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Experimental studies on successive changes of the microvascular architecture during the period of bone healing in extracted sockets using human dura mater.

使用人硬脑膜拔出牙槽骨愈合期间微血管结构连续变化的实验研究。

基本信息

批准号：
08672271
负责人：
SUWA Fumihiko
金额：
$ 1.15万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672271/
关键词：
Guided bone regeneration Microvascularization Osteogenesis Repairing of wound Dura Mater Alveolar bone Augmentation GBR Bone Monkey

项目摘要

It is necessary to control the absorption of alveolar bone after tooth extraction for prosthodontic treatment. The guided bone regeneration (GBR) method has been reported on the application to the extracted tooth socket, and how to use an absorbable membrane to reduce the patient's pain. This study investigated the contribution of human dura mater to microvascularization and osteogenetic changes in extracted sockets by SEM observation.Materials and MethodsThe upper incisors in Macaca fuscota were bilaterally extracted, and the human dura mater was applied and covered one of the sockets. The other side was the control. After application, the gingivoperiosteal flap was carefully sutured. At 1,2,4,6 and 8 weeks after postoperatively, microvascular casts prepared by the plastic injection method were examined by SEM.At 1 week, both site sockets were filled with the newly-formed sinusoidal capillaries which elongated from the socket wall ; no bone formation was observed. At 2 weeks in the experimental site, the newly-formed microvascularization was denser than the control. At 4 weeks, the bottom half of the both site sockets were filled with newly-formed bone trabeculae, but in the experimental site, the new bone trabeculae was denser. At 6 weeks, the both site sockets were filled with new bone trabeculae. However, we observed a shallow concave at the top of each socket. At 8 weeks, in the experimental site, the extracted socket was filled with the new bone trabeculae, and the edge of the socket was flat. While in the control site, the shallow concave was still observable.We conclude that the human dura mater contributes to the osseous healing of the socket left by extraction and prevents the absorption of alveolar bone.

拔牙后牙槽骨吸收的控制是口腔修复治疗的必要条件。已经报道了引导骨再生（GBR）方法在拔牙窝中的应用，以及如何使用可吸收膜来减轻患者的疼痛。本研究探讨了人类硬脑膜的贡献，微血管和成骨的变化，在提取插座通过SEM observation.Materials和MethodsThe上切牙在恒河猴fuscota双侧提取，和人类硬脑膜应用和覆盖的一个插座。另一边是控制。应用后，仔细缝合牙龈骨膜瓣。术后1、2、4、6、8周，用塑料注射法制作微血管铸型，扫描电镜观察，1周时，两个部位牙槽窝均被新生的窦状毛细血管充满，从牙槽窝壁延伸而出，未见骨形成。2周时，实验部位新生的微血管较对照组密集。4周时，两个部位骨槽的下半部分被新形成的骨小梁填充，但在实验部位，新骨小梁更密集。6周时，两个部位的骨槽均充满了新的骨小梁。然而，我们在每个插座的顶部观察到一个浅凹。8周时，实验区拔牙窝内新生骨小梁充填，牙槽边缘平整。而对照组牙槽窝仍可见浅凹，提示硬脑膜有助于拔牙后牙槽窝的骨性愈合，并阻止牙槽骨的吸收。