Investigation of the usefulness of pentoxifylline for liver preservation

己酮可可碱在肝脏保存中的有效性研究

• 批准号: 08671387
• 负责人: KOZAKI Koichi
• 金额: $ 1.47万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671387/
• 关键词: Pentoxifylline; nitric oxide; continuous hypothermic perfusion preservation (CHPP); Pentoxifylline; UW-gluconate液; University of Wisconsin solution; 肝のviability; 心停止ドナー; 低温持続灌流保存法; 死体肝移植; 再灌流障害; 電極法

We have investigaed that the usefulness of a combination of continuous hypothermic perfusion preservation (CHPP) and pentoxifylline (PTX) for liver transplantation from non-heart beating donors (NHBD). Three experiments constituted this study. (1) We performed porcine liver transplantation from NHBD. In this experiment, we used CHPP after procurement and put PTX in preservation solution. As a result, we concluded that CHPP with PTX is a useful procedure to improve the function of liver grafts from NHBD. (2) The purpose of this study is to determine whether PTX pretreatment could influence nitric oxide (NO) release and have a beneficial effect on warm ischemic injury in rat liver. As a result, PTX pretreatment increase NO production during warm ischemia. And PTX prevent warm ischemic injury and NO inhibit warm ischemic injury in rat liver. (3) In this study, we preserved rat livers by CHPP, and investigated what solution was most suitable as the perfusate. As a result, the UW-gluconate solution was considered suitable for CHPP, because it suppressed proteolysis and protected liver cells.

我们研究了连续低温灌注保存（CHPP）和戊茶碱（PTX）联合应用在无心跳供体（NHBD）肝移植中的作用。三个实验构成了这项研究。(1)我们从NHBD进行了猪肝移植。在本实验中，我们使用采购后的CHPP，并将PTX放入保存液中。因此，我们得出结论，CHPP与PTX是一个有用的程序，以改善从NHBD移植肝的功能。(2)本研究的目的是确定PTX预处理是否可以影响一氧化氮（NO）的释放，并在大鼠肝脏热缺血损伤有有益的影响。因此，PTX预处理增加了热缺血期间NO的产生。PTX对大鼠肝脏热缺血损伤有保护作用，NO对热缺血损伤有抑制作用。(3)本研究采用CHPP法保存大鼠肝脏，并探讨其最佳的灌流液。因此，UW-葡萄糖酸盐溶液被认为适用于CHPP，因为它抑制蛋白水解并保护肝细胞。

项目成果

Koichi Kozaki, Hiroto Egawa, CO.Esquivel: "Effects of pentoxifylline pretreatment on Kupffer cells in rat liver transplantation"Hepatology. 21(4). 1079-1082 (1995)

Koichi Kozaki，Hiroto Egawa，CO.Esquivel：“己酮可可碱预处理对大鼠肝移植中库普弗细胞的影响”肝病学。

Koichi Kozaki,et. al: "Usefulness of continuos hypothermic perfusion preservation for cadavericrenal grafts from non-heart-beating donors with prolonged warm ischemia time"Transplant.Proc. 29. 3586-3587 (1997)

小崎浩一等。

Koichi Kozaki, Masami Uchiyama, Takehiko Nemoto: "Usefulness of a combination of machine perfusion and pentoxifylline for porcine liver transplanation from non-heart-beating donors with prolonged hypotension"Transplant. Proc. 29. 3476-3477 (1997)

Koichi Kozaki、Masami Uchiyama、Takehiko Nemoto：“机器灌注和己酮可可碱组合在长期低血压的无心跳供体猪肝移植中的有用性”移植。

Koichi Kozaki, Masami Uchiyama, Takehiko Nemoto: "Usefulness of continuos hypothermic perfusion preservation for cadavericrenal grafts from non-heart-beating donors with prolonged warm ischemia time"Transplant. Proc. 29. 3586-3587 (1997)

Koichi Kozaki、Masami Uchiyama、Takehiko Nemoto：“连续低温灌注保存对于来自无心跳供体的尸体肾移植物的热缺血时间延长的有用性”移植。