In vivo vizualization of glomerular microcirculation microcirculation in AT1 a knock-out mice. -Role of anigiotensinII-

AT1 a 敲除小鼠肾小球微循环的体内可视化。

• 批准号: 10671506
• 负责人: TANAKA Hiroyoshi
• 金额: $ 1.66万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671506/
• 关键词: AT1 a knock out mice; AT1 antagonist; angiotensinII receptor; an intravital needle lens-probe videomicroscopy; renal microcirculation; 生体マウス糸球体微小循環; 糸球体血流速度ベクトル表示; ペンシルレンズCCD生体顕微鏡

We have recently developed an epoch-making technique to directly visualize a glomerular microcirculation of the kidney with an intravital needle type lens-probe videomicroscopy for a large animal (canine and human). In order to directly visualize a glomerular microcirculation for smaller animals such as rat and mouse, we improved the tip of probe of this system from a needle type lens-probe to a pencil type one.First, we succeeded as preliminary experiments in intravital direct vizualization of glomerular microcirculation in normal Wistar rats and streptorozocine induced diabetic rats under pre- and post intravenous administration of angiotensin II (AT1) antagonist (Losartan). In normal rats, the diameters of afferent and efferent arterioles were extended after administrating Losartan (1, 3 and 10 mg/kg) relating with dose dependency. In the diabetic rats, the diameters of afferent arterioles were not changed but those of efferent arterioles were extended as same as the normal rats after the administration of Losartan.Second, we could recently succeed in in vivo vizualization of glomerular microcirculation of mice and vector image processing of contarction of efferent arterioles of the glomeruli under the influence of angiotensin II administration.

我们最近开发了一种划时代的技术，直接可视化肾小球微循环的肾脏与活体针型镜头探头视频显微镜的大型动物（犬和人）。为了直接观察大鼠和小鼠等小型动物的肾小球微循环，我们将该系统的探头由针型透镜探头改进为铅笔型探头。我们成功地在正常Wistar大鼠和链脲佐菌素诱导的糖尿病大鼠中，在静脉内给予血管紧张素II（AT 1）前后进行了肾小球微循环的活体直接可视化的初步实验。拮抗剂（氯沙坦）。正常大鼠给予氯沙坦（1、3、10 mg/kg）后，传入和传出小动脉内径均明显增粗，并呈剂量依赖性。在糖尿病大鼠中，给予Losartan后，输入小动脉的直径没有改变，但输出小动脉的直径与正常大鼠相同。第二，我们最近成功地在体内可视化了小鼠肾小球微循环，并对血管紧张素II影响下的肾小球输出小动脉的收缩进行了矢量图像处理。

项目成果

Tong Wen Ou: "The value of three-dimensional helical computed tomographic angiography in the diagnosis of pelvi-ureteric junction obstruction caused by crossing vessels"BJU International. 83. 853-856 (1999)

欧童文：“三维螺旋CT血管造影对交叉血管所致盆腔输尿管交界部梗阻的诊断价值”北京和睦家大学国际。

山本徳則: "アンギオテンシンIIのin vivo糸球体毛細血管に対する影響" 日本腎臓学会誌. 40・4. 54 (1998)

Norinori Yamamoto：“血管紧张素II对体内肾小球毛细血管的影响”日本肾病学会杂志40・4.54（1998）。

山本徳則: "アンギオテンシンIIのin vivo糸球体毛細血管に対する影響"日本腎臓学会誌. 40. 107 (1998)

Norinori Yamamoto：“血管紧张素 II 对体内肾小球毛细血管的影响”日本肾病学会杂志 40. 107 (1998)。

Tong Wen Ou: "The value of three-dimensional helical computed tomographic angiography in the diagnosis of pelvi-ureteric junction obstruction caused by crossing vessels"B J U International. 83・7. 853-856 (1999)

欧童文：“三维螺旋计算机断层血管造影在诊断交叉血管引起的盆腔输尿管交界处梗阻中的价值”B J U International 83・7（1999）。

Tokunori Yamamoto: "In vivo glomerular microcirculation - Image analysis of glomerular non-flow and reflow phenomenon induced by vasoconstrictors in rat kidney using a newly developed needleprobe type CCD videomicroscopy"Journal of the American Society of

Tokunori Yamamoto：“体内肾小球微循环 - 使用新开发的针探针型 CCD 视频显微镜对大鼠肾脏血管收缩剂诱导的肾小球非流动和回流现象进行图像分析”美国学会杂志