Pothogenic mechanism of oral severe infection : Effect of oxidativc stress on neutrophil apoptosis.

口腔严重感染的致病机制：氧化应激对中性粒细胞凋亡的影响。

• 批准号: 08672333
• 负责人: IWASE Masayasu
• 金额: $ 1.41万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672333/
• 关键词: Neutrophil; Apoptosis; Fas antigen; Fas ligand; Auute inflammation; Differentiotion; Cytokine; Chemoattractant; 感染症; 活性酸素

1.Fas and Fas ligand were expressed on neutrophils consitutively. The expression of Fas increased in postsurgical acute inflammation, whereas the expression of Fas ligand on neutrophils was constant during observation. The levels of soluble Fas antigen in serum were also unchangeable postsurgically. Soluble Fas ligand levels were undetectable in any serum samples in this study.2.A chemoattractant such as FMLP,PAF,LTB4 and IL-8 did not change the expression of Fas/Fas ligand of neutrophils. Apoptosis of neutrophils exhibited the increase/decrease by addition of the chemoattractants. GTP protein inhibitor and tyrosin kinase inhibitor reduced the mode.3.The-expression of Fas antigen on HL-60 cells was increased with a differentiation inducing factor including DMSO,RA and 1,25 (OH)_2 VD_<3・> On the contrary, bcl-2 protein in HL-60 cells was decreased by treatment of the factor. These results suggest that differentiated HL-60 cells may become susceptible to Fas/Fas ligand mediated apoptosis.4.The expression of Fas antigen on HL-60 cells was also increased with INF-gamma or TNF-alpha. These results indicate that cytokine such as INF-gamma or TNF-alpha regulates a susceptibility of Fas mediated cell death.

1.Fas和Fas配体在中性粒细胞上组成表达。术后急性炎症中Fas表达增加，而观察期间中性粒细胞上Fas配体表达不变。术后血清可溶性Fas抗原水平也没有变化。可溶性Fas配体水平在本研究中未检测到任何血清样本。FMLP、PAF、LTB4、IL-8等趋化剂均未改变中性粒细胞Fas/Fas配体的表达。加入化学引诱剂后，中性粒细胞的凋亡呈增加/减少的趋势。GTP蛋白抑制剂和酪氨酸激酶抑制剂降低了模型。在DMSO、RA和1,25 (OH)_2 VD_<3·>诱导因子的作用下，HL-60细胞Fas抗原的表达增加，而bcl-2蛋白的表达则降低。这些结果提示分化后的HL-60细胞可能易受Fas/Fas配体介导的凋亡影响。nf - γ或tnf - α均可增加HL-60细胞上Fas抗原的表达。这些结果表明，细胞因子如inf - γ或tnf - α调节Fas介导的细胞死亡的易感性。

项目成果

M Ohashi, M Iwase et al: "Upregalation of Fas/Fas ligand expression by IFN-γ or TNF-α on HL-60 cells" MOL BIOL CELL. 8. 249 (1997)

M Ohashi、M Iwase 等人：“IFN-γ 或 TNF-α 在 HL-60 细胞上上调 Fas/Fas 配体表达”MOL BIOL CELL。

Ohashi M, et al: "Expression of Fas/Fas ligand and bel-2 on differentiated HL-60 cells." Inflamm Res. 46・3. S214- (1997)

Ohashi M 等人：“Fas/Fas 配体和 bel-2 在分化的 HL-60 细胞上的表达”。 Inflamm Res 46·3。

Ohashi M, et al: "Upregulation of Fas/Fas ligand expression by IFN-γ or TNF-α on HL-60 cell." Mol Biol Cell. 8. 249- (1997)

Ohashi M 等人：“IFN-γ 或 TNF-α 对 HL-60 细胞上 Fas/Fas 配体表达的上调”。8. 249-(1997)。

Iwase M, et al: "Changes of Fas antigen and Fas ligand on perspheral blood neufrophils and in serum in acute inflammation." Inflamm Res. 46・3. S214- (1997)

Iwase M 等人：“急性炎症中外周血嗜中性粒细胞和血清中 Fas 抗原和 Fas 配体的变化”，Inflamm Res 46·3。

Iwase M., et al: "Changes neutrophil elastase in postoperative inflammation." J Jpn Stomatol Soc. 46-2. 117-121 (1997)

Iwase M. 等人：“术后炎症中中性粒细胞弹性蛋白酶的变化。”