REPIGMENTAION OF VITILIGO AND MELANOCYTE MIGRATION -REGULATING MECHANISMS OF MELANOCYTE MIGRATION IN EPIDERMIAL-FOLLICULAR UNIT-

我们已经表明，人毛囊外根鞘（ORS-MC）中的非活性黑素细胞可通过抗前黑素体抗体而不是酪氨酸酶、TRP-1和TRP-2的抗体来识别（J Invest Dermatol，106：28- 35，1996）。这些黑素细胞可以培养（J dermSci，12：204，1996）。我们开发了一种新的技术，通过改变钙的浓度来培养人黑素细胞（色素细胞研究，9：58- 62，1996）。利用器官培养技术培养人的毛发，发现ORS-MCs在体外可被激活并呈DOPA阳性。在ORS-MCs活化的毛发中，RT-PCR显示酪氨酸酶转录本在活化后出现（PigmentCellRes，10：429，1997）。接下来，我们发现ET-1通过异源三聚体G蛋白和磷脂酶A2（PLA 2）诱导黑素细胞迁移，bFGF和LTC 4通过酪氨酸激酶和PLA 2诱导黑素细胞迁移（J Invest Dermatol，108：573，1997）。ras转染的黑素细胞研究表明，激活的ras参与黑素细胞迁移的诱导（J Derm Sci，15：128，1997）。活化的ras转染的黑色素瘤细胞比亲本细胞具有更高的迁移能力，并且PLA 2在ras转染的细胞中被活化（J Invest Dermatol，109：407，1997）。发现E-钙粘蛋白的表达被UV照射降低（J Invest Dermatol 106：1320- 1324，1996）。我们还发现黑素瘤细胞缺乏α或β PKC亚种，β型PKC在佛波酯刺激细胞生长中起重要作用（J Cell Physiol 167：406- 412，1996）。我们认为ORS-MCs可以被适当的刺激物激活，增殖，迁移到周围表皮。我们的研究结果将为制定治疗色素脱失的新策略提供有用的信息。

We have shown that the inactive melanocytes in the outer root sheath of the human hair follicles (ORS-MCs) are identifiable by anti-premelanosomal antibodies, but not antibodies to tyrosinase, TRP-1 and TRP-2 (J Invest Dermatol, 106 : 28-35,1996). These melanocytes could be cultivated (J derm Sci, 12 : 204,1996). We developed a new technique to cultivate human melanocytes using switching the conCentration of calcium (Pigment Cell Research, 9 : 58-62,1996). Using organ culture technique to cultivate the hairs, we found that ORS-MCs can become activated and DOPA-positive in vitro. In the hairs with activated ORS-MCs, RT-PCR revealed that tyrosinase transcript appeared after activation (Pigment Cell Res, 10 : 429,1997). Next, we found that melanocyte migration is induced by ET-1 via heterotrimeric G protein and phospholipase A2 (PLA2), and by bFGF and LTC4 via tyrosine kinase and PLA2 (J Invest Dermatol, 108 : 573,1997). Ras-transfected melanocyte study revealed that activated ras is involved in the induction of melanocyte migration (J Derm Sci, 15 : 128,1997). Activated ras-transfected melanoma cells have higher potency of migaration than parent cells, and that PLA2 is activated in ras transfected cells (J Invest Dermatol, 109 : 407,1997). The expression of E-cadherin was found to be reduced by UV irradiation (J Invest Dermatol 106 : 1320-1324,1996). We also found that melanoma cells lack either alpha or beta PKC subspecies and beta type PKC plays an important role in pholbor ester stimulation of cell growth (J Cell Physiol 167 : 406-412,1996). We believe that ORS-MCs can be activated by proper stimulants, proliferate, migrate into surrounding epidermis. Our findings will provide a useful information in establishing a new strategy of the treatment for depigmented disorders.