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Newly development of CCD videomicroscope with a fine needle probe of side and straight angle mirror

新研制的带侧直角镜细针探头的CCD视频显微镜

基本信息

批准号：
07557255
负责人：
TANAKA Hiroyoshi
金额：
$ 0.51万
依托单位：
Kawasaki Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

To visualize the human renal microvessels, we have developed a fine needle type CCD microscope. The needle probe (diameter : 3.5 mm, length : 65 mm) contains a gradient index (GRIN) lens, surrounded by an annular light guide. Two access methods of the needle probe to superficial nephrons were applied. The first one was direct access to a kidney. The probe (angle of viewing field : 0 deg) was introduced into the superficial renal cortex through the renal capsule at the lateral renal border during operation (renal injury=1.renal cancer=3, ureteral cancer=1 ; 5 Kidneys in total) under general anesthesia. In the second method, the needle probe (angle of viewing field : 90 deg) was introduced percutaneously into the intra-renal tissue through nephrostomy during nephrostomy tube exchange (postoperative uterine cancer=2, postoperative rectal cancer=1 ; 4 kidneys in total) under no anesthesia. We injected the dye (Indicocarmine, 20 mg) into the intravenous root at 4-5 minutes before observation of intra-renal microvessels. The dye was excreted from a glomerular capsule to a proximal tubulus. The distal tubulus was also visualized by dye injection. Some bubbles of about 20 mm in diameter were observed in these tubules and were moved toward downstream by the peristalsis of the tubules. We could clearly visualize the in vivo human intrarenal vessels (afferent arterioles, glomerular capillary loops, efferent arterioles, peritubular networks, and vasa recta) and proximal and distal tubulus with the dye injected to intravenous root by our novel system.

为了观察人肾微血管，我们研制了一种细针型CCD显微镜。探针（直径：3.5 mm，长度：65 mm）包含梯度折射率（GRIN）透镜，由环形光导包围。采用两种方法将针探针插入浅表肾单位。第一个是直接进入肾脏。在全身麻醉下，将探头（视野角：0度）在手术过程中（肾损伤= 1，肾癌=3，输尿管癌=1 ;共5个肾）通过肾外侧缘处的肾包膜引入浅表肾皮质。在第二种方法中，在无麻醉的情况下，在肾造口管交换期间（术后子宫癌=2例，术后直肠癌=1例;共4个肾脏），通过肾造口将针探头（视野角：90度）经皮引入肾内组织。我们在观察肾内微血管前4-5分钟将染料（Indicocarmine，20 mg）注射到静脉根部。染料从肾小球囊分泌到近端小管。还通过染料注射使远端小管可视化。在这些小管中观察到一些直径约20 mm的气泡，并通过小管的膨胀向下游移动。我们可以清楚地显示在体内的人肾内血管（输入小动脉，肾小球毛细血管袢，输出小动脉，管周网络，和直血管）和近端和远端小管与染料注射到静脉根部，我们的新系统。