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Direct in vivo visualization of human renal microcirculation using less-invasive intravital videomicroscopy. -vascular structure and renal blood flow-

使用微创活体视频显微镜直接体内观察人肾微循环。

基本信息

批准号：
12671569
负责人：
TANAKA Hiroyoshi
金额：
$ 2.5万
依托单位：
KAWASAKI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Several technical approaches for the evaluation of renal microcirculation have been developed Although each methodology possesses unique advantages, their common disadvantage is in the need for substantial manipulation on the kidney that might alter the renal microvascular responsiveness.To circumvent this drawback of previous approaches to renal microcirculation, we have developed a pencil-lens probe ($ 1mm, length : 25cm, weight 160g) with the charge-coupled divice (CCD) intravital videomicroscopic system (spatial resolution : 0.86um, time resolution : 30 frame/sec), which combines the characteristics ofmicroscopy and endoscopy. This system allowed us to perform less- invasive evaluation of both the renal structure and microcirculation approach to laparoscopic hole and open operation. Furthermore, real-time imaging of afferent and efferent arterioles, as well as glomeruli can be continuously assessed, thus permitting the functional characterization of microcirculation. A line segment was set along the glomerular capillaries and then a spatiotemporal image was constructed along the segment. The angle of stripped pattern in the spatiotempolar image, which is related to the erythrocyte velocity, was applied to compute erythrocyte velocity vectors as previously reported from Ogasawara et al group. Motion analysis of sequential images yields data on velocity of erythrocytes and leukocytes in glomeruli. In conclusion, this less-invasive technique will reveal as yet unknown field of renal microcirculation.

肾微循环的检测方法有多种，虽然每种方法都有其独特的优点，但它们的共同缺点是需要对肾脏进行实质性的操作，从而可能改变肾微血管的反应性。为了克服以往肾微循环检测方法的缺点，我们研制了一种双透镜探头（$1mm，长：25 cm，重：160 g）与电荷耦合器件（CCD）活体视频显微镜系统（空间分辨率：0.86um，时间分辨率：30帧/秒），结合了显微镜和内窥镜的特点。该系统使我们能够对腹腔镜孔和开放手术的肾脏结构和微循环进行微创评估。此外，可以连续评估传入和传出小动脉以及肾小球的实时成像，从而允许微循环的功能表征。沿肾小球毛细血管沿着设置一条线段，然后沿该线段沿着构建时空图像。根据Ogasawara等人的研究结果，利用与红细胞运动速度有关的时空极像中条纹图案的角度来计算红细胞运动速度矢量。运动分析的顺序图像产生的数据的速度的红细胞和白细胞在肾小球。总之，这种微创技术将揭示肾脏微循环的未知领域。