Pothogenic mechanism of oral severe infection : Effect of oxidativc stress on neutrophil apoptosis.

1. Fas和Fas Ligand在中性哲学上表达了合理的观点。在手术后急性炎症中，Fas的表达增加了，而在观察期间，Fas Ligand对中性药物的表达不断增加。血清中的溶解性反性水平在手术后也是不可改变的。在本研究中，任何血清样本中的溶解性Fas配体水平都是可量化的。2.一种化学催化剂，如FMLP、PAF、LTB 4和IL-8，并没有改变Fas/Fas配体的表达。营养素的凋亡被揭示为化学吸附剂的添加剂的增加/减少。GTP蛋白抑制剂和酪氨酸激酶抑制剂减少了模式。3. HL-60细胞中的假抗原表达，包括DMSO、RA和1，25 （OH）_2VD_<3·>在相反的情况下，HL-60细胞中的Bcl-2蛋白被去除了该因子的治疗。这些结果建议不同的HL-60细胞可能会被怀疑为Fas/Fas配体和介导的药物。4. HL-60细胞上的Fas抗原的表达也被INF-gamma或TNF-alpha所增加。这些结果表明，细胞因子（如INF-伽马或TNF-α）调节为癌介导细胞死亡的可能性。

1.Fas and Fas ligand were expressed on neutrophils consitutively. The expression of Fas increased in postsurgical acute inflammation, whereas the expression of Fas ligand on neutrophils was constant during observation. The levels of soluble Fas antigen in serum were also unchangeable postsurgically. Soluble Fas ligand levels were undetectable in any serum samples in this study.2.A chemoattractant such as FMLP,PAF,LTB4 and IL-8 did not change the expression of Fas/Fas ligand of neutrophils. Apoptosis of neutrophils exhibited the increase/decrease by addition of the chemoattractants. GTP protein inhibitor and tyrosin kinase inhibitor reduced the mode.3.The-expression of Fas antigen on HL-60 cells was increased with a differentiation inducing factor including DMSO,RA and 1,25 (OH)_2 VD_<3・> On the contrary, bcl-2 protein in HL-60 cells was decreased by treatment of the factor. These results suggest that differentiated HL-60 cells may become susceptible to Fas/Fas ligand mediated apoptosis.4.The expression of Fas antigen on HL-60 cells was also increased with INF-gamma or TNF-alpha. These results indicate that cytokine such as INF-gamma or TNF-alpha regulates a susceptibility of Fas mediated cell death.