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REPIGMENTAION OF VITILIGO AND MELANOCYTE MIGRATION -REGULATING MECHANISMS OF MELANOCYTE MIGRATION IN EPIDERMIAL-FOLLICULAR UNIT-

白癜风的色素沉着和黑素细胞迁移-表皮-毛囊单位黑素细胞迁移的调节机制-

基本信息

批准号：
08670968
负责人：
HORIKAWA Tatsuya
金额：
$ 1.02万
依托单位：
KOBE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670968/
关键词：
melanocyte vitiligo hair follicle migration ras tyrosinase PLA2 pigment melanocyte outer root sheath 毛包 piebaldism 色素拡大紫外線 TPA

项目摘要

We have shown that the inactive melanocytes in the outer root sheath of the human hair follicles (ORS-MCs) are identifiable by anti-premelanosomal antibodies, but not antibodies to tyrosinase, TRP-1 and TRP-2 (J Invest Dermatol, 106 : 28-35,1996). These melanocytes could be cultivated (J derm Sci, 12 : 204,1996). We developed a new technique to cultivate human melanocytes using switching the conCentration of calcium (Pigment Cell Research, 9 : 58-62,1996). Using organ culture technique to cultivate the hairs, we found that ORS-MCs can become activated and DOPA-positive in vitro. In the hairs with activated ORS-MCs, RT-PCR revealed that tyrosinase transcript appeared after activation (Pigment Cell Res, 10 : 429,1997). Next, we found that melanocyte migration is induced by ET-1 via heterotrimeric G protein and phospholipase A2 (PLA2), and by bFGF and LTC4 via tyrosine kinase and PLA2 (J Invest Dermatol, 108 : 573,1997). Ras-transfected melanocyte study revealed that activated ras is involved in the induction of melanocyte migration (J Derm Sci, 15 : 128,1997). Activated ras-transfected melanoma cells have higher potency of migaration than parent cells, and that PLA2 is activated in ras transfected cells (J Invest Dermatol, 109 : 407,1997). The expression of E-cadherin was found to be reduced by UV irradiation (J Invest Dermatol 106 : 1320-1324,1996). We also found that melanoma cells lack either alpha or beta PKC subspecies and beta type PKC plays an important role in pholbor ester stimulation of cell growth (J Cell Physiol 167 : 406-412,1996). We believe that ORS-MCs can be activated by proper stimulants, proliferate, migrate into surrounding epidermis. Our findings will provide a useful information in establishing a new strategy of the treatment for depigmented disorders.

我们已经证明，人毛囊外根鞘（ORS-MCs）的失活黑色素细胞可被抗黑素体前抗体识别，但不能被酪氨酸酶、TRP-1和TRP-2抗体识别（J .中国皮肤医学杂志，16:28-35,1996）。这些黑素细胞可以被培养（皮肤科学，12:204,1996）。我们开发了一种利用改变钙浓度培养人类黑素细胞的新技术（色素细胞研究，9:58-62,1996）。利用器官培养技术培养毛发，我们发现ORS-MCs可以在体外激活并呈多巴胺阳性。在ORS-MCs激活的毛发中，RT-PCR显示激活后出现酪氨酸酶转录物（色素细胞研究，10:429,1997）。接下来，我们发现ET-1通过异三聚体G蛋白和磷脂酶A2 （PLA2）诱导黑素细胞迁移，bFGF和LTC4通过酪氨酸激酶和PLA2诱导黑素细胞迁移（J Invest Dermatol, 108: 573,1997）。ras转染黑素细胞的研究表明，激活的ras参与了黑素细胞迁移的诱导（J Derm Sci, 15: 128,1997）。激活ras转染的黑色素瘤细胞比亲本细胞具有更高的迁移能力，并且PLA2在ras转染的细胞中被激活（J .中华皮肤医学杂志，19:407,1997）。E-cadherin在紫外光照射下的表达降低[J] .中国生物医学工程学报（英文版），1996(3):334 - 334。我们还发现黑色素瘤细胞缺乏α或β PKC亚种，β型PKC在酚酯刺激细胞生长中起重要作用（J cell physical, 167: 406-412,1996）。我们认为ORS-MCs可以通过适当的刺激物激活，增殖，迁移到周围表皮。我们的发现将提供一个有用的信息，建立一个新的策略治疗色素脱失症。