Detection of the regulatory factor for decidualization with mRNA differential display method

mRNA差异显示法检测蜕膜化调控因子

• 批准号: 08671912
• 负责人: SHIGETA Hiroyuki
• 金额: $ 1.47万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671912/
• 关键词: endometrium; decidua; growth factor; differential display; GBP-2; nidogen-2; diffrential display; 遺伝子発現

Many factors could be involved in the endometrial differentiation. To investigate the possible factors that regulate endometrial differentiation, we first studied the mRNA expression of transforming growth factor-alpha, gonadotropin-releasing hormone, keratinocyte growth factor, and platelet-derived growth factor in the human endometrium. The results showed that all these mRNAs existed in endometrium and had biological effects to it such as cell proliferation and stimulation of hCG secretion. We next examined the existance of estrogen-related receptor alpha in mouse uterus. We found that this orphan receptor did exist in mouse uterus and its expression was stimulated by estrogen.To find the new factor which is involved in human endometrial differentiation, we employed mRNA differential display method. We found two proteins, guanylate- binding protein-2 (GBP-2) and nidogen-2, which showed increasing expression pattern from proliferative phase to secretory phase. Because the expression of GBP-2 is known to be stimulated by interferon, we need to pay attention to the effect of interferon in endometrium. Nidogen-2 is a constituent of basement membrane and therefore may have a significant role for the assembly of the basement membrane in human endometrium.

子宫内膜分化与多种因素有关。为了探讨调节子宫内膜分化的可能因素，我们首先研究了人子宫内膜中转化生长因子- α、促性腺激素释放激素、角化细胞生长因子和血小板源性生长因子的mRNA表达。结果表明，这些mrna均存在于子宫内膜中，并对子宫内膜具有细胞增殖、刺激hCG分泌等生物学效应。接下来，我们检测了小鼠子宫中雌激素相关受体α的存在。我们发现该孤儿受体在小鼠子宫中确实存在，并在雌激素的刺激下表达。为了寻找参与人子宫内膜分化的新因子，我们采用mRNA差异显示法。我们发现鸟苷酸结合蛋白-2 （GBP-2）和nidogen-2从增殖期到分泌期表达增加。因为已知GBP-2的表达会受到干扰素的刺激，所以我们需要关注干扰素在子宫内膜中的作用。Nidogen-2是基底膜的组成成分，因此可能对人子宫内膜基底膜的组装有重要作用。

项目成果

Matsui H, Taga M, Kurogi K, Minaguchi H: "Gene expression of keratinocyte growth factor and its receptor in the human endometrium/decidua and chorionic villi." Endocrine J. 44. 867-871 (1997)

Matsui H、Taga M、Kurogi K、Minaguchi H：“角化细胞生长因子及其受体在人子宫内膜/蜕膜和绒毛膜绒毛中的基因表达。”

Matsui H: "Gene expression of Keratinocyte growth factor and its receptor in the human endometrium/decidua and chorionic villi." Endocrine J.44. 867-871 (1997)

Matsui H：“角化细胞生长因子及其受体在人子宫内膜/蜕膜和绒毛膜绒毛中的基因表达。”

Saji, M., Taga, M., Matsui, H., Suyama, K., Kurogi, K., Minaguchi, H.: "Gene expression and specific binding of platelet-derived growth factor and its effect on DNA synthesis in human decidual cells." Mol.Cell.Endocrinol. 132. 73-80 (1997)

Saji, M.、Taga, M.、Matsui, H.、Suyama, K.、Kurogi, K.、Minaguchi, H.：“血小板衍生生长因子的基因表达和特异性结合及其对人类 DNA 合成的影响

Ikeda M: "Gene expression of gonadotropin-releasing hormone, but not its receptor, in human endometrium and decidua" Mol Cell. Endocrinol. 135. 165-168 (1997)

Ikeda M：“人类子宫内膜和蜕膜中促性腺激素释放激素的基因表达，但不是其受体”Mol Cell。

Michiyoshi Taga: "Gena Espression of Transforming Growth Factor-α in Human Endomctrium During Decidua lization" J Assist Reprod Gen. 14. 218-222 (1997)

Michiyoshi Taga：“蜕膜化过程中人类内分泌中转化生长因子-α 的基因表达”J Assist Reprod Gen. 14. 218-222 (1997)