Induction of chondrocyte from human induced pluripotent stem cells

从iPS细胞诱导成软骨细胞是治疗骨关节疾病的重要手段。我们进行了1)通过iPS细胞的MSC产生来诱导iPS细胞的软骨细胞；2)MSCs的详细特征；3)细胞对机械应力的反应。这些实验表明，1)体外分化过程中的低氧处理是诱导MSCs的一个积极因素，2)诱导的MSCs具有典型的MSC特征，3)机械应力负荷导致控制细胞无分化的基因减少。本系列研究提供了一种从iPS细胞获得软骨细胞的有效方法。

Induction of chondrocyte from iPS cells is an important to treat osteoarticular disorders. We performed 1) induction of the chondrocyte from iPS cells via MSC production from iPS cells; 2) detailed characterization of the MSCs; 3) observation of cellular responses to mechanical stresses. These experiments elucidated that 1) hypoxic treatment during in vitro differentiation is a positive factor for induction of the MSCs, 2) induced MSCs possess typical MSC characteristics and 3) loading of the mechanical stress induce decreasing of the genes controlling undifferenciency of the cells. The present series of studies suggest an effective method to furnish the chondrocyte from iPS cells.