MECHANISM OF PROLONGED INFLAMMATION IN THE ORAL:EFFECT OF NEUTROPHIL APOPTOSIS ON ENDOTHELIAI CELLS

口腔长期炎症机制：中性粒细胞凋亡对内皮细胞的影响

• 批准号: 12671962
• 负责人: IWASE Masayasu
• 金额: $ 2.24万
• 依托单位: SHOWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671962/
• 关键词: APOPTOSIS; FAS; NEUTROPHILS; ENDOTHELIAL CELLS; ADOHESIVEMOLECULE; CYTOKINE; CHEMOKINE; NSAID

1) Endothelial cells, HUVECs, constitutively expressed Fas antigen on cell surface.2) HUVECs were induced apoptosis by the addition of anti-Fas, CH-11.3) When HUVECs and neutrophils were co cultured in the presence of anti-Fas, they lost adhesive function each other.4) When neutrophils were treated with anti-Fas, the expressions of CD11b and CD18 decreased in its cell surface. Therefore, adhesive dysfunction was dependent on adhesive molecule on neutrophils.5) Because chemokine and inflammatory cytokine reduced Fas-mediated apoptosis on HUVECs and neutrophils, these agents maintained neutrophil function in the acute inflammatory tissues.6) In contrast, NSAIDs enhanced Fas-mediated apoptosis on HUVECs and neutrophils, then this event was new a strategy for anti-infiammatory function of NSAIDs.

1)抗Fas抗体、CH-11可诱导HUVECs凋亡;抗Fas抗体处理后，HUVECs与中性粒细胞失去粘附功能;抗Fas抗体处理后，中性粒细胞表面CD 11b、CD 18表达下降。5）由于趋化因子和炎性细胞因子减少Fas介导的HUVECs和中性粒细胞凋亡，这些药物维持了急性炎症组织中中性粒细胞的功能。6）相反，NSAID促进Fas介导的HUVECs和中性粒细胞凋亡，这一事件是NSAID抗炎作用的新策略。

项目成果

Toshiaki Takemi: "Lymphonode metastasis in squamous cell carcinoma of the oral cavity"Oral Oncology. 4B. 209-211 (2001)

Toshiaki Takemi：“口腔鳞状细胞癌的淋巴结转移”口腔肿瘤学。

Masayasu Iwase: "Hypercalcemia in patients with oral squamous cell carcinoma"Clinical Oral Investigation. 5・2. 194-198 (2002)

Masayasu Iwase：“口腔鳞状细胞癌患者的高钙血症”临床口腔研究 194-198（2002）。

Hitoshi Watanabe, et al: "Role of imterleukin-8 secreted from human oral squamous cell carcinoma cell lines."Oral Oncology. (in press).

Hitoshi Watanabe 等人：“人口腔鳞状细胞癌细胞系分泌的 imterleukin-8 的作用。”口腔肿瘤学。

岩瀬正泰: "口腔扁平上皮癌細胞のFas誘導アポトーシスに対する抗癌剤の効果"日本頭頸部腫瘍学会誌. 27・4. 239-243 (2001)

Masayasu Iwase：“抗癌药物对Fas诱导的口腔鳞状细胞癌细胞凋亡的影响”日本头颈肿瘤学会杂志27・4（2001）。

Masayasu Iwase: "Hypercalcemic complication in patients with oral squamous cell carcinoma"International Journal of Oral and Maxillofacial Surgery. (in press).

Masayasu Iwase：“口腔鳞状细胞癌患者的高钙血症并发症”国际口腔颌面外科杂志。