The production and regulation of vascular endothelial growth factor by uterine endometrium, decidua and placental tissues.

子宫内膜、蜕膜和胎盘组织血管内皮生长因子的产生和调节。

• 批准号: 13671733
• 负责人: MIYAKAWA Isao
• 金额: $ 2.3万
• 依托单位: Oita University (2004)大分医科大学 (2001-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671733/
• 关键词: Vascular endothelial growth factor; Endometrial stromal cells; Amniotic cells; 血管新生

Our objective was to clarify the physiological role of vascular endothelial growth factor(VEGF) by endometrial stromal cells(ESC) and amnion-derived(WISH) cells. ESC were cultured, and VEGF production was increased by epidermal growth factor(EGF) and was decreased by interferon(IFN)-γ in a dose-dependent manner. VEGF production and VEGF mRNA expression were also increased by medroxyprogesterone(MPA) and cyclic AMP during in vitro decidualization. IP-10 production and IP-10 mRNA expression were increased by interleukin-1β or tumor necrosis factor-α.WISH cells were cultured, and VEGF production was increased by EGF or insulin-like growth factor(IGF)-1 in a dose-dependent manner. The activation of MAP kinase and akt, which is phosphorylated by PI 3-kinase, were detected by western blot analysis. VEGF production was significantly decreased following pretreatment with MEK inhibitor(U0126) or PI 3-kinase inhibitor(wortmannin), and then treated with EGF or IGF-1.It is suggested that VEGF may contribute to the neovascularization and proliferation of the uterine endometrium, placenta and gestational tissue.

我们的目的是阐明血管内皮生长因子(VEGF)在子宫内膜间质细胞(ESC)和羊膜来源细胞(WISH)中的生理作用。表皮生长因子可促进ESC产生血管内皮生长因子，而干扰素-γ则呈剂量依赖性地抑制血管内皮生长因子的产生。体外蜕膜形成过程中，甲羟孕酮(MPA)和环磷酸腺苷(CAMP)也可促进血管内皮生长因子(VEGF)的产生和表达。IL-1β或肿瘤坏死因子-α可促进WISH细胞产生IP-10和表达IP-10mRNA，并呈剂量依赖性。免疫印迹法检测MAP和PI3-激酶磷酸化的AKT的活性。预先给予MEK抑制剂(U0126)或PI 3-激酶抑制剂(Wortmannin)，再用EGF或IGF-1处理后，VEGF的产生明显减少。提示，VEGF可能参与子宫内膜、胎盘和妊娠组织的新生血管和增殖。

项目成果

Survivin expression correlates with clinical stage, histological grade, invasive behavior and survival rate in endometrial carcinoma.

• DOI: 10.1016/j.canlet.2013.02.027
• 发表时间: 2002
• 期刊: Cancer letters
• 影响因子: 9.7
• 作者: N. Takai;T. Miyazaki;M. Nishida;K. Nasu;I. Miyakawa

Synergistic effects of interleukin (IL)-1α and ceramide analogue on the production of IL-6, IL-8 and macrophage colony-stimulating

白介素 (IL)-1α 和神经酰胺类似物对 IL-6、IL-8 产生和巨噬细胞集落刺激的协同作用

• 发表时间: 2004
• 期刊: Fertil Steril 82・Supple 3
• 作者: Yasushi Kawano;Junichiro Fukuda;Kaei Nasu;et al.
• 通讯作者: et al.

Synergistic effects of interleukin (IL)-1α and ceramide analogue on the production of IL-6, IL-8 and macrophage colony-stimulating factor by endometrial stromal cells.

白细胞介素 (IL)-1α 和神经酰胺类似物对子宫内膜基质细胞产生 IL-6、IL-8 和巨噬细胞集落刺激因子的协同作用。

• 发表时间: 2004
• 期刊: Fertil Steril 82・Supple 3
• 作者: Yasushi Kawano;Junichiro Fukuda;Kaei Nasu;et al.
• 通讯作者: et al.

Clinical relevance of Elf-1 expression in endometrial carcinoma.

Elf-1 表达在子宫内膜癌中的临床相关性。

• 发表时间: 2003
• 期刊: Gynecol Oncol 89・3
• 作者: Noriyuki Takai;Tami Miyazaki;Masakazu Nishida;et al.
• 通讯作者: et al.

The significance of Elf-1 expression in epithelial ovarian carcinoma.

• DOI: 10.3892/ijmm.12.3.349
• 发表时间: 2003-09
• 期刊: International journal of molecular medicine
• 影响因子: 5.4
• 作者: N. Takai;T. Miyazaki;M. Nishida;K. Nasu;I. Miyakawa