hypertensive rats and their relation to the susceptibility to cerebral stroke

脑卒中易发性高血压大鼠不同脂质表型QTL的鉴定及其与脑卒中易感性的关系

• 批准号: 11670681
• 负责人: NABIKA Toru
• 金额: $ 0.9万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670681/
• 关键词: stroke-prone spontaneously hypertensive rats; genetics; quantitative trait locus; cholesterol; cerebral stroke; triglyceride; 脳卒中易発症自然発症高血圧ラット; 連鎖解析; 量的形質; QTL; コレステロール; 脳卒中

The stroke-prone spontaneously hypertensive rats (SHRSP) are known to have very high incidence of cerebral stroke. In addition, this strain has been known to have an abnormality in the lipid metabolism when compared with the normotensive control, Wistar-Kyoto rats (WKY). As epidemiological studies in humans indicated that the lipid metabolism had a close relation to the susceptibility to cerebral stroke, the abnormal lipid metabolism might be one of the essential factors responsible for the stroke susceptibility in SHRSP. In this study, we therefore performed genetic analyses on the traits related to the lipid metabolism in F2 rats obtained between SHRSP and WKY. We found (1) quantitative trait loci (QTLs) for the serum cholesterol level on chromosomes 3, 5, 7, 15, and 16, (2) a QTL for the serum triglyceride level on chromosome 8, and 16, (3) QTLs different between male and female rats observed in several chromosomal regions. On the chromosome 5, a previous study suggested a gene responsible for the susceptibility to the cerebral stroke. It is of interest whether this susceptibility locus and the QTL for the serum cholesterol are in the same region or not. We are now constructing congenic strains for these QTLs to confirm the effects of these chromosomal regions either on the lipid metabolism or on cerebral stroke.

易卒中的自发性高血压大鼠（SHRSP）是脑卒中的高发动物。此外，已知该品系与血压正常的对照Wistar-Kyoto大鼠（WKY）相比，脂质代谢异常。流行病学研究表明，脂质代谢与脑卒中易感性密切相关，脂质代谢异常可能是SHRSP脑卒中易感性的重要因素之一。因此，在本研究中，我们进行了遗传分析的性状有关的脂代谢的F2大鼠之间的SHRSP和WKY。在第3、5、7、15、16号染色体上发现了控制血清胆固醇水平的数量性状位点（QTL）;在第8、16号染色体上发现了控制血清甘油三酯水平的QTL;在5号染色体上，先前的研究表明有一个基因负责脑卒中的易感性。该易感基因座与血清胆固醇的QTL是否位于同一区域值得关注。我们现在正在构建这些QTL的同源株系，以确认这些染色体区域对脂质代谢或脑卒中的影响。

项目成果

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