Mechanical stress loaded on articular cartilage explant

关节软骨外植体上加载的机械应力

• 批准号: 19591770
• 负责人: FUKUDA Kanji
• 金额: $ 3.08万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591770/
• 关键词: 軟骨; ストレス; 活性酸素; cartilage; chondrocyte; mechanical stress; regenerative medicine; reactive oxygen species; hyaluronan; mechanicalstress; tissue engineering; regemeratove medocine

Introduction : To repair the articular cartilage defect, cartilage explant is useful method. The key leading to the success of this regenerative medicine is to regulate the mechanical stress loaded on articular cartilage explant. Intraarticular hyaluronan (HA) injection is an effective treatment for cartilage degeneration. The purpose of this study is to determine the effect of mechanical stress loaded on the cartilage explant and possible usefulness of HA in this system.Methods : Mechanical compression was loaded on the bovine cartilage using the Biopress system. Proteoglycan (PG) and reactive oxygen species (ROS) synthesis were measured with [^<35>S] incorporation and fluorescent dye, respectively. Accumulation of peroxynitrite was determined with western blotting using nitrotyrosine antibody.Results : Mechanical compression inhibited PG synthesis and enhanced ROS. Externally added HA reversed stress-inhibited PG synthesis and attenuated ROS synthesis. HA also significantly decreased the expression of nitrotyrosine.Conclusions : Mechanical stress is essential factor to degradate the explanted cartilage and ROS play an important role. HA neutralized stress-enhanced ROS synthesis and resulted in the reverse of PG synthesis. These data suggest that HA plays anabolic effect as an antioxidant.

软骨移植是修复关节软骨缺损的有效方法。调节关节软骨外植体的机械应力是这种再生医学成功的关键。关节内注射透明质酸（HA）是治疗软骨变性的有效方法。本研究的目的是确定机械应力载荷对软骨外植体的影响以及HA在该系统中的可能用途。方法：采用Biopress系统对牛软骨施加机械压迫。蛋白聚糖（PG）和活性氧（ROS）的合成分别用[^<35>S]掺入法和荧光染料法测定。使用硝基酪氨酸抗体，用免疫印迹法测定过氧亚硝酸盐的积累。结果：机械压迫抑制PG合成，增强ROS。外部添加HA逆转应激抑制PG合成和减弱ROS合成。透明质酸也显著降低了硝基酪氨酸的表达。结论：机械应力是外植软骨降解的重要因素，ROS在其中起重要作用。透明质酸中和应激增强的ROS合成，导致PG合成逆转。这些数据表明透明质酸作为抗氧化剂具有合成代谢作用。

项目成果

Induction of mesenchymal progenitor cells with chondrogenic property from mouse-induced pluripotent stem cells.

• DOI: 10.1089/cell.2009.0086
• 发表时间: 2010-06
• 期刊: Cellular reprogramming
• 影响因子: 1.6
• 作者: Takeshi Teramura;Yuta Onodera;Toshihiro Mihara;Y. Hosoi;C. Hamanishi;Kanji Fukuda

間葉系幹細胞または軟骨細胞の製法ならびに発癌性の抑制方法.

间充质干细胞或软骨细胞的制造方法以及抑制致癌性的方法。

• 发表时间: 2009

Isolation and characterization of side population stem cells in articular synovial tissue.

• DOI: 10.1186/1471-2474-9-86
• 发表时间: 2008-06-12
• 期刊: BMC musculoskeletal disorders
• 影响因子: 2.3
• 作者: Teramura T;Fukuda K;Kurashimo S;Hosoi Y;Miki Y;Asada S;Hamanishi C
• 通讯作者: Hamanishi C

Differentiation diversity of mouse parthenogenetic embryonic stem cells in chimeric mice.

• DOI: 10.1016/j.theriogenology.2010.01.024
• 发表时间: 2010-07
• 期刊: Theriogenology
• 影响因子: 2.8
• 作者: Yuta Onodera;Takeshi Teramura;Madoka Ozawa;Toshiyuki Takehara;T. Mitani;M. Anzai;N. Sagawa;C. Hamanishi;Y. Hosoi;Kanji Fukuda

Potential existence of stem cells with multiple differentiation abilities to three different germ lineages in mouse neurospheres.

小鼠神经球中可能存在对三种不同种系具有多种分化能力的干细胞。

• 发表时间: 2009
• 期刊: Stem Cells Dev 18
• 作者: Takehara;T;Teramura;T;Onodera;Y;(他6名;2番目)
• 通讯作者: 2番目)