Development of evaluation method on the health effects of multicomponent organic vapors using biological exposure indices

利用生物暴露指数评价多组分有机蒸气的健康影响方法的开发

• 批准号: 08670464
• 负责人: HORI Hajime
• 金额: $ 1.41万
• 依托单位: University of Occupational and Environmnental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670464/
• 关键词: methanol; toluene; methyl ethylketone; inhalation; formic acid; hippuric acid; metabolites in urine; organic solvent

Most organic sovents used in work environments are mixture of several solvents. This study addresses the influence of simultaneous exposure of organic vapors by inhalation on their metabolic disposition.Wister male rats (8 weeks old) were exposed to methanol, toluene, MEK and their mixtures. The exposure periods were 6 hours a day for 1day and 1 month. After the termination of the exposure, the rats were transferred into separate metabolic chambers and urine were collected individually at 18 and 42 hours after the exposure. Blood samples were also collected from tale of the rats temporary up to 3 hours after the exposure. Major metabolites of the solvents in urine and the solvent concentrations in blood were determined.For methanol-toluene system, compared with single exposure, simultaneous exposure resulted in larger amount of excreted hippuric acid and formic acid in urine during the exposure period. However, hippuric acid excretion rate in the simultaneous exposure group was significantly smaller than that in the toluene exposure group during 0-18 hours, and there was no significant difference observed during 18-42 hours. Biological half time (t<@D21/2@>D2) of toluene in blood in the simultaneous exposure group was 1.2 (]SY.+-。[) 0.1 hr hours, which was significantly shorter than that in the toluene exposure group (2.0 (]SY.+-。[) 0.4 hr). On the other hand, the half time of methanol in blood was not dignificantly different between both methanol (2.7 (]SY.+-。[) 0.4hr) and simultaneous exposure (2.6 (]SY.+-。[) 0.6hr) groups. The results suggest that methanol promotes toluene metabolic disposition, but toluene may not affect significant influence in the methanol metabolites. For MEK-toluene system, the biological half time of toluene in blood for simultaneous exposure group was longer than that for toluene exposure group, suggesting that the the metabolic disposition of toluene was inhibited by MEK.

在工作环境中使用的大多数有机溶剂是几种溶剂的混合物。本研究以雄性Wister大鼠（8周龄）为研究对象，研究了甲醇、甲苯、甲乙酮及其混合物同时吸入有机蒸气对其代谢的影响。暴露时间为每天6小时，持续1天和1个月。暴露结束后，将大鼠转移至单独的代谢室中，并在暴露后18和42小时分别收集尿液。还在暴露后3小时内暂时从大鼠尾部收集血液样本。结果表明，与单次暴露相比，甲醇-甲苯体系中，同时暴露导致马尿酸和甲酸在尿液中的排泄量增加。但同时接触组马尿酸排泄率在0-18 h显著小于甲苯接触组，在18-42 h无显著差异。同时接触组血中甲苯的生物半衰期（t<@ D_（21/2）@> D_2）为1.2（± SY）。[] 0.1小时，显著短于甲苯暴露组（2.0（]SY. ±. [）0.4小时）。另一方面，甲醇在血液中的半衰期在两种甲醇之间没有显著差异（2.7（±）SY.）。[] 0.4hr）和同时暴露（2.6（]SY. ±. 0.6小时组。结果表明，甲醇促进甲苯的代谢处置，但甲苯可能不会影响甲醇代谢产物的显着影响。在MEK-甲苯体系中，甲苯在血液中的生物半衰期，同时暴露组明显长于甲苯暴露组，说明甲苯的代谢处置受到MEK的抑制。

项目成果

Hori, H et al.: "Effect of Simultaneous Exposure to Methanol and Toluene Vapor on Their Metabolites in Rats" Abstracts of 3rd IOHA International Scientific Conference. (1997)

Hori, H 等人：“同时接触甲醇和甲苯蒸气对其代谢物的影响”，第三届 IOHA 国际科学会议摘要。

保利 一, 他: "メタノール,トルエン混合蒸気吸入曝露ラットにおける尿中代謝物および血中有機溶剤濃度の測定" 日本衛生学雑誌. 52(1). 383 (1997)

Hajime Mori 等人：“吸入甲醇和甲苯混合蒸气的大鼠的尿液代谢物和血液有机溶剂浓度的测量”，日本卫生杂志 52(1)。

保利 一, 他: "メタモ-ル,トルエン混合蒸気吸入曝露ラットにおける尿中代謝物および血中有機溶剤濃度の測定" 日本衛生学雑誌. 52(1). 383 (1997)

Hajime Mori 等人：“吸入异丙醇和甲苯混合蒸气的大鼠的尿代谢物和血液有机溶剂浓度的测量”，日本卫生杂志 52(1) (1997)。

保利一: "メタノール,トルエン混合蒸気吸入曝露ラットにおける尿中代謝物および血中有機溶剤濃度の測定" 日本衛生学雑誌. 52(1). (1997)

Kazu Mori：“吸入甲醇和甲苯混合蒸气的大鼠的尿液代谢物和血液有机溶剂浓度的测量”日本卫生杂志 52(1) (1997)。

Hori, Hajime et al.: "Measurement of organic solvents in blood and metabolites in urine after exposed to methanol and toluene vapor mixture in rats." Japanese Journal of Hygiene. 52(1). 383 (1997)

Hori、Hajime 等人：“测量大鼠暴露于甲醇和甲苯蒸气混合物后血液中的有机溶剂和尿液中的代谢物。”