Study on human cone system using long -and middle-wavelength sensitive cone electroretinagram and short-wavelength sensitive cone ERG

长中波长敏感锥体视网膜电图和短波长敏感锥体ERG的人体锥体系统研究

• 批准号: 08672015
• 负责人: HORIGUCHI Masayuki
• 金额: $ 1.28万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672015/
• 关键词: long- and middle-wavelength sensitive cone electroretinogram; short-wavelenghth sensitive cone electroretinogram; aging, developement, retinal disease; 網膜疾患; 発達; on双曲細胞; off双曲細胞; 青錘体ERG; 赤緑錘体ERG; 成長

In this study, we recorded long- and middle-wavelength sensitive cone electroretinogram (LM-cone ERG) and short-wavelength sensitive cone ERG (S-cone ERG) from normal subjects to investigate development and aging of the cone systems. Further, LM-and S-cone ERGs were recorded from patients with retinal diseases. LM-cone d-wave showed earlier development than other components, suggesting tht on- and off-mechanisms of the rmenbrane potential in the retinal neurons have different processes of development. LM cone ERG a-wave revealed greater age-related reduction than b-wave, indicating vulnerability of off-bipolar cell to aging. S-cone ERG showed slower developement and earlier age-related reduction than LM-cone ERG.In congetital stationally night blindness (CSNB), LM cone ERG b-wave diminished and S-cone ERG was gone, suggesting dysfunction of on-bipolar cells. However, in unilateral aquired night blindness, a new disease reported by Fishman, we found reduced LM-cone b-wave with preserved S-cone ERG.The mechanism of on-bipolar cell dysfunction is different between these diseases.

本研究通过记录正常人视锥系统的长、中波长敏感视锥电图（LM-cone ERG）和短波敏感视锥电图（S-cone ERG）来观察视锥系统的发育和老化情况。此外，还记录了视网膜疾病患者的lm -cone和S-cone eeg。脑视锥d波发育较其他成分早，提示视网膜神经元膜电位的开启机制和关闭机制具有不同的发育过程。LM锥ERG a波比b波显示出更大的年龄相关性下降，表明非双极细胞易受衰老影响。s -锥体ERG比lm -锥体ERG发育更慢，与年龄相关的下降更早。充血性静止性夜盲症（CSNB）时，左脑锥体ERG b波减弱，s锥体ERG消失，提示双极细胞功能障碍。然而，在Fishman报道的单侧获得性夜盲症中，我们发现左脑锥体b波减少，s锥体ERG保留。双极细胞功能障碍的机制在这些疾病之间是不同的。

项目成果

Miyake Y.Horiguchi M.et al.: "Electrophysiological findings in patients with Oguchis disease" Jpn J Ophthalmol. 40. 511-519 (1997)

Miyake Y.Horiguchi M.等人：“Oguchis 病患者的电生理学发现”Jpn J Ophthalmol。

Miyake.Y.Horiguchi.M.et al: "Electrophysiological findings in patients with Oguchi's disease" Jpn J Ophthalmol. 40. 511-519 (1997)

Miyake.Y.Horiguchi.M.等人：“大口氏病患者的电生理学发现”Jpn J Ophamol。

Miyake Y, Horiguchi M et al.: "Occult macular dystrophy" Am J Ophthalmol. 122. 644-653 (1996)

Miyake Y、Horiguchi M 等人：“隐匿性黄斑营养不良”Am J Ophamol。

堀口 正之、三宅 養三 他: "30Hz narrow bandpass filterを用いたportable ERG記録装置" 眼臨. 91. 148-150 (1997)

Masayuki Horiguchi、Yozo Miyake 等人：“使用 30Hz 窄带通滤波器的便携式 ERG 记录设备”意见。 91. 148-150 (1997)

Miyake Y.: "Horiguch.M et al Ocoult macular dystrophy" Am.J.Ophthalmol. 122. 644-653 (1966)

Miyake Y.：“Horiguch.M 等人 Ocoult 黄斑营养不良”Am.J.Ophthalmol。