Measurement of plasma sphingosine 1-phosphate

血浆 1-磷酸鞘氨醇的测定

• 批准号: 08672635
• 负责人: YATOMI Yutaka
• 金额: $ 1.41万
• 依托单位: Yamanashi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672635/
• 关键词: sphingosine 1-phosphate; sphingosine; plasma; platelet; スフィンゴシン-1ーリン酸

Although sphingosine 1-phosphate (Sph-1-P) is reportedly involved in diverse cellular processes and the physiological roles of this bioactive sphingolipid have been strongly suggested, few studies have revealed the presence of Sph-1-P in human samples, including body fluids and cells, under physiological conditions. In this study, we identified Sph-1-P as a normal constituent of human plasma and serum. The Sph-1-P levels in plasma and serum were 191 (]SY.+-。[) 79 and 484 (]SY.+-。[) 82 pmol/ml (mean (]SY.+-。[) SD,n=8), respectively. Furthermore, when Sph-1-P was measured in paired plasma and serum samples obtained from 6 healthy adults, the serum Sph-1-P/plasma Sph-1-P ratio was found to be 2.65 (]SY.+-。[) 1.26 (mean (]SY.+-。[) SD). It is most likely that the source of discharged Sph-1-P during blood clotting is platelets, because platelets abundantly store Sph-1-P compared with other blood cells, and release part of their stored Sph-1-P extracellularly upon stimulation. We also studied Sph-1-P-related metabolism in plasma. [<@D13@>D1H] Sph was stable and not metabolized at all in plasma, but was rapidly incorporated into platelets and metabolized mainly to Sph-1-P in platelet-rich plasma. [<@D13@>D1H] Sph was found to be unchanged in plasma, revealing that plasma does not contain the enzymes needed for Sph-1-P degradation. In summary platelets can convert Sph into Sph-1-P,and are storage sites for the latter in the blood. In view of the diverse biological effects of Sph-1-P,the release of Sph-1-P from activated platelets may be involved in a variety of physiological and pathophysiological processes, including thrombosis, hemostasis, atherosclerosis and wound healing.

尽管据报道 1-磷酸鞘氨醇 (Sph-1-P) 参与多种细胞过程，并且这种生物活性鞘脂的生理作用已被强烈暗示，但很少有研究揭示生理条件下人体样本（包括体液和细胞）中存在 Sph-1-P。在这项研究中，我们确定 Sph-1-P 是人血浆和血清的正常成分。血浆和血清中的Sph-1-P水平分别为191 (]SY.+-。[) 79和484 (]SY.+-。[) 82 pmol/ml (平均值(]SY.+-。[) SD,n=8)。此外，当对 6 名健康成年人的配对血浆和血清样本中的 Sph-1-P 进行测量时，发现血清 Sph-1-P/血浆 Sph-1-P 比率为 2.65 (]SY.+-。[) 1.26（平均值 (]SY.+-。[) SD）。血液凝固过程中排出的 Sph-1-P 的来源很可能是血小板，因为与其他血细胞相比，血小板储存了大量的 Sph-1-P，并在受到刺激时将部分储存的 Sph-1-P 释放到细胞外。我们还研究了血浆中 Sph-1-P 相关的代谢。 [<@D13@>D1H] Sph 很稳定，在血浆中根本不代谢，但很快融入血小板，并在富含血小板的血浆中主要代谢为 Sph-1-P。 [<@D13@>D1H] Sph 在血浆中未发生变化，表明血浆中不含有 Sph-1-P 降解所需的酶。综上所述，血小板可以将Sph转化为Sph-1-P，并且是后者在血液中的储存场所。鉴于Sph-1-P的多种生物学作用,活化血小板释放Sph-1-P可能参与多种生理和病理生理过程,包括血栓形成、止血、动脉粥样硬化和伤口愈合等。

项目成果

Yatomi, Y., Yamamura, Y., Ruan, F., Kume, S., Ozaki, Y., and Igarashi, Y.: "N,N-Dimethylsphingosine 1-phosphate activates human platelets." FEBSLett.417. 341-344 (1997)

Yatomi, Y.、Yamamura, Y.、Ruan, F.、Kume, S.、Ozaki, Y. 和 Igarashi, Y.：“N,N-二甲基鞘氨醇 1-磷酸可激活人血小板。”

Yutaka Yatomi: "Sphingosine 1-phosphate,a bioactive sphimgolipid abundantly stored in platelets,is a normal constituent of human plasma and serum" Journal of Biochemistry.

Yutaka Yatomi：“1-磷酸鞘氨醇是一种大量储存在血小板中的生物活性鞘脂，是人体血浆和血清的正常成分”《生物化学杂志》。

Yatomi Y: "Sphingosine 1-phosphate,a bioactive sphingolipid abundently stored in platelets,is a normal constituent of human plasma and serum" Journal of Biochemistry. 121. 969-973 (1997)

Yatomi Y：“1-磷酸鞘氨醇是一种大量储存在血小板中的生物活性鞘脂，是人体血浆和血清的正常成分”《生物化学杂志》。

Yatomi Y: "Sphingosine 1-phosphate,a,bioactive sphingolipid abundantly stored in platelets,is a normal constituent of human plasma and serum" Journal of Biochemistry. 121. 969-973 (1997)

Yatomi Y：“1-磷酸鞘氨醇，一种生物活性鞘脂，大量储存在血小板中，是人体血浆和血清的正常成分”《生物化学杂志》。

Yatomi Y: "N,N-Dimethylsphingosine 1-phosphate activates human platelets" FEBS Letters. 417. 341-344 (1997)

Yatomi Y：“N,N-二甲基鞘氨醇 1-磷酸激活人类血小板”FEBS 快报。