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Isolation and characterization of rat urinary bladder cancer cell lines with different invasive activities

具有不同侵袭活性的大鼠膀胱癌细胞系的分离和表征

基本信息

批准号：
08671819
负责人：
NISHI Nozomu
金额：
$ 1.41万
依托单位：
Kagawa Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671819/
关键词：
Bladder cancer Invasion Metastasis Cell adhesion E-cadherin Catenin Epithelin Growth factor 増殖因子

项目摘要

We isolated, in vitro, spontaneous variants of the rat bladder tumor NBT-II cell line with a distinctive morphology. Of five sublines obtained, three (NBT-L1, L2a, and L2b) exhibited an elongated shape and moderate to high invasive activity in vitro. The other two sublines (NBT-T1 and T2) formed tight colonies and exhibited very low or negligible invasive activity. The contents of mRNAs coding for cell adhesion molecules (E-cadherin, a-catenin and b-catenin) were not correlated with the invasive activity of the cells. However, the expression level of the E-cadherin protein, but not those of catenins, was lower in invasive cells (NBT-L1, L2a and L2b) than in noninvasive cells (NBT-T1 and T2) . Analysis of mRNAs coding for several growth factors and their receptors showed that the transforming growth factor-alpha (TGF-alpha) mRNA content in invasive cells was higher than that in noninvasive cells, and that the content of epidermal growth factor receptor mRNA was low in NBT-T2. Although NBT-II is known to acquire a fibroblastic appearance and cell motility in response to several growth factors, the conditioned media of the invasive sublines hardly affected the morphology or motility of noninvasive cells. These results indicate that the decreased E-cadherin expression is a major cause of the transition from the noninvasive to the invasive phenotype of the bladder tumor cells, and that post-transcriptional process is important in the control of E-cadherin expression in the cells. These sublines may constitute useful models for studies on the malignant transformation of bladder tumors.

我们分离，在体外，自发变异的大鼠膀胱肿瘤NBT-II细胞系具有独特的形态。在获得的五个亚系中，三个（NBT-L1、L2 a和L2 b）在体外表现出细长的形状和中度至高度的侵袭活性。另外两个亚系（NBT-T1和T2）形成紧密的集落，并表现出非常低或可忽略的侵袭活性。编码细胞粘附分子（E-cadherin、a-catenin和b-catenin）的mRNA的含量与细胞的侵袭活性无关。然而，E-cadherin蛋白的表达水平，而不是连环蛋白的表达水平，在侵袭性细胞（NBT-L1，L2 a和L2 b）中低于非侵袭性细胞（NBT-T1和T2）。几种生长因子及其受体的mRNA编码的分析表明，转化生长因子-α（TGF-α）mRNA的含量在侵袭性细胞高于非侵袭性细胞，而表皮生长因子受体mRNA的含量在NBT-T2低。虽然NBT-II是已知的，以获得成纤维细胞的外观和细胞的运动性，在几个生长因子，条件培养基的侵入性亚系几乎没有影响的形态或运动性的非侵入性细胞。这些结果表明，E-cadherin表达的降低是膀胱肿瘤细胞从非侵袭性表型向侵袭性表型转变的主要原因，并且转录后过程在细胞中E-cadherin表达的控制中是重要的。这些亚系可能成为研究膀胱肿瘤恶性转化的有用模型。