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Molecular biological studies of neurons in the central nervous system relating to the recovery from micturition disorders in rats with central nervous damage

中枢神经系统神经元与中枢神经损伤大鼠排尿障碍恢复相关的分子生物学研究

基本信息

批准号：
11671564
负责人：
SUGAYA Kimio
金额：
$ 2.18万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

1. Effects of intrathecal injections of tamsulosin (adrenergic α1A receptor antagonist) and naftopidil (adrenergicα1D receptor antagonist), on isovolumetric cystometry were investigated in rats with or without thoracic cord injury (TCI). Intrathecal injection of naftopidil transiently stopped bladder contraction, and decreased the amplitude of bladder contractions when large dose of naftopidil was administrated in rats with or without TCI. The volume of mRNA of adrenergic α1D in the lumbosacral cord did not change even after TCI. Therefore, adrenergic α1D receptors were located in both afferent and efferent limbs of the micturition reflex pathway, and serum catecholamines may also act on the spinal cord and facilitate micturition reflex.2. Cystometry and electrical myography of the external urethral sphincter were examined in TCI cats with or without autograft of the adrenal medulla into the sacral cord. In autografted cats, detrusorsphincter dyssynergia during bladder contraction improved, and tyrosine hydroxylase immuno-reactive cells were recognized in the sacral cord. Therefore, catecholamines projecting to the sacral cord may relax the urethra.3. Isovolumetric cystometry was performed before and after cerebral infarction (CI) in rats, and glutamate and glycine levels in the central nervous system were also measured. The frequency of bladder contractions increased 1-7 days after CI, but it returned to the control level 2-8 weeks after CI. Glutamate level in the cerebrum decreased, and glycine levels in the brainstem, cervicothoracic cord, and lumbosacral cord decreased after CI. However, glycine level in the lumbosacral cord returned to the control level 4-8 weeks after CI. Therefore, the frequency of bladder contractions may be depended on the glycine level in the lumbosacral cord.

1.观察鞘内注射肾上腺素能α1a受体拮抗剂坦索罗辛和肾上腺素能α1D受体拮抗剂萘托地尔对胸髓损伤大鼠等容膀胱测压的影响。无论有无TCI，鞘内注射大剂量萘哌地尔均可使膀胱收缩一过性停止，并降低膀胱收缩幅度。腰骶髓内肾上腺素能α1Dm RNA的体积在TCI后仍无明显变化。因此，肾上腺素能α1D受体位于排尿反射通路的传入和传出肢体，血清儿茶酚胺也可能作用于脊髓，促进排尿反射。对自体肾上腺髓质移植到骶髓的TCI猫进行了膀胱测压和外括约肌肌电检查。在自体移植猫中，膀胱收缩时逼尿肌括约肌协同失调得到改善，并在骶髓中识别出酪氨酸羟基酶免疫反应细胞。因此，儿茶酚胺投射到骶髓可能会松弛尿路。在大鼠脑梗塞(CI)前后进行等容膀胱测压，并测定中枢神经系统谷氨酸和甘氨酸水平。CI后1~7d膀胱收缩频率增加，CI后2~8周恢复至对照水平。脑梗塞后，脑内谷氨酸水平下降，脑干、颈胸段脊髓和腰骶段脊髓甘氨酸水平下降。而在脑梗塞后4-8周，腰骶髓内甘氨酸水平恢复到对照水平。因此，膀胱收缩的频率可能取决于腰骶髓中的甘氨酸水平。