Effect of mechanical stress loaded on the chondrocytes

机械应力对软骨细胞的影响

• 批准号: 08671699
• 负责人: FUKUDA Kanji
• 金额: $ 0.77万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671699/
• 关键词: chondrocytes; mechanical stress; nitric oxide; osteoarthritis; protein kinase C; 機械的ストレス; プロテオグリカン

Osteoarthrosis, a common pathway of joint deterioration, is caused by mechanical stress loaded on articular cartilage. We previously demonstrated the involvement of protein kinase C in the development of osteoarthritis in vitro. In the present study, we examined the effect of mechanical stress on chondrocyte metabolism and the activity of protein kinase C in vitro. Low frequency and magnitude of cyclic tensile stretch loaded on chondrocytes increased proteoglycan synthesis. However, high frequency and magnitude of stress decreased its synthesis. In this condition, activity of protein kinase C was reduced. These results suggest an involvement of protein kinase C in the stress mediated inhibition of proteoglycan synthesis. We also investigated the alteration of nitric oxide with mechanical stress. Both conditions caused the inhibition of No synthesis, thereby indicating the involvement of No in stress mediated alteration of the chondrocyte metabolism.

骨关节病是关节软骨受到机械应力而导致关节退化的常见途径。我们先前证明了蛋白激酶C参与体外骨关节炎的发展。在本研究中，我们研究了机械应力对软骨细胞代谢和蛋白激酶C活性的影响。低频率和幅度的周期性拉伸加载软骨细胞增加蛋白多糖的合成。然而，高频率和强度的应力降低其合成。在这种情况下，蛋白激酶C的活性降低。这些结果表明蛋白激酶C参与了应激介导的蛋白多糖合成抑制。我们还研究了一氧化氮与机械应力的变化。这两种情况都导致了NO合成的抑制，从而表明NO参与了应力介导的软骨细胞代谢的改变。

项目成果

田中清介, 福田寛二: "変形性股関節症" メジカルビュー社, 172 (1998)

Seisuke Tanaka、Kanji Fukuda：“髋关节骨关节炎”Medical View Publishing，172（1998）

K.Fukuda,et al: "Hyaluronic acid inhibits interleukin-1-induced superoxide anion in bovine chondrocytes" Inflamm Res. (in press). (1997)

K.Fukuda 等人：“透明质酸抑制牛软骨细胞中白细胞介素 1 诱导的超氧阴离子”Inflamm Res。

Fukuda K., et al: "Zonal difference of nitric oxide synthesis with interleukin-1 in bovine chondrocyte" Inflamm Res. 44. 434-437 (1995)

Fukuda K. 等人：“牛软骨细胞中白介素 1 与一氧化氮合成的区域差异”Inflamm Res。

Fukuda K., et al: "Interleukin-1 inhibits keratan sulfate production by rabbit chondrocytes : Possible role of prostaglandin E2." Inflamm Res. 44. 178-181 (1995)

Fukuda K. 等人：“Interleukin-1 抑制兔软骨细胞硫酸角质素的产生：前列腺素 E2 的可能作用。”

福田寛二,他: "Interleukin-1による軟骨破壊とガスメディエーター(総説)" 炎症. 16. 85-89 (1996)

Kanji Fukuda 等人：“Interleukin-1 和气体介质对软骨的破坏（评论）”炎症。 16. 85-89 (1996)