Investigation of the usefulness of pentoxifylline for liver preservation

我们研究了持续低温灌注保存（CHPP）和己酮茶碱（PTX）联合应用于非心脏供者肝移植（NHBD）的有效性。本研究由三个实验组成。(1)我们进行了NHBD猪肝移植。在本实验中，我们采购后使用CHPP，将PTX放入保存液中。因此，我们得出结论，CHPP联合PTX是改善NHBD肝移植功能的有效方法。(2)本研究的目的是确定PTX预处理是否能影响大鼠肝脏热缺血损伤后一氧化氮（NO）的释放并对其有益。因此，PTX预处理增加了热缺血时NO的产生。PTX对大鼠肝脏热缺血损伤有预防作用，NO对大鼠肝脏热缺血损伤有抑制作用。(3)本研究采用CHPP法保存大鼠肝脏，并探讨哪种溶液最适合作为灌注液。因此，uw -葡萄糖酸盐溶液被认为适合用于CHPP，因为它可以抑制蛋白质水解并保护肝细胞。

We have investigaed that the usefulness of a combination of continuous hypothermic perfusion preservation (CHPP) and pentoxifylline (PTX) for liver transplantation from non-heart beating donors (NHBD). Three experiments constituted this study. (1) We performed porcine liver transplantation from NHBD. In this experiment, we used CHPP after procurement and put PTX in preservation solution. As a result, we concluded that CHPP with PTX is a useful procedure to improve the function of liver grafts from NHBD. (2) The purpose of this study is to determine whether PTX pretreatment could influence nitric oxide (NO) release and have a beneficial effect on warm ischemic injury in rat liver. As a result, PTX pretreatment increase NO production during warm ischemia. And PTX prevent warm ischemic injury and NO inhibit warm ischemic injury in rat liver. (3) In this study, we preserved rat livers by CHPP, and investigated what solution was most suitable as the perfusate. As a result, the UW-gluconate solution was considered suitable for CHPP, because it suppressed proteolysis and protected liver cells.