Neural plasticity relating to micturitinal disorder and its recovery in rats with injury to the central nervous system.

中枢神经系统损伤大鼠中与排尿障碍相关的神经可塑性及其恢复。

• 批准号: 08671791
• 负责人: SUGAYA Kimio
• 金额: $ 1.47万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671791/
• 关键词: decerebrate; micturition; dorsal system; spinothalamic tract; spinal cord; glutamate; glycine; 中枢神経; ラット; 排尿異常; 可塑性; c-fos; 脊髄損傷; 中枢神経系; 神経成長因子; ネコ; 後索; 膀胱内圧測定; Barrington核; HRP

1. Miclurihional disorder by partial decerebrae and its recovery. Partial inside or outside decerebrate of the frontal cortex induced large or small bladder volume, respectively. Twe weeks after partial decerebrate, however, bladder volume returned to the volume before decerebrate. Its recovery process could not be revealed by HRP study into the pontine micturition cenler, or distributions of positive neurons of nerve growth facter, c-fos, c-fos-mRNA, or NSE-mRNA.2. Micturitional disorder by dorsal funiculus injury and its reco very. In mid-brain decerebrate cats, dorsal funiculus injury did not influence the reflex micturition. In chronic cat with dorsal funiculus injury, single micturition volume decreased, and bladder sensation disappeared, but recovered 2 weeks after its injury. Although ascending pathways of bladder sensation are the dorsal system and spinotlialamic tract, the dorsal system may be the main pathway, and the spinothalamic tract may become to play a role of the dorsa … More l system when it is injured.3. Urinary retention by spinal cord injury and its recovery. Glutamate, aspartare, glycine and GABA in the lumbosacral spinal cord were measured by capillary electrophoresis. Intrathecal injection of glutamate increased glycine in the lumbosacral cord in rats. In acute spinal rats, glutamate and glycine increased in the lumbosacral cord, and intrathecal injection of glutamate receptor antagonist decreased glycine. Therefore, spinal glutamate neurons may be autoactivating neurons which are inhibited by the input from the upper central nervous system. When the input from the upper central nervous system disappears by spinal cord injury, autoactivating glutamate neurons may facilitate glycine neurons which may inhibit autonomic neurons and motoneurons except for Onuf's neurons innervating the external urethral sphincter muscle. In chronic spinal rats, glutamate and glycine in the lumbosacral cord decreased, suggesting that glycine neuronal activity may decrease by the decrease of glutamate neuronal activity, and that spinal hyper-reflexia may occur. Less

1.部分去脑致微神经元障碍及其康复。额叶皮质部分内侧去脑或外侧去脑分别引起膀胱体积增大或减小。然而，部分去脑后两周，膀胱容量恢复到去脑前的容量。结论：1.桥脑排尿中枢的HRP法、神经生长因子、c-fos、c-fos-mRNA、NSE-mRNA阳性神经元的分布均不能反映其恢复过程。脊髓背索损伤致排尿障碍及其康复。中脑去脑猫，背索损伤不影响反射性排尿。慢性脊髓损伤后，单次排尿量减少，膀胱感觉消失，2周后恢复。虽然膀胱感觉的上行通路是背侧系统和脊髓丘脑束，但背侧系统可能是主要通路，脊髓丘脑束可能成为背侧系统的作用 ...更多信息 l系统受到伤害时。脊髓损伤后尿潴留及其康复。用毛细管电泳法测定腰骶髓谷氨酸、甘氨酸、γ-氨基丁酸含量。鞘内注射谷氨酸增加大鼠腰骶髓中的甘氨酸。在急性脊髓损伤大鼠中，腰骶髓中的谷氨酸和甘氨酸增加，鞘内注射谷氨酸受体拮抗剂减少甘氨酸。因此，脊髓谷氨酸神经元可能是被来自上中枢神经系统的输入抑制的自激活神经元。当来自上中枢神经系统的输入因脊髓损伤而消失时，自激活的谷氨酸神经元可促进甘氨酸神经元，甘氨酸神经元可抑制除支配外尿道括约肌的Onuf神经元之外的自主神经元和运动神经元。在慢性脊髓损伤大鼠中，腰骶髓中的谷氨酸和甘氨酸减少，表明甘氨酸神经元活动可能通过谷氨酸神经元活动的减少而减少，并且可能发生脊髓反射亢进。少

项目成果

Sugaya,K.et al.: "Effect of injury to the dorsal funiculus of the thoracic spinal cord on micturition in decerebrate and freely-moving cats." Urologia Internationalis. (in press). (1999)

Sugaya，K.等人：“胸脊髓背索损伤对去大脑和自由移动猫排尿的影响。”

Sugaya, K.et al.: "Effect of injury to the dorsal funiculus of the thoracic spinal cord on micturition in decerebrate and freely-moving cate." Urologia Internationalis. (in press).

Sugaya, K.等人：“胸脊髓背索损伤对去大脑和自由移动动物排尿的影响。”