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A Study on the Formation of Microbial Biofilms in the Infected Root Canals with refractory apical periodontitis, and on the Effect of Medicine against Biofilms

难治性根尖周炎感染根管内微生物生物膜的形成及药物抗生物膜的研究

基本信息

批准号：
08672215
负责人：
TAKIZAWA Hisashi
金额：
$ 1.41万
依托单位：
Tsurumi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672215/
关键词：
Refractory Apical Periodontitis Microorganism Biofilm Glycocalyx Effective Medicine 難治根光性歯周炎

项目摘要

173 microorganisms were isolated from 40 infected root canals with refractory apical periodontitis. Among them, Pseudomonas aeruginosa (P.s.), Staphylococcus aureus(S.a.), Bacillus subtilis(B.s.), Enterococcus faecalis(E.t.) and Candida albicans(C.a.) were used. They were adhered to each cell disk and cultured with two kinds of method. One was set in multiplate with TS Broth (method 1), and another was hanged in each small bottle (method 2). As the culture medium of method 2, TS Broth, TS Broth with plasma and saline with plasma were used . Culture periods were 3,7 andl4 days in 37ﾟC.Then each cell disk was observed with SEM.The substances like glycocalyx were observed in each microorganism except E.f. andC.a. (method 1), and except C.a.(method 2), increasing with the extension of culture period. Besides those substances increased in the medium adding plasma.As the medicine against microorganism with biofllm, FC (100%, 10%, 1%), J(100%, 10%, 1%), NaClO(l0%, 5%, 1%) and oxidative potential water were used. After cultivation in TS Broth with plasma for 7 days, each microorganism was immersed in each medicine for 15 minutes and cultured again for 24 and 48 hours. NaClO and oxidative potential water were only used for S.a..Undiluted solutions of FC and J were effective in each microorganism, but diluted solutions reduced the effect. The effect of NaClO also reduced in low concentration. Oxidative potential water was inefficient. Microorganisms can form bioflims in the apical portion of root canal with refractory apical periodontitis. So it seems to be difficult to supply sufficient medicine. In future it will be necessary to study about the method for destruction or melting of bioflims.

从40个难治性根尖周炎感染根管中分离到173株微生物。其中，铜绿假单胞菌（P.s.），金黄色葡萄球菌（S.a.），枯草芽孢杆菌（B.s.），粪肠球菌（E.t.）和白色念珠菌（C.a.）用了将其贴附于每个细胞盘上，用两种方法进行培养。一个置于含有TS肉汤的多板中（方法1），另一个悬挂在每个小瓶中（方法2）。作为方法2的培养基，使用TS肉汤、含有血浆的TS肉汤和含有血浆的盐水。在37 ℃下培养3、7、14天后，用扫描电镜观察细胞圆片，除E. f.和C.a.（方法1），并且除了C. a.（方法2），随着培养时间的延长而增加。除上述物质外，在培养基中加入血浆后，生物膜的抑菌药物为FC（100%，10%，1%）、J（100%，10%，1%）、NaClO（10%，5%，1%）和氧化电位水。在含有血浆的TS肉汤中培养7天后，将每种微生物浸入每种药物中15分钟，并再次培养24和48小时。NaClO和氧化电位水仅用于S.a.。FC和J的未稀释溶液对每种微生物都有效，但稀释溶液降低了效果。NaClO在低浓度时对细胞的影响也减弱。氧化电位水是无效的。难治性根尖周炎根管内微生物可形成生物膜。因此，似乎很难提供足够的药物。今后有必要研究生物膜的破坏或融化方法。