Circulating autoantibody to type II alveolar pneumocytes in patients with idiopathic interstial pneumonia

特发性间质性肺炎患者 II 型肺泡肺细胞循环自身抗体

• 批准号: 08670642
• 负责人: HOMMA Yukihiko
• 金额: $ 1.34万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670642/
• 关键词: idiopathic interstitial pneumonia; collagen-vascular disease; autoimmune; 自己免疫

The existence of circulating autoantibody to lung proteins in patients with cryptogenic fibrosing alveolitis was reported. To evaluate the role of this autoantibody, we attemted to detect this in 29 patients with idiopathic interstitial pneumonia (IIP), and compared the clinical characteristics between the patients with or without this autoantibody. Serum IqG was purified from plasma of the patients by affinity chromatoqraphy. Cytospin preparations of human A549 type II alveolar cells were fixed by acetone and blocked with protein A and normal human serum. They were incubated with purified patient's IgG.After washing, they were incubated with protein A-alkalinephosphatase conjugate, and visualized by Vector Red. plasma from 10 of 29 patients (34g) gave positive staining. Serm IgG and IgE levels were significantly higher in patients with autoantibody than those without it. Number of eosinophils in bronchoalveolar lavage fiuids were significantly lower in patients with autoantibody than those without it. There were no significant differences in age, sex, pulmonary functions and other laboratory data. Immunohistochemical staining of lung tissues revealed the localization of the autoantibody in cytoplasm of type II alveolar pneumocyte by using confocal laser scanning microscope. Dott blotting with purified serum IgG from patients with positive antibody group also showed positive staning in cytosol fraction of A549 cells. These results suggest that circulating autoantibody to type II alveolar cells could be detected in about 1/3 of the patients with IIP,which localized to cytosol of type II alveolar pneumocyte, and these patients may have different mode of pulmonary inflammation.

报告了隐源性纤维性肺泡炎患者存在循环抗肺蛋白自身抗体的情况。为了评估这种自身抗体的作用，我们试图检测29例特发性间质性肺炎(IIP)患者的这种抗体，并比较有或没有这种自身抗体的患者的临床特征。用亲和层析法从患者血浆中提纯血清IqG。用丙酮固定A549人肺泡II型细胞的胞浆制剂，并用蛋白A和正常人血清阻断。与纯化的患者免疫球蛋白孵育，洗涤后与蛋白A-碱性磷酸酶结合物孵育，载体红显色。29例患者中有10例(34g)血浆呈阳性染色。自身抗体阳性患者血清Ig G、Ig E水平显著高于无自身抗体患者。自身抗体阳性患者支气管肺泡灌洗液中嗜酸性粒细胞数量明显低于无自身抗体阳性患者。在年龄、性别、肺功能和其他实验室数据方面没有显著差异。共聚焦激光扫描显微镜下肺组织免疫组织化学染色显示自身抗体定位于肺泡II型细胞胞浆内。抗体阳性组患者纯化的血清免疫球蛋白斑点杂交也显示A549细胞胞浆部分呈阳性。提示IIP患者中约1/3可检测到抗肺泡型细胞自身抗体，抗体定位于肺泡型细胞胞浆，这些患者可能存在不同的肺部炎症模式。

项目成果

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