In vivo observation of intrarenal microvessels and evaluation of the effect of exgenous high dose Angiotensin II forrenal microcirculation by a needle probe video microscope with a CCD camera.
利用针探针视频显微镜和CCD相机对肾内微血管进行体内观察并评价外源性高剂量血管紧张素II对肾微循环的影响。
基本信息
- 批准号:08671850
- 负责人:
- 金额:$ 1.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We visualized intrarenal microvessels by a needle-type video-microscope with a CCD camera (magnification =400). In 5 anesthetized dogs, the needle probe (O.D.=3.5 mm, length =6.5 mm) was introduced into the renal cortex or medulla through the renal capsule from the lateral renal border. Microscopic observation was discontinued when intrarenal bleeding occured (this was recognized by degradation of the image). Systemic Pressure and Renal blood flow after intraarterial infusion of angiotensin II changed significantly. MAP was elevated by angotensin II infusion from (incresed rate 18 (]SY.+-。[) 4 %) after 20 second later and remained high pressure for 3 minutes. Wheres RBF was decreased 25 (]SY.+-。[) 5 % significantly at 20 second which were significant difference, by intra-arterial infusion of 20ug angiotensin II,and returned to the basal level by 120 second. At the same time courese, in the visualized single nephron in vivo, Afferent and efferent arteriole began to be constricted distin … More ctly. At the first time afferent arteriole paticuraly seemmed to be trembled after boulas injection of angiotensin II,on the other hand it appeared vasomotion vigorously too, and the glomerus was shaked accordinaly. After trembling, afferent arteriole began to be constricted apparently at 10 second. The 60 second later, the diameter of afferent arteriole decresed 34% from 14.8 (]SY.+-。[) 4.5 mm, and returend to the basal diameter by 150 seconds. Wheres efferent arteriole was also constricted after 20ug AII infusion, but compeard with afferent arteriole, trembling or vasomotion was very restraind from angiotensin II effect. With the same time course, efferent artry was accordinaly constricted 36% from 13.9 (]SY.+-。[) 40mm, which had significant difference, and recovered by 150 seconds. Compered with affrent A constriction and efferent A constriction, These Arteries were constricted to minimam diameter at the almost same time. At the constricted diameter percentage, which was minimam diameter/maximum diameter at a mesured vessels, afferent Arteriole has a tendency, which was more constricted strongly than efferent arteriole. But two groups had no significant difference of T-test. Exgenous high dose Angiotensin II constricts both afferent arteriole and effernt arteriole in vivo. Less
我们用带CCD摄像机的针状视频显微镜(放大倍数=400)显示肾内微血管。在5只麻醉犬上,将直径为3.5 mm、长度为6.5 mm的针头从肾外侧缘经肾包膜送入肾皮质或髓质。当肾内出血发生时,停止显微镜观察(这是通过图像退化来识别的)。动脉内注射血管紧张素II后,体循环压力和肾血流量均有明显变化。静脉注射Angotensin II后,MAP明显升高,升高率为18(+-)。[4%]20秒后,保持高压3分钟。其中RBF降低25(]sy.+-。[]血管紧张素II 20ug动脉内注射,20s时显著下降5%,120s时恢复到基础水平。同时,在活体可见的单个肾单位中,…的传入和传出小动脉开始收缩更直截了当。在Boulas注射血管紧张素II后,传入小动脉第一次表现为颤动,另一方面也出现了强烈的血管运动,肾小球也相应地发生了抖动。震颤后10s,传入小动脉开始明显收缩。60s后,传入小动脉直径由14.8(Sy.+-)减小34%。[)4.5 mm,150秒后恢复到底径。注射AII 20ug后,传出小动脉收缩,但与传入小动脉相比,颤抖或血管运动明显抑制血管紧张素II的作用。在相同时间病程下,流出量由13.9士+-减少36%。[]40 mm,差异有统计学意义,恢复时间为150秒。与流入A收缩和传出A收缩相比,这些动脉几乎同时被收缩到最小直径。在测量血管的最小直径/最大直径处的收缩直径百分比处,传入小动脉有一种比传出小动脉更强烈的收缩趋势。但两组间t检验差异无统计学意义。外源性大剂量血管紧张素II在体内对传入小动脉和传出小动脉均有收缩作用。较少
项目成果
期刊论文数量(20)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Tokunori Yamamoto: "Bloocl velocity profole in the human renal artery By Doppler altvasound and thier relatsonsprip to athensclerosis" arteriosclenosis and thionbosis,and vascular bsolosy. Vol.1. 78-86 (1996)
Tokunori Yamamoto:“多普勒超声在人体肾动脉中的 Bloocl 速度剖面及其与动脉硬化、动脉硬化和血管硬化的关系。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
山本 徳則: "生体腎微小循環血流の光速度イメージング" 日本ME学会誌. 35・4. 235 (1997)
Norinori Yamamoto:“生物肾微循环血流的光速成像”日本 ME 学会杂志 35・4(1997 年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
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山本徳則: "ニードル型CCDプローブ法による生体循環への応用-腎微小循環を中心に-" 循環制御. 19・2(掲載予定). (1998)
山本纪典:“针型CCD探针法在生物循环中的应用-关注肾脏微循环-”循环控制(1998年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
山本徳則: "高速度ニードルプローブ生体顕微鏡を用いた腎微小循環のin vivo 血流速度解析" 第61回日本循環器学会総会. 61巻増刊. 100 (1997)
Norinori Yamamoto:“使用高速针探针生物显微镜进行肾脏微循环的体内血流速度分析”,日本循环学会第 61 届年会第 61 卷特刊(1997 年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
Tokunori Yamamoto, Hiroyoshi Tanaka etal.: "In-vivo hyperdynamic imaging in dog kidney microcirculation by High speed needle probe CCD microscope." Jounal of the American Society of Nephrology.Vol.8(suppl). 356A (1997)
Tokunori Yamamoto、Hiroyoshi Tanaka 等人:“通过高速针探针 CCD 显微镜对狗肾脏微循环进行体内超动态成像。”
- DOI:
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- 影响因子:0
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TANAKA Hiroyoshi其他文献
TANAKA Hiroyoshi的其他文献
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{{ truncateString('TANAKA Hiroyoshi', 18)}}的其他基金
Frictional performance of mixed metal soap greases
混合金属皂润滑脂的摩擦性能
- 批准号:
18560136 - 财政年份:2006
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Direct in vivo visualization of human renal microcirculation using less-invasive intravital videomicroscopy. -vascular structure and renal blood flow-
使用微创活体视频显微镜直接体内观察人肾微循环。
- 批准号:
12671569 - 财政年份:2000
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
In vivo vizualization of glomerular microcirculation microcirculation in AT1 a knock-out mice. -Role of anigiotensinII-
AT1 a 敲除小鼠肾小球微循环的体内可视化。
- 批准号:
10671506 - 财政年份:1998
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Newly development of CCD videomicroscope with a fine needle probe of side and straight angle mirror
新研制的带侧直角镜细针探头的CCD视频显微镜
- 批准号:
07557255 - 财政年份:1995
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)